Cornsilk methanolic extract improves oxidative stress and inflammatory responses in rats excision wound model
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
University of Peradeniya, Sri Lanka.
Abstract
Anti-inflammatory and antioxidant activities of corn
silk (Stigma maydis of Zea mays L.) extract were investigated
in excision wound model. Twenty male Wistar rats (130-150g)
were grouped into four (n=5/group): Control rats received 0.5
mL distilled water, the experimental groups received distilled
water (0.5 mL), aqueous corn-silk extract (ACSE; 500 mg/kg)
and methanolic corn-silk extract (MCSE; 500 mg/kg), twice/day
orally, three weeks before and three weeks after they had 1.5 × 1.0
cm excision wound. Rats were sacrificed and blood was collected.
Serum was separated while wound tissues were removed,
homogenised and supernatant was stored. Antioxidant enzymes,
malondialdehyde, hydroxyproline, cyclooxygenase-2 and NO
were measured in wound tissues colorimetrically, while serum
cytokines were measured using enzyme linked immunosorbent
assay. MCSE significantly (p˂0.05) increased glutathione
peroxidase, superoxide dismutase, catalase, hydroxyproline
and total protein content but decreased significantly (p˂0.05)
malondialdehyde, cyclooxygenase-2, IL-1β, TNF-α, and
IFN-γ when compared with untreated wound. ACSE increased
significantly (p<0.05) glutathione peroxidase and superoxide
dismutase but decreased significantly (p<0.05) malondialdehyde
and cyclooxygenase-2. However, it produced no significant
effect on catalase, IL-1β, TNF-α, and IFN-γ when compared with
untreated wound. MCSE attenuated oxidative and inflammatory
responses in excision wound rat model
Description
Keywords
Corn silk extract, antioxidant enzymes, antiinflammation, cytokines, wound healing
Citation
A.O. Oyabambi et. al