Adverse effect of graded Ciprofloxacin oral intake in male Sprague-Dawley rats.
No Thumbnail Available
Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
African Association of Physiological Sciences
Abstract
: Ciprofloxacin (CF) is a very popular antibiotics used for therapeutic prospects.
This study was investigated to determine the effect of graded CF oral intake on lipid profile,
hepato-reno, antioxidant and cardiovascular functions in Sprague-Dawley rats. Methods: Male
Sprague-Dawley rats (120-150g) were grouped into 4 equal groups. Group A; control, received
1 ml of normal saline/100g bw, group B, C and D received CF orally at 5, 10 and 20mg/kg
respectively. After 7days daily treatments, blood sample was collected via retro-orbital puncture
to obtain serum sample for the determination of cholesterol (CHOL), triglyceride (TG), high
density lipoprotein (HDL) and low density lipoprotein (LDL). Urea, creatinine, aspartate amino
transferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) were
investigated. Superoxide dismutase (SOD), reduced glutathione (GSH), catalase (CAT),
malonaldehyde (MDA), blood pressure (BP) and heart rate (HR) were measured via femoral
cannulation under anesthesia using urethane and 1% α-chloralose. Results: There was a
significant decrease (p<0.05) in CHOL in group C while TG, LDL, urea significantly reduced
in groups B and C and significantly increased in group D (p<0.05). Creatinine, ALT and ALP
upregulated in group D (p<0.05) while AST downregulated in groups C and D. GSH and CAT
downregulated in groups B, C and D while SOD reduced significantly (p<0.05) in groups B and
D. MDA downregulated in groups B and C and upregulated (p<0.05) in group D (p<0.05). BP
and HR significantly increased in groups B, C and D compared with control (P<0.05).
Conclusion: This study showed that oral CF intake at 5mg/kg and 10mg/kg had no adverse
effect on lipid profile, hepato-reno and antioxidant functions. However, CF at 20mg/kg may
predispose to dyslipidaemia, hepato-reno dysfunction and increased oxidative stress. CF at
20mg/kg may exert a deleterious effect cardiovascular function.
Description
Keywords
Ciprofloxacin, lipid profile, oxidative stress, liver, renal function, cardiovascular system
Citation
A.O. Oyabambi et. al