Browsing by Author "Oyabambi, A.O"

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    Adenopus breviflorus restores glucose-6-phosphate dehydrogenase suppression caused by clozapine in female Wistar rats
    (Pharma Research Library, India., 2020) Oyabambi, A.O; Okesina, A.A; Saliu, B.S; Salawudeen, S.A; Areola, D.E
    Oxidative damage is an important feature of cardiometabolic syndrome. Clozapine (CLO) is a typical antipsychotic medication that alters protein and lipid metabolism with consequent oxidative stress. The G6PD-dependent antioxidant barrier is an important cellular defense against oxidative damage. Nevertheless, Adenopus Breviflorus (AEAB) has been shown to contain beneficial antioxidant phytochemicals and inhibits mitochondrial lipid peroxidation. This study therefore aimed at testing AEAB fruit aqueous extract antioxidant property in CLO-induced redox imbalance. Twenty male Wistar rats weighing between 135-140 g were used for the experiment. They were grouped into Control (CTR; Standard feed and Water), Clozapine only (CLO; 25mg/kg of Clozapine), Extract only (AEAB; 200mg/kg of AEAB) and Clozapine +Extract (CLO+AEAB; 25 mg/kg of Clozapine for first twenty-eight days and AEAB for the last fourteen days), where n=5. All administrations were done orally, and the experiment lasted for forty-two days after a week of acclimatization. The animals were euthanized with 0.2ml of ketamine hydrochloride, blood samples were collected measured for plasma G6PDH, Catalase, Sodium Dismutase, NADPH, GPX, and GSSG using standard spectrophotometric method. The results showed that CLO reduced plasma G6PD, NADPH, GPx, and cardiac NADPH. There was a slight reduction in cardiac G6PD, SOD, GSSG, and GPx with CLO treatment. However, AEAB reversed the alterations caused by CLO treatment in the heart and plasma of animals.AEAB elicits considerable antioxidant activity by limiting the oxidant effects of CLO in the plasma, heart, and plausibly other tissues. AEAB may be considered for therapy in the treatment of oxidative stress-related diseases.
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    Adverse effect of graded Ciprofloxacin oral intake in male Sprague-Dawley rats.
    (African Association of Physiological Sciences, 2020) Igbayilola, Y.D; Saka, W.A; Aina, O.S; Mofolorunso, A.M; Oyabambi, A.O; Morakinyo, A.O
    : Ciprofloxacin (CF) is a very popular antibiotics used for therapeutic prospects. This study was investigated to determine the effect of graded CF oral intake on lipid profile, hepato-reno, antioxidant and cardiovascular functions in Sprague-Dawley rats. Methods: Male Sprague-Dawley rats (120-150g) were grouped into 4 equal groups. Group A; control, received 1 ml of normal saline/100g bw, group B, C and D received CF orally at 5, 10 and 20mg/kg respectively. After 7days daily treatments, blood sample was collected via retro-orbital puncture to obtain serum sample for the determination of cholesterol (CHOL), triglyceride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL). Urea, creatinine, aspartate amino transferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) were investigated. Superoxide dismutase (SOD), reduced glutathione (GSH), catalase (CAT), malonaldehyde (MDA), blood pressure (BP) and heart rate (HR) were measured via femoral cannulation under anesthesia using urethane and 1% α-chloralose. Results: There was a significant decrease (p<0.05) in CHOL in group C while TG, LDL, urea significantly reduced in groups B and C and significantly increased in group D (p<0.05). Creatinine, ALT and ALP upregulated in group D (p<0.05) while AST downregulated in groups C and D. GSH and CAT downregulated in groups B, C and D while SOD reduced significantly (p<0.05) in groups B and D. MDA downregulated in groups B and C and upregulated (p<0.05) in group D (p<0.05). BP and HR significantly increased in groups B, C and D compared with control (P<0.05). Conclusion: This study showed that oral CF intake at 5mg/kg and 10mg/kg had no adverse effect on lipid profile, hepato-reno and antioxidant functions. However, CF at 20mg/kg may predispose to dyslipidaemia, hepato-reno dysfunction and increased oxidative stress. CF at 20mg/kg may exert a deleterious effect cardiovascular function.
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    Anacardium occidentale and Psidium guajava effectively reduced ethanol- induced gastric ulcer in male Wistar rats.
    (College of Health Sciences, 2016) Oyabambi, A.O; Oyeniyi, P.O; Ojulari, L.S; Oladoye, O.D
    Ulcer is one of the world‟s major gastrointestinal disorders affecting a good percentage of the populace in industrialised and developing countries. Plant extracts are some of the most attractive sources of new drugs and have shown promising results for the treatment of gastric ulcer as they possess a variety of natural therapeutic products. This study aimed to look at the effect of combining Anacardium Occidentale and Psidium Guajava on the management of gastric ulcer. The extracts of the leaves of Anacardium occidentale and Psidium guajava were tested in ethanol-induced ulcer models in rats. Oral administration of these plant extracts and reference drug was done for two weeks for 30 male Wistar rats divided into five (5) groups. The treatment with the combination of the extract stock solutions at a dose of 150mg/kg each exhibited a significant (p < 0.05) decrease in gastric volume, 2.1x10-3 mEq/L and ulceration, 2.0±0.26 than the individual extract groups when compared to control 4.0x10-3 mEq/L and 5.2±0.60 respectively while cimetidine at the dose tested had the highest percentage reduction. The efficacy of the extracts presented here confirms that the combination of Anacardium occidentale and Psidium guajava possess better synergistic ulcer healing and gastro protective effects than the individual components. This affirms their efficacy in the management of ulcers
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    Assessment of Emergency Preparedness and Response Among Police Officers and Men in Ilorin, Nigeria
    (Library and Publication Committee, University of Ilorin, 2018) Oladoye, O.D; Uthman, M.M.B; Oladiji, F; Ameen, H.A; Oyabambi, A.O
    The Police force is crucial in the local and national level emergency response through the 21st century and beyond. Emergency preparedness will be viewed as a protective process which encompasses all measures taken before a disastrous event aimed at minimizing the loss of life, disruption of critical services and damage to infrastructure and the environment, enabling governments, communities and individuals to respond rapidly and efficiently to disasters. The study assessed the knowledge, preparedness and response of police personnel towards emergency in Ilorin. A descriptive cross-sectional study of 400 policemen and officers was carried out, utilizing a semi-structured questionnaire in selected police formations. Descriptive and inferential statistics was carried out using the Statistical Package for Social Science (SPSS) version 20 with level of significance set at p<0.05. It revealed a higher proportion: 221 (55.2%) of the respondents with average knowledge of emergencies, good level of emergency preparedness: 207 (51.8%) and average level of response to emergencies: 334 (83.5%). The study also showed that worn-out equipment, inadequate facilities and insufficient relevant training were the major challenges that the police force face in emergency preparedness and response activities in Ilorin. The need to constantly train policemen in view of the dynamic and evolving nature of emergencies in Nigeria is therefore advocated. http//www.unilorin.edu.ng/centrepoint Volume 24, No.1, pages 85-100
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    Awareness and perception about pentavalent vaccine among women of reproductive age group in north central Nigeria
    (College of Health Sciences, 2017) Oyabambi, A.O; Bolarinwa, O.A; Adebayo, O; Emerenini, F.C
    Introduction of pentavalent vaccine in the year 2012 to Nigerian childhood vaccination programme was adjudged as a major advancement in the field of childhood vaccination. However, efforts made to achieve universal coverage was quite challenging with pockets of outbreak of vaccine-preventable diseases in the country. This was attributed to low awareness, perception and low uptake of vaccination. Therefore, the level of awareness and perception of pentavalent vaccines and its determinants among women of reproductive age group in Ilorin West LGA of Kwara state was studied using a semi-structured interviewer administered questionnaire in January 2013. A community-based descriptive cross-sectional study using multistage sampling technique in Ilorin West local government area of Kwara State was carried out. The Fischer’s formula was used to determine the minimum sample size of 400 while the results were analyzed with EPI INFO version 6 software package. Age, marital status, educational level and occupation are significantly associated with individual awareness and perception on pentavalent vaccine, p< 0.05. A total of 77% of the subjects were aware of the introduction of the vaccines into the National Programme on Immunization while about 92.8% perceived that the vaccine was beneficial. The level of awareness and perception of pentavalent vaccine was high among the age group of 20-39. It was also higher among those with higher level of education, among civil servants and students. The major source of their awareness was health workers. There is therefore a need for improvement of awareness and perception among all categories of women, especially those in the child bearing age, and health workers involved in immunization activities in order to improve this vaccination uptake
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    Biochemical and antioxidant effects of Talinum triangulare (Water Leaf) in female Sprague -Daley rats.
    (The Society of Basic and Applied Physiology, INDIA, 2017) Igbayilola, Y.D; Morakinyo, A.O; Ewetayo, A.W; Oyabambi, A.O
    Therapeutic properties of medicinal plants are very useful in the treatment and healing various diseases and the advantaged of these plants is been 100% natural with little or no side effects. The present study seeks to investigate the biochemical and antioxidant effects of aqueous leaf extract of Talinum triangulare (water leaf) in female Sprague -Dawley rats. Twenty Four (24) female Sprague -Dawley rats weighing 150-180gram were grouped into 4 of 6 rats each. Group A: control; administered (10ml/kg body weight) of normal saline. The other groups were administered the aqueous leaf extract of Talinum triangulare thus: group B - 50mg/kg, group C - 100mg/kg, and group D – 150mg/kg. After fourteen (14) days, blood samples were collected for lipid and antioxidant analysis. The results revealed significant increase in AST (aspartate amino transferase) at 150ml/kg, when compared with the group A S(P>0.05). The ALT (alanine amino transferase) was significantly decreased (P>0.05) at 100ml/kg when compared with group A and group B with significant elevation at 150ml/kg. The ALP (alkaline phosphatase) was significantly increased in all the tested doses (P>.0.05). Results from the lipid profile analysis showed significant decrease in all the doses tested (P>0.05) for cholesterol (CHOL), triglyceride (TG) and high density lipoprotein (HDL) levels except for CHOL and HDL which showed significant increase (P>0.05) at 150ml/kg. The low density lipoprotein (LDL), protein, and albumin levels showed significant increase in the tested doses expect, protein and albumin which was significantly decreased (P>0.05) at 100ml/kg and 150ml/kg. Also the creatinine level was significantly decreased in all the doses tested (P>0.05). The antioxidant assay revealed significant increase in glutathione, GSH, superoxide dismutase, SOD and Catalase, CAT (P>.05) at all doses, expect SOD which was significantly decreased at 150mg/kg. The malondialdehyde, MDA was significantly decreased (P>0.05) in all the tested doses. Our findings showed that aqueous leaf extract of Talinum triangulare administration at 50mg/kg, 100mg/kg and 150mg/kg have no adverse effect on renal functions. However, at 150ml/kg the aqueous leaf extract of Talinum triangulare gave rise to increase in liver enzymes' activity, increased low density lipoprotein as well as oxidative radical productions. Finally excessive intake should be avoided since it is dose dependent because of its ability to increase risk of obesity, cardiovascular disorder and liver damage.
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    Cornsilk methanolic extract improves oxidative stress and inflammatory responses in rats excision wound model
    (University of Peradeniya, Sri Lanka., 2021) Oyabambi, A.O; Nafiu, A.B; Okesina, A; Babatunde, S.S; Dominic, E.K; Oreoluwa, D.O
    Anti-inflammatory and antioxidant activities of corn silk (Stigma maydis of Zea mays L.) extract were investigated in excision wound model. Twenty male Wistar rats (130-150g) were grouped into four (n=5/group): Control rats received 0.5 mL distilled water, the experimental groups received distilled water (0.5 mL), aqueous corn-silk extract (ACSE; 500 mg/kg) and methanolic corn-silk extract (MCSE; 500 mg/kg), twice/day orally, three weeks before and three weeks after they had 1.5 × 1.0 cm excision wound. Rats were sacrificed and blood was collected. Serum was separated while wound tissues were removed, homogenised and supernatant was stored. Antioxidant enzymes, malondialdehyde, hydroxyproline, cyclooxygenase-2 and NO were measured in wound tissues colorimetrically, while serum cytokines were measured using enzyme linked immunosorbent assay. MCSE significantly (p˂0.05) increased glutathione peroxidase, superoxide dismutase, catalase, hydroxyproline and total protein content but decreased significantly (p˂0.05) malondialdehyde, cyclooxygenase-2, IL-1β, TNF-α, and IFN-γ when compared with untreated wound. ACSE increased significantly (p<0.05) glutathione peroxidase and superoxide dismutase but decreased significantly (p<0.05) malondialdehyde and cyclooxygenase-2. However, it produced no significant effect on catalase, IL-1β, TNF-α, and IFN-γ when compared with untreated wound. MCSE attenuated oxidative and inflammatory responses in excision wound rat model
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    Effects of albendazole administration on the testicular histology of adult Wistar rats
    (Society of Experimental and Clinical Anatomist of Nigeria (JECA), 2013) Omotoso, G.O; Alabi, A.S; Oyabambi, A.O
    The testicular cytoarchitectural effects of albendazole administration were studied in adult Wistar rats. Materials and Methods: The animals were grouped into three groups, with six (6) rats per group. While animals in the control group A were given water, the treatment groups B and C received 15 mg/kg and 30 mg/kg respectively of albendazole orally for three consecutive days. The animals were sacrificed by cervical dislocation about 24 hours after drug administration, and the testes removed, fixed in 10% formal saline and processed for histological consideration, using Haematoxylin and Eosin stains.Results: Varying degrees of histological changes were noticed in the seminiferous tubules, which were marked in the group that received the higher dose of the drug. Conclusion: These structural distortions and degenerations of testicular tissues that occurred after albendazole administration are dose-dependent, and could affect reproductive functions in males
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    Perception of cheek dimples amongst the Yorubas in Nigeria
    (College of Health Sciences, 2014-07) OMOTOSO, G.0; Ojulari, L.S; Oyabambi, A.O
    The attitude of people toward cheek dimples differs based on gender, culture an geographical location. This survey was done to determine the attitude of people of the same cultural background towards the presence of cheek dimples. The respondents, which were randomly selected, were Yoruba speaking indigenes of southwest Nigeria. They were served with structured questionnaires by hand. Out of 500 questionnaires, 411 were returned correctly filled, representing 82.2% response rate. The age range of respondent was between 16 and 35 with majority (90.3%) between ages 16 and 25. Only 108 out 411 (26.3%) had cheek dimples, out of which 63 (58.3%) were females and 45 (41.7%) were males, with most dimples occurring between ages 16 and 20 (53.7%). Most of the respondents like cheek dimples, and many that did not have cheek dimples especially females, signified their interest in having dimples. Willingness to have cheek dimples were due to some importance attached to dimples such as its aesthetic value, admiration and the fact that those who have cheek dimples appear younger than their age.
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    Processing method influences the effect of cassava (Manihot esculenta) consumption on blood lipid profile in rats.
    (International Journal of the Nigerian Society, 2014) Oyabambi, A.O; Ibitoye, C.M; Biliaminu, S.A; Okesina, K.B; Nyamngee, A
    Cassava is consumed in many tropical countries and is fast becoming the major raw material of many diets, especially in the bakery, brewery and pharmaceutical industries. This study was aimed at determining the effects of various formulations of Cassava feeds on blood lipid profile in rats. Forty adult Wistar rats weighing between 120 and 150 g were divided into 4 groups of 10 rats each. The rats were acclimatized for one week; fed with different formulations of Cassava feeds ad libitum, after which the rats were allotted into groups. Group 1 was fed on Garri; group 2 on Cassava flour; group 3 on Tapioca, and the control group (group 4) on normal chow. After 4 weeks, each animal was anaesthetized by exposure to chloroform vapour and blood samples collected by cardiac puncture. Group 1 diet (Garri) significantly increased HDL-cholesterol (HDL-C) concentration and significantly decreased LDL-cholesterol (LDL-C) concentration (p<0.05) compared with the control group. In Group 2 (Cassava flour), there was significant increase in the concentration of HDL-C (p<0.05). Group 3 (Tapioca) had a significant increase in TC, TG, LDL-C concentrations (p<0.05), but an insignificant increase in HDL-C concentration (p<0.05) when compared with the control. In conclusion, our findings suggest that Garri and Cassava flour but not Tapioca enhanced HDL-C in the blood, with Garri proving to be the better of the two.
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    Role of high-density lipoprotein in the ameliorative activity of Bryophyllum pinnatum in male Wistar rats fed a high fat diet.
    (Faculty of Basic Medical Sciences, Lagos State, University College of Medicine, Lagos, Nigeria, 2020) Oyabambi, A.O; Saliu, S.B; Erdoo, A; Akinsanya, Z.O
    Atherosclerosis, a condition connected with fat-rich diet is a leading cause of death worldwide. It has been strongly associated with dysmetabolic, dyslipidemic and inflammatory conditions with sparingly effective available therapeutic measures. High-density lipoprotein (HDL-C), the agreeably beneficial chylomicron, has been linked to improved glycemic control, a finding being growingly investigated. Bryophyllumpinnatum (BP) aqueous leaf extract or its steroidal derivative is well known for antidiabetic and/or anti-inflammatory potential. This study therefore investigated the effect of BPon circulating lipids, inflammatory response markers, and glycemic control in male Wistar rats fed a high-fat diet. Twenty-four male Wistar rats (120-130 g) were randomly selected into four groups (n=6) and fed with rat chow, high-fat diet (HFD), HFD  +  BP (250 mg/kg), and rat chow + BP (250 mg/kg)for six weeks after two weeks of acclimatization. High-fat-fed rats showed reduced HDL-C, increased fasting glycemia, plasma triglycerides (TG), low-density-lipoprotein cholesterol (LDL-C), C-reactive protein (CRP), and uric acid (UA). The total cholesterol was not altered in HFD group. The BP leaf aqueous extract however augmented HDL-C with accompanying improved fasting glycemia and attenuated plasma TG, TC, LDL-C, CRP, and UAin high fat fed rats. The extract was demonstrated to confer protection against lethal accumulation of atherogenic lipids and cause the augmentation of HDL-C with a plausible resultant improvement of glycemic control and amelioration of inflammatory response in high-fat diet-fed animals.