Browsing by Author "Ojulari, L.S.,"
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Item ABO/rhesus blood group systems are not clinical indicators of male baldness.(College of Medicine, Ambrose Alli University, 2017) Ayinde, T.O.,; Sanni, M.A.,; Afodun, A.M.,; Ojulari, L.S.,; Jimoh-Abdulghaffaar, H.O.,; Ayinla M.T.,; Abdulazeez, F.I.,; Abdulkareem, S., .; Abdulrahim, H.A.; Samotu, KBackground: Several disease entities have been linked to the ABO/Rh blood group systems.Baldness or alopecia is the partial or complete lack of hair on the head and/or body. Major advances have been achieved in understanding principal elements of the androgen metabolism involved in the pathogenesis of alopecia, but not much preliminary work has been done in its relationship to blood types. Aim: This study is aimed to determine if there is any association between blood types and male baldness. Methods: 400 male subjects (25-60 years)at Sobi Specialist Hospital Alagbado, Ilorin, kwara State, Nigeria were recruited into the study(200for control and 200for baldness).Blood sample was collectedfrom each subjectfor blood grouping estimation, following the completion of a questionnaire containing information about baldness and haematological profile. Result: The distribution ofphenotypic frequencies of ABO group in the control samples were 26.00 28.00 4.000 and 42.0% for groups A, B, AB and O, respectively, while 92.0 00 of the subjects were Rh (D) positive and 8.00% Rh(d) negative. And for the baldness, they were 26.000, 26.00 4.000 and 44% for A, B, AB, and O respectively; while Rh (D) positive were 94.000 and Rh (d) negative were 6.0%. The overall result is statistically insignificant (P>O.OS) using Pearson Chi-square. Conclusion: The result reflects an absolute parallel relationship between baldness and ABO/Rhesus blood group systems. Thus, ordering for blood group assessment during routine hair clinic as part of ancillary investigation should be discouraged, except if other interests arise. Keywords: ABO, rhesus, blood group, baldnessItem Androgenic effects of aqueous extract of moringa oleifera in male Wistar rats.(School of Medicine and Health Sciences, University of Papua, New Guinea., 2022) Ayinla, M.T.,; Sikirulai, M.A.,; Ayinde, T.O., .; Ojulari, L.S.,; Owoyele, V.B.,; Asuku, A.O.,; Adebisi, R.O.,; Badmus, O.A.,; Murthy, R.K.Androgenic effects of Aqueous Seed-extract of Moringa oleifera (ASMO) in male Wistar rats were investigated. Eighteen (18) male Wistar rats weighing 200-240g were used for this study. The rats were divided into three (3) groups: Control (Group 1) that received 10 ml/kg of normal saline, Group 2: received low dose of ASMO (200mg/kg), and Group 3: received high dose of ASMO (500mg/kg). The animals were treated for twenty-eight days. On the 29th day, the rats were sacrificed and the testes were carefully removed for semen and biochemical analysis. Body weight, reproductive and vital organ weights were determined. Sperm parameters (motility, morphology, count and viability), tissue testosterone, luteinizing hormone (LH), Malondialdehyde (MDA) and Catalase were also determined using standard methods. Data were analysed using one-way ANOVA followed by Duncan new multiple range post hoc test. The result showed ASMO significantly increased (p<0.05) the final body weight, weight of reproductive and vital organs. Moreover, 200mg/kg body weight dose of ASMO significantly increased (p<0.05) the sperm parameters but 500mg/kg body weight dose significantly decreased it. ASMO also caused a significant dose dependent increase (p<0.05) in testosterone and catalase level but a significant decrease (p<0.05) in MDA and LH level compared with the control. In conclusion, this study revealed that ASMO has androgenic effects in male rats and thus provides a basis for the traditional use of Moringa oleifera in the management of male sexual disordersItem Correlation between testosterone and prostate specific antigen in male baldness in (Government Specialist Hospital, Sobi) Ilorin West, Local Government Area, Kwara State, Nigeria(Faculty of Basic Medical Sciences, Lagos State University College of Medicine, 2017) Ojulari, L.S.,; Ayinde, T.O.,; Sanni, M.,; Olayaki, L.A.,; Quadri, K.,; Egbeche, E.; Dangana, E.O.Background: Male pattern hair loss (MPHL) is influenced by increased androgenic activities in the body, prostatic diseases has been linked with high androgenic activities, especially Dihydrotestosterone (DHT) that influences the growth of hair on the body and prostatic cells. This study aimed to check the relationship between androgenic activity with respect to prostatic specific antigen (PSA) expression in bald and non-bald men. Methods: Seventy (70) subjects participated in this study at the Kwara State Specialist Hospital, Ilorin. These subjects were divided into two equal groups of 35 subjects each. Group A- served as control subject; men with no apparent baldness while group B- comprised of men with apparent baldness. A questionnaire was given to each subject to ensure participation was voluntary. Blood sample was collected from each subject to be used for hormonal assay to check for PSAand TTconcentrations using appropriate kits. Results: Plasma testosterone significantly increased in men with apparent baldness (P≤0.001) compared to control subjects. PSA also increased insignificantly in men with apparent baldness which might be indicative of probable occurrence of prostate carcinoma or hyperplasia in men with apparent baldness. Conclusion: This study revealed that prostate carcinoma might occur in male with apparent baldness due to progressive increase in PSA along with increased androgenic activityItem Effect of Chronic Administration of Caffeine on Renal Function in Male Wistar Rats(Society for the Conservation of Phytofuels and Sciences., 2022) Ayinde, T.O.,; Ojulari, L.S.,; Afodun, A.M.,; Balogun, M.E.,; Faramade, A.A.,; Raji, A.O.,; Liadi, M.T.,; Belewu, M.A.Caffeine is one of the most widely consumed of all beverages in the World that is capable of stimulating the nervous system, producing alertness of short duration without much regards to organ affectations. Twenty-one (21) male Wistar rats were randomly divided into three (3) groups of 7 rats each as follows: Group I was the control and was given 0.5 ml of NaCl, Groups II and III were administered 0.38 mg/kg and 1.14 mg/kg of caffeine respectively for 14 days. Assessment of renal function was evaluated by collection of urine and blood samples for urinalysis and electrolytes, urea, and creatinine analysis respectively. Urinalysis result precisely showed that protein was positive (+) with a pH of 5.5 in the control group. The LD animals revealed bilirubin (2+), protein (+) with a pH of 5.0. The HD group showed bilirubin (2+), protein (3+) with a pH of 3.5. There was significant (p<0.05) increase in serum urea and creatinine of LD and HD. The serum sodium level was significantly (p<0.05) reduced in LD, while serum potassium level was significantly (p<0.05) increased in the HD group. Chronic consumption of caffeine should be with moderation and at a very lower dose to prevent gradual untoward effects on functions of the kidneys, especially in an already compromised renal status.Item Effect of Chronic Administration of Caffeine on Renal Function in Male Wistar Rats.(Society for the Conservation of Phytofuels and Sciences., 2022) Ayinde, T.O.,; Ojulari, L.S.,; Afodun, A.M.,; Balogun, M.E.,; Faramade, A.A.,; Raji, A.O.,; Liadi, M.T.,; Belewu, M.A.Caffeine is one of the most widely consumed of all beverages in the World that is capable of stimulating the nervous system, producing alertness of short duration without much regards to organ affectations. Twenty-one (21) male Wistar rats were randomly divided into three (3) groups of 7 rats each as follows: Group I was the control and was given 0.5 ml of NaCl, Groups II and III were administered 0.38 mg/kg and 1.14 mg/kg of caffeine respectively for 14 days. Assessment of renal function was evaluated by collection of urine and blood samples for urinalysis and electrolytes, urea, and creatinine analysis respectively. Urinalysis result precisely showed that protein was positive (+) with a pH of 5.5 in the control group. The LD animals revealed bilirubin (2+), protein (+) with a pH of 5.0. The HD group showed bilirubin (2+), protein (3+) with a pH of 3.5. There was significant (p<0.05) increase in serum urea and creatinine of LD and HD. The serum sodium level was significantly (p<0.05) reduced in LD, while serum potassium level was significantly (p<0.05) increased in the HD group. Chronic consumption of caffeine should be with moderation and at a very lower dose to prevent gradual untoward effects on functions of the kidneys, especially in an already compromised renal status.Item Effect of gliclazide on uric acid and c-reactive protein in alloxan-induced diabetic rats.(University of Ilorin, 2012) Ojulari, L.S.,; Biliaminu, S.A.,; Dangana, E.O.,; Abdulazeez, F.I.,; Ayinde, T.O. .; Adegoke, O.ADiabetes mellitus represent one of the greatest threats to modern global health.Item Effect of jatropha curcas seed aqueous extract on quality of semen and reproductive hormones in male Wistar rats(Society of Basic and Applied Physiology, 2022) Ayinde, T.O.,; Abubakar, H.O.,; Ojulari, L.S.,; Balogun, M.E.,; Liadi, M.T.,; Olayaki, L.A.,; Belewu, M.A.,; Afodun, A.MBackground: Jatropha curcas is gaining popularity in recent times due to its medicinal value but with less regards to its adverse health effect especially as it concerns male infertility. The current study was aimed at investigating the effect of Jatropha curcas seed extract on quality of semen and reproductive hormones in male Wistar rats. Methodology: Twenty-four male Wistar rats were randomly divided into four groups of six rats each. Group I served as control and was given 0.9% normal saline, while groups II, III and IV were administered 100, 200 and 400mg/kg of the extracts respectively for 60 days. The rats were then euthanised and blood samples collected via cardiac puncture for testosterone, LH and FSH assay; and semen collected for seminal fluid analysis. Results: There was significant (p<0.05) decrease in sperm count, significant (p<0.05) increase in total sperm abnormality, and significant (p<0.05) increase in sperm motility only at high dose when compared with the control. There was also significant (p<0.05) reduction in testosterone and FSH, while LH showed significant (p<0.05) increase. Conclusion: Jatropha curcas seed extract severed sperm quality with affectation of male reproductive hormones. Thus, male desirous of having babies should exercise caution in its patronage.Item Effect of prolonged heat exposure on red blood cell indices in-vivo among local bread bakers in Ilorin metropolitan city(Society of Basic and Applied Physiology, 2022) Ayinde, T.O.,; Oluwasola, A.,; Olohungbebe, K.A.,; Ojulari, L.S.,; Sanni, M.A.,; Afodun, A.M.,; Balogun, M.E.Background: Occupational hazards are specific dangers workers are exposed to, at work environments, due to tools, or interactions among co-workers. This is also seen among local bakers exposed to undue heat culminating into several unreported health conditions making the victims to indiscriminately and erroneously take blood supplements as antidotes. This study was conducted to explore the effect of prolonged environmental heat exposure on red blood cells indices in vivo among local bakers in Ilorin metropolitan city. Methodology: One hundred office workers and 100 local bread bakers with 5 years and above working exposure to prolonged heat were selected from 10 different local bakeries in Ilorin. Following completion of a questionnaire, blood samples were collected for assessment of red blood cell indices. Result: There was significant (p<0.05) increase in levels of haemoglobin, packed cell volume, and mean corpuscular haemoglobin, while there was no significant (p>0.05) change in the levels of RBC, MCV and MCHC, though there was increase. Conclusion: In conclusion, the result of this study demonstrated that prolonged heat exposure caused increase in the level of haemoglobin, packed cell volume, and mean corpuscular haemoglobin. However, red blood cell count, mean corpuscular volume, and mean corpuscular haemoglobin concentration of the bakery workers were uneventful compared to that of the control.Item Effect of prolonged heat exposure on red blood cell indices in-vivo among local bread bakers in Ilorin metropolitan city(Society of Basic and Applied Physiology, 2022) Ayinde, T.O.,; Olohungbebe, K.A., .; Ojulari, L.S.,; Sanni, M.A.,; Oluwasola, A.,; Afodun, A.M.,; Balogun, M.EBackground: Occupational hazards are specific dangers workers are exposed to, at work environments, due to tools, or interactions among co-workers. This is also seen among local bakers exposed to undue heat culminating into several unreported health conditions making the victims to indiscriminately and erroneously take blood supplements as antidotes. This study was conducted to explore the effect of prolonged environmental heat exposure on red blood cells indices in vivo among local bakers in Ilorin metropolitan city. Methodology: One hundred office workers and 100 local bread bakers with 5 years and above working exposure to prolonged heat were selected from 10 different local bakeries in Ilorin. Following completion of a questionnaire, blood samples were collected for assessment of red blood cell indices. Result: There was significant (p<0.05) increase in levels of haemoglobin, packed cell volume, and mean corpuscular haemoglobin, while there was no significant (p>0.05) change in the levels of RBC, MCV and MCHC, though there was increase. Conclusion: In conclusion, the result of this study demonstrated that prolonged heat exposure caused increase in the level of haemoglobin, packed cell volume, and mean corpuscular haemoglobin. However, red blood cell count, mean corpuscular volume, and mean corpuscular haemoglobin concentration of the bakery workers were uneventful compared to that of the control.Item Effect of testosterone supplementation on renal function in male albino rats.(Faculty of Basic Medical Sciences, Lagos State University College of Medicine, 2018) Ayinde, T.O.,; Olayaki L.A.,; Ojulari, L.S.,; Afodun, A.M.,; Quadri, K.K.; Fapounda, TObjectives: Testosterone is an anabolic steroid and it is secreted primarily by the testicles in males. Androgens have been shown to increase tubular sodium and water reabsorption and activate various vasoconstrictor systems in the kidneys. Testosterone increases blood pressure and may also influence renal electrolyte excretion. Methods: Fifteen adult male rats weighing 100 – 150g were randomly divided into three groups of five (5) rats each. Group 1 rats (Control) were given normal saline intramuscularly (IM). Group 2 rats were given 3mg/kg body weight (bw) of testosterone IM while Group 3 rats were given 9mg/kg bw testosterone IM for two weeks. All rats were allowed normal chow and water ad libitum. The animals were sacrificed under anaesthesia and blood samples collected through cardiac puncture. Results: Plasma urea levels of both low (2.02±0.23mmol/L) and high (10.3±0.61mmol/L) dose testosterone-treated animals were higher when compared with the Control group (1.94±0.07mmol/L), but the difference was only significant (p<0.05) in the high dose group. Asignificant (p<0.05) increase in plasma creatinine level in animals injected with low dose testosterone (33.0±2.46µmol/L) and high dose testosterone (46.0±5.67µmol/L) was observed when compared to the control group (25.2±3.91µmol/L). Conclusion: It was concluded from this study that exogenous administration of testosterone may alter normal kidney functions shown by elevated levels of urea and creatinine, but these could also be dose dependItem Hepatoprotective Effect of Tryptophan in Carbontetrachloride-Induced Hepatotoxicity in Male Wistar Rats.(Society of Basic and Applied Physiology, 2021) Ayinde, T.O.,; Olayaki, L.A.,; Ojulari, L.S.,; Oluwasola, A.,; Abdulraheem, H.A.,; Lawal, A.Z.,; Alli-Oluwafuyi, A.In the present study, tryptophan was evaluated for its hepatoprotective effects against carbontetrachloride-induced hepatocellular injury in rats. Hepatotoxicity was induced in male SpragueDawley rats by intraperitoneal injection of CCl4 (4ml/kg) in olive oil (1:1). Tryptophan at doses of 100mg/kg and 200mg/kg was administered orally for 28 days. The hepatoprotective effect of tryptophan was evaluated by the assay of biochemical parameters viz.: alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), alkaline phosphatase (ALP), total protein, albumin and lipid peroxidation. Tryptophan produced a dose-dependent significant increase (p<0.001) in serum ALP (41% & 60%), a dose-dependent decrease (p<0.001) in serum Malondialdehyde (61% & 65%), and a significant increase (p<0.001) in levels of serum protein and serum albumin, in CCl4induced hepatotoxic rats, following administration of 100 mg/kg bwand 200 mg/kg bw, respectively. The toxic effect of CCl4 in tryptophan treated groups was controlled significantly by restoration of the levels of enzymes, total protein and albumin as compared to the CCl4 treated groups. The results suggest that tryptophan is able to significantly alleviate the hepatotoxicity induced by CCl4 and may be attributed to the antioxidant property of tryptophan.Item Low glycaemic index foods, a better option for pre-exercise meals.(College of Medicine, Ambrose Alli University,, 2017) Ojulari, L.S.,; Ayinde, T.O.,; Kadir, R.E.,; Biliaminu, S.A.,; Nafiu, A.B.,; Ayinla, M.T.,; Jimoh-Abdulghaffaar, H.O.,; Imam-Fulani, A.O.; Fatai, R.A.Item Nephroprotective Effects of Anona Muricata Leaf Extract on Adenine-Induced Renal Failure in Male Wistar Rats.(Society for Conservation of Phytofuels and Sciences., 2021) Ayinde, T.O.,; Ojulari, L.S.,; Afodun, A.M.,; Oluwasola A.,; Balogun M.E.,; Olanrewaju M.O.; Tajudeen O.A.,; Faramade A.A.Renal failure is a prevalent disease in Africa, partly as a result of indiscriminate consumption of various medical herb preparations such as Annona muricata, which is locally used for several ailments like inflammation, bacterial and parasitic infections, diabetes, liver diseases amongst others. The present study was aimed at investigating the effect of Annona muricata leaf (AML) extract on adenine-induced renal failure in male Wistar rats. Thirty-five rats were divided into five groups, with seven rats per group. Each of the rats in groups II-V were given 50mg/kg adenine for induction of renal failure for a period of 14 days. The rats in groups III V were treated with 50mg/kg, 100mg/kg and 200mg/kg of the extract respectively following induction of renal failure, while group II was left untreated for a period of 28 days totaling 42 days all together. Rats in group I served as Control. Urinalysis was done prior to the end of the experiment. The animals were euthanised and blood samples collected for assessment of haematocrit, plasma electrolytes, urea and creatinine. Significant sodium reduction was observed in co-administration of 50mg AML and 50mg adenine, and 50mg adenine alone. Significantly increased potassium was noticed in co-administration 50mg AML and 50mg adenine, and 50mg adenine alone. Significant increase was noted in both urea and creatinine levels of co-administration of 50mg or 100mg AML and 50mg adenine. Haematocrit was only significantly increased in the 200mg AML administration group. There was global proteinuria in all AML intervention groups which was seemingly dose dependent.AML, if administered at a high dose has a nephron-protective effect against acute renal failure perhaps due to modulation in some ion channels in renal tubules.