Browsing by Author "Atolani, O"
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Item Antioxidant and cytotoxicity of β-Amyrin acetate fraction from Bridellia ferruginea Leaves.(Asian Pacific Journal of Tropical Biomedicine. Published by Elsevier, 2012) Fabiyi, O.A; Atolani, O; Adeyemi, O.S; Olatunji, G.AObjective: The objective of this work was to determine the beta-amyrin acetate fraction in leave extract of Bridelia ferruginea and evaluate for its antioxidant and cytotoxicity potentials. Methods: The dried and pulverized leaves of Bridelia ferruginea was extracted with hexane and then with ethyl acetate. The concentrated ethylacetate extract subjected to silica gel column chromatography and eluted with a mixture of equal volume of hexane and dichloromethane afforded two major fractions. The more polar fraction was concentrated and subjected to GCMS analysis which afforded the steroid, 12-Oleanen-3yl acetate commonly known as beta-amyrin acetate (66.14%). Its ability to act as a scavenger of DPPH radical and its cytotoxicity potential based on brine shrimp assay were investigated. Results: The DPPH antioxidant assay revealed that the fraction had a higher antioxidant potential with an IC50 value of 158.2毺g/mL relative to gallic acid which had IC50 of 201.1 毺g/mL. The cytotoxicity assay using the brine shrimp a gave LC 50 values of 319 and 5.86 毺g/mL for acute and lethal doses respectively indicating extreme toxicity when compared to reference drug, cyclophosphamide which had LC50 value of 2506 毺 g/mL. Conclusions: Thus, the beta-amyrin acetate has been identified for the first time in the leave of Bridelia ferruginea. The data here suggest that the beta-amyrin acetate fraction of the leave of Bridelia ferruginea could be further explored in biological profiling requiring antioxidant and cytotoxic dependent therapeutics as the plant could be a viable source of antioxidant and cytotoxic agents in cancer chemotherapy in the near futureItem Blighia sapida; The Plant and Its Hypoglycins an Overview.(Journal of Scientific Research ,Institute of Chemistry, University of The Punjabi Pakistan, 2009) Atolani, O; Olatunji, G.A; Fabiyi, O.ABlighia sapida g; family Sapindaceae is a multi purpose medicinal plant popular in the western Africa. It is well known for its food value and its poisonous chemical contents being hypoglycins A& B (unusual amino acids.) The hypoglycin Ais more available in the fruit than hypoglycin B. Hypoglycin A have been used as glucose inhibitor therapy, thereby giving room for the plant to be used for orthodox medicinal purposes in future. Its other therapeutic values have been reported as well. The ingestion of hypoglycin A forms a metabolite called methylenecyclopropane acetyl CoA (MCPACoA) which inhibit several enzymes A dehydrogenases which are essential for gluconeogenesis. This review covers history, description, origin and uses of Blighia sapida with emphasy on the fruit and its associated biologically active component (hypoglycins) and tries to show why the plant can be used as the sources of many potential drugs in treatment of diseases, especially glucose related ones. The mechanism of hypoglycin A metabolism is also explained. The hypoglycin A potential glucosesuppressing activities warranted further studies for the development of new anti-diabetes drugs with improved therapeutic valuesItem Chemical characterization, antioxidant, cytotoxicity, anti-toxoplasma gondii and antimicrobial potential of the Citrus sinensis seed oil for sustainable cosmeceutical production.(Heliyon Elsevier, 2020) Atolani, O; Adamu, N; Oguntoye, O.S; Zubair, M.F; Fabiyi, O.A; Oyegoke, R.A; Adeyemi, O.S; Areh, E.T; Tarigha, D.E; Kambizi, L; Olatunji, G.AThere are growing concerns about the chronic and acute effects of synthetic additives such as antibacterial, fragrances, colourants and stabilizing agents used in the production of various household products. Many household products and materials including cosmetic products are reportedly suspected to be carcinogenic with some acting as endocrine disruptors among other effects. Thus, environmental-friendly alternatives such as products that are rich in bioactive phytochemicals are becoming consumers' preferred choice especially in the beauty and cosmetic sector. ‘Green’ preparation of medicinal soaps devoid of any synthetic additives was made from underutilized tropical seed of Citrus sinensis seed oil and some natural additives comprising of natural honey, Ocimum gratissimum leaves extract, Moringa oleifera seed oil and coconut oil. Precisely, the seed oil of the underexplored C. sinensis was obtained via soxhlet extraction and saponified with natural lye solution at different ratios to produce soaps of varying characteristics. The incorporation of honey and Ocimum gratissimum leaf extract provided additional antimicrobial, antioxidant and fragrance properties. Physico-chemical parameters of the oil and soaps were determined following standard procedures while the fatty acid profile of the trans-esterified oil was determined using GC–MS. The antimicrobial potential of the oil and soaps were assessed using agar diffusion method at concentrations 200 mg/mL and below. Linoleic acid (36%) and oleic acid (27%) were the most prominent in C. sinensis seed oil. The soap had antimicrobial potential comparable to commercial product. The soap samples recorded highest anti-bacteria activities (22.0 1.0–23.0 1.0) against Staphylococcus aureus and Bacillus subtilis and notable anti-fungi activities (18.0 1.0) against Penicillium notatum and Candida albicans. Additionally, the oil showed moderate anti-parasite (anit-toxoplasma gondii) activity (EC50 500 μg/mL) but with improved selectivity that precludes oxidative stress while the prepared medicinal soaps exhibited remarkable antioxidant property. The utilization of these locally sourced resources will prevent the daily introduction of synthetic antimicrobial and antioxidant chemicals into the environment. The initiative avail a sustainable production of environmentally-benign cosmetic products besides conversion of waste to wealth agrees which aligns with the Sustainable Development Goals (SDGs).Item Chemical composition, antioxidant, anti-lipooxygenase, antimicrobial, anti-parasite and cytotoxicity of Polyalthia longifolia seed oil.(Medicinal Chemistry Research Springer nature, 2019) Atolani, O; Areh, E.T; Oguntoye, O.S; Zubair, M.F; Fabiyi, O.A; Oyegoke, R.A; Tarigha, D.E; Adamu, N; Adeyemi, O.S; Kambizi, L; Olatunji, G.AThis work investigates the chemical compositions of Polyalthia longifolia Thw. seed oil with the associated antioxidant, anti-inflammatory, anti-parasite and cytotoxicity potentials. The oil of P. longifolia seed obtained by soxhlet extraction was trans-esterified and the fatty acid profile characterized using gas chromatography mass spectrometry (GC–MS). The antioxidant activity was evaluated using DPPH and ABTS assays. The anti-bacterial and anti-fungi properties of the oil were determine on clinical isolates of the organisms using agar diffusion method. The anti-inflammatory activities, cytotoxicity and anti-parasite potential were evaluated using lipooxygenase, mammalian cell and Toxoplasma gondii assays respectively. P. longifolia seed was observed to contain oleic (30.31%), linoleic acid (19.27%) and palmitic acid (15.11%) as the major fatty acids with low proportion of tricosylic acid (6.10) and stearic acid (5.56%). The oil had significant anti-lipooxygenase activity (IC50 = 0.70 ± 0.02 µg/mL) comparable to indomethacin (IC50 = 0.53 ± 0.07 µg/mL). The DPPH (IC50 = 55.91 ± 31.18 µg/mL) and ABTS (IC50 = 16.89 ± 15.50 µg/mL) antioxidant activity of the oil was lower to the ascorbic acid (IC50 = 0.34 ± 0.04 and 0.54 ± 0.04 µg/mL). The oil also showed activities against all the tested bacteria and fungi. The highest inhibition was recorded against S. aureus (17 ± 1 mm) at concentration 200 mg/mL. Further, the oils showed strong potential to restrict growth of Toxoplasma gondii in vitro, but the parasite growth inhibition was mildly abated in the presence of α- tocopherol. The seed oil of the underutilized P. longifolia possesses essential fatty acids which could be responsible for the numerous biological potentials which include anti-lipooxygenase, antioxidant, anti-inflammatory, anti-parasite, antimicrobial and cytotoxic activities. The incorporation of the natural oil into pharmaceuticals or cosmetics may enhance antioxidant, anti-inflammatory, antimicrobial and cytotoxicity potential of such products.Item ). Effect of thermal degraded products of Cymbopogon citratus on the in vitro survival of Meloidogyne incognita eggs and juveniles.(Ceylon Journal of Science. University of Peradeniya, Sri Lanka., 2018) Fabiyi, O.A; Olatunji, G.A; Adebayo, M.O; Atolani, OThe products of thermally-degraded Cymbopogon citratus were evaluated for nematicidal activity as an alternative of toxic synthetics. The products exhibited moderate nematicidal activity, but not as significantly (p<0.05) effective as carbofuran, a synthetic nematicide. Thermally degraded products of fresh Cymbopogon citratus (CMGC/th/fresh) was the most promising at 90 mg/mL and could be used in place of the toxic synthetic nematicideItem Identification of some chemical constituents of the leaves of Alstonia boonei and Bridelia ferruginea(Selcuk Journal of Agriculture and Food Science. Selcuk University, 2016) Fabiyi, O.A; Atolani, O; Olatunji, G.AThis study aims to investigate the phytochemical composition of the leaves of Alstonia boonei and Bridelia ferruginea. The leaves of the two plants were extracted successively with n-hexane and ethyl acetate, concentrated and fractionated over gravity silica gel column chromatography. Some fractions obtained were analyzed using gas chromatography mass spectroscopy (GC-MS). GC-MS results indicated that the main constituents of the fractions from Alstonia boonei were fatty acid methyl esters, while the main constituents of the fractions from Bridelia ferruginea were fatty acid esters (47.46%) and triterpenoids (43.39%). Each of the fractions has at least one exclusive tetracyclic or pentacyclic triterpenoid present. Friedelan-3-one and clionasterol were present in the Alstonia boonei fractions, while beta-amyrin, lup-20(29)-en-3-one, lupeol, alpha-amyrin acetate and 4,4,6a,6b,8a,11,11,14b-octamethyl-1,4,4a,5,6,6a, 6b,7,8,8a,9,10,11, 12,12a,14,14a,-octadecahydro-2H-picen-3-one were present in the B. ferruginea fraction. Obviously, B. ferruginea is a reservoir to many pentacyclic triterpenoids. The pentacyclic triterpenoids may be used as a biomarker in the chemo taxonomical classification of the plants whilst acting as relevant contributors to the acclaimed biological benefits of B. ferruginea reported in traditional medical practices. The leaves of the two plants contain many bioactive compounds which may find application in pharmaceutical formulations as well as nutraceuticals and bio-pesticides preparations.Item Isovitexin from Kigelia pinnata, A Potential Eco-friendly Nematicidal Agent.(Tropical Agriculture. University of West Indies, Trinidad, 2014) Atolani, O; Fabiyi, O.A; Olatunji, G.ASynthetic pesticides are usually environmentally hazardous. In an attempt to obtain an eco-friendly alternative to synthetic nematicidal agents, isovitexin, a flavonoid glycoside isolated from butanol fraction of the leaf extract of Kigelia pinnata was examined for its in vitro nematicidal potential. The chemical structure of the isovitexin was confirmed by comparing its UV, IR, MS and NMR spectroscopic data with that reported in literature. The compound induced a hundred percent mortality at 24 h of exposure and inhibited egg hatch of Meloidogyne incognita at a concentration of 0.16 mg/mL. There was no significant difference (p<0.05) in the action of isovitexin and the synthetic nematicide, oxamyl, when tested at the same concentration. The bioactivity and toxicity evaluation on ‘molinspiration’ and ‘toxpredict’ evaluators indicated that isovitexin is a potentially bioactive compound with low genotoxic carcinogenicity and high enzyme inhibition activity. The biodegradability evaluation indicated that it belongs to a class 2 persistent chemical. The results of this study could be harnessed to make isovitexin commercially available as a natural nematicide. This is the first account of isovitexin being isolated from Kigelia pinnata and evaluated for nematicidal potential.Item Lawsonia inermis in the control of Meloidogyne spp on Corchorus olitorius.(Electronic Journal of Environmental, Agricultural and Food Chemistry. Universidad de Vigo, Spain, 2011) Fabiyi, O.A; Atolani, OThe efficacy of cypress shrub, Lawsonia inermis L. in the control of root – knot nematodes, Meloidogyne spp was investigated in the laboratory and the Screenhouse. In the laboratory, only the aqueous extract of the test plant (Lawsonia inermis) was assessed for its effects on nematode egg hatch and juvenile mortality. For the Screenhouse, the experimental design was a 2x2x3 factorial experiment, fitted into a randomized complete block design, involving two levels of nematode population (0 and 300) and two forms of test plant application (aqueous extract and powdered form). In the laboratory, the aqueous extracts of Lawsonia inermis caused a significant reduction (P< 0.05) in nematode egg hatched. Egg hatch was 92.0% in the untreated control (0% extract) as compared to 11.7% in 25% crude extract of Lawsonia inermis (L) extract. Within two days of the experiment 98.4% mortality was observed in 100% crude concentration of Lawsonia inermis (L), as compared with 0% mortality in the control. All concentrations of the extract caused significantly higher mortality than the control. In the screenhouse, 15% aqueous crude concentration, significantly reduced nematode population in the root and in the soil. All treated plants were also less galled compared to the untreated control.Item Natural antifungal compounds from the peels of Ipomoea batatas Lam(Taylor and Francis, 2015) Oluyori, A.P; Shaw, A.K; Preeti, R; Reddy, S; Atolani, O; Olatunji, G.A; Fabiyi, O.AThree antifungal compounds have been isolated for the first time from the peels of Ipomoea batatas Lam. Their structures were established on the basis of 1D and 2D NMR spectra data as well as ESI-MS and IR analysis. Urs-13(18)-ene-3β-yl acetate was found to possess a weak activity against Sporothrix schenckii and Trichophyton metagrophytes fungi with an MIC value of 50 μg/mL each. Stigmasterol and 3-friedelanol were equally active against T. metagrophytes.Item Nematicidal activities of Chromatographic Fraction from Alstonia boonei and Bridelia ferruginea on Meloidogyne incognita.(Pakistan Journal of Nematology. Pakistan Nematological Society, 2012) Fabiyi, O.A; Olatunji, G.A; Atolani, OThe leaves of Alstonia boonei (de Wild) and Bridelia ferruginea (Benth) were air dried at ambient temperature (27ºC) and subjected to successive cold extraction using n-Hexane, Ethyl acetate and Ethanol. Thirty grams from the resulting crude extracts were further subjected to open column chromatography on silica gel (100-120 mesh grades) using glass column. The chromatographic fractions were tested in vitro along with their crudes and carbofuran on Meloidogyne incognita juveniles and eggs. The fractions were significantly effective in causing juvenile mortality. 75% concentrations of fraction being more active and showed significant difference from other concentrations. Fractions from A. boonei were significantly (p<0.05) effective in the first hour of exposure to M. incognita juveniles with a percentage mortality of 48.62% which was not significantly different from carbofuran. At 4th and 6th hour of juvenile exposure to treatment, fractions from A. boonei were significantly (p<0.05) more effective than carbofuran with a percentage mortality of 67.87 and 72.57% against carbofuran with 63.77 and 69.31% mortality, respectively. Mortality increased with increase in exposure time. The fractions were also as effective as carbofuran in inhibiting egg hatch, but there was minimum inhibition among the crude extracts. Spectroscopic analyses revealed that the fractions contain organic compounds that are nematicidal in nature which include phenols, carboxylic acids, aliphatic hydrocarbons, aldehydes, anhydrides, amides, mono and di substituted aromatics and long chain carbon fatty acid estersItem Nematicidal Isochromane Glycoside from Kigelia pinnata leaves.(Acta Agriculturae Slovenica. University of Ljubljana, Slovenia, 2014) Atolani, O; Fabiyi, O.A; Olatunji, G.ASynthetic nematicides such as oxamyl and carbofuran play significant roles in the management of plant-parasitic nematodes. However, their negative environmental impacts have it imperative to search for safer alternatives. As part of our contribution in the search for bio-nematicides, compounds from plant extract were screened for possible potent nematicidal agent. A new isochromane carboxylic acid glycoside, isolated from the leaves of Kigelia pinnata (Lam.) Benth (Bignoniaceae) was evaluated for its nematicidal activity. The structure of the proposed compound was characterized by various spectroscopic methods, which included UV, FTIR, 1D-, and 2D-NMR, FAB-MS, TOF-ESI-MS and TOF-ESI-MS/MS (TANDEM). The in vitro experiment conducted on the glycoside against Meloidogyne incognita juveniles and eggs indicated an induced mortality. Its activity can be compared favourably with oxamyl, when tested at 0.1 mg/mL concentration. At four hours of observation, no significant difference (P < 0.05) between oxamyl and the glycoside was observed. The present data sustains that natural glycoside is a promising oxamyl alternate for controlling nematode-induced plant root knots and may contribute to integrated pest management.Item Phytochemicals from Kigelia pinnata leaves shows antioxidant and anti-cancer potentials on human cancer cell line.(Journal of Medicinal Food., 2031) Atolani, O; Olatunji, G.A; Fabiyi, O.A; Adekunle, A.J; Ogbole, O.OStudies suggest that the traditional applications of Kigelia pinnata leaves have beneficial effects against oxidative stress–mediated diseases and cancers. The pulverized dried leaves of K. pinnata were extracted with hexane, ethyl acetate, and methanol sequentially, and the crude extracts were fractionated by silica gel column chromatography with solvent gradient of increasing polarity. 3-hydro-4,8-phytene, trans-phytol, (9Z,12Z)-methyl octadeca-9,12-dienoate, and two oil fractions were obtained. The chemical compositions of chromatographic fractions were determined using gas chromatography–mass spectroscopy. The structure elucidations of the isolated compounds were based on FTIR, MS, and NMR spectral data analyses. These along with the crude extracts were examined for their antioxidant activities using ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS) assays. Total phenolic contents were also determined. The crude extracts and purified compounds were evaluated on the rhabdomyosarcoma human cancer cell for their cytotoxicity using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assays. The methanol extract was richer in phenolics and was most potent as antioxidant and cytotoxic agent among all the substances tested. Among the fractions and pure compounds, the two oil fractions showed more cytotoxicity potency, with IC50s of 143.4 – 0.5 and 147.9 – 1.3 ng/mL, which is more significant than the reference standard,cyclophosphamide (165.6 – 1.0 ng/mL). 3-hydro-4,8-phytene showed lower antioxidant and cytotoxicity potential (IC50 = 1818 – 5.2 lg/mL and 171.7 – 0.8 ng/mL, respectively). Trans-phytol did not show a high cytotoxic power (IC50 =769.8 – 4.3 ng/mL). The comparatively high cytotoxicity index of (9Z, 12Z)-methyl octadeca-9,12-dienoate (IC50 = 153.3 –0.1 ng/mL) indicated that it may be one of the principal cytotoxic agent in the ethyl acetate extract. These results suggest that the leaves of K. pinnata possess tumor cytotoxic potential and could be part of a drug combination for future cancer therapyItem Plant Parasitic Nematodes Management Through Natural Products: Current Progress and Challenges.(Springer Nature, 2020) Atolani, O; Fabiyi, O.AThe devastating effect of huge crop losses to field and storage pest brings about a downturn in the availability of food, thus subjecting the citizens to poverty and hunger. The plant-parasitic nematodes (PPN) are important agricultural pests, which are hidden enemies of the farmers. Their existence in soil and the gravity of the potential loss which accrues to them are not known to the lay farmers. Significant quantitative and qualitative economic losses are attributed to their infestation worldwide on a yearly basis. The damage posed by plant-parasitic nematodes is steadily on the increase and is at times not reported, but oftentimes associated with factors such as nutrient deficiency and extreme weather conditions. Nematodes are a big challenge to food production in an overpopulated world. They are listed as an important crop pest in the world, largely because of their wide geographical distribution and their ability to parasitize virtually all crops. Export trade restrictions are often imposed due to the presence of quarantine nematode pests. Different integrated pest management (IPM) strategies have been employed in the control of plantparasitic nematodes with various degrees of success. The use of synthetic nematicides, though an established internationally recognized effective approach, is largely negated by the toxicity and adverse effects on humans and the environment. Thus, the need for concerted worldwide intensive research for safer alternatives has become imperative. However, there are several challenges in the control of plantparasitic nematodes, majorly the adoption of new approach to control by the farmers, large-scale production hiccups, government policies, and potential toxicity of the new approach. This work reviews the various strategies used in combating plantparasitic nematodes. Based on the resultant toxicities reported for chemical control and the effectiveness of biological control (bio-control) which is considered safer, more discussions were made on bio-control. Biological control, application of botanicals, and soil amendment methods rank high among others owing to their environmental safety. Over 2400 plant species worldwide are known to be pesticidal. Some tested plants and phytochemical isolates with reported in vitro or in vivo nematicidal properties were listed. The merits and demerits of the bio-control method were also discussed. It was suggested that although the bio-control method is affordable and effective, it should be performed with caution as many emerging reports indicated that many plant materials contain phytochemicals that could induce carcinogenicity, endocrine disruption, and various degrees of toxicities with endpoints such as hepatotoxicity, cytotoxicity, ecotoxicity, carcinogenicity, mutagenicity, and immunotoxicity which finally result into morbidity and mortalityItem Preparation of bio-nematicidal nanoparticles of Eucalyptus officinalis for the control of cyst nematode (Heterodera sacchari).(The Journal of Animal and Plant Science. Pakistan Agricultural Society, 2020) Fabiyi, O.A; Olatunji, G.A; Atolani, O; Olawuyi, R.OThe production of Oryza sativa (rice) which is one of the most important food crops is greatly limited by nematode infestations. While various integrated control measure which include the application of synthetic nematicidal agents have been adopted, success without some major setback has been elusive. Thus, the need for concerted worldwide intensive research for safer alternatives has become imperative. Silver nanoparticles (NPs) of Eucalyptus officinallis were prepared via green synthesis approach. The nanoparticles obtained were subjected to UV-Vis and FT-IR spectroscopies for the absorption and functional groups characterisations respectively. Surface Plasmon Resonance (SPR) was achieved at 451nm, indicating the formation of NPs and the FTIR showed functional groups which further confirmed the formation of nanoparticles. The NPs significantly (p=0.05) reduced nematode population in root and soil, increased vegetative growth of rice plant, with a significant corresponding increase in yield. The plant-based nematicidal agent of silver nanoparticles origin cheaply prepared in this study adds credence to claim that bio-nematicides holds promise as a costeffective, efficient and eco-friendly option for the future.Item Proteinease Inhibitory, Membrane Stabilization, Phytochemical and Antioxidant Evaluations of Leaves, Seeds and Calyces of Four Selected Edible Medicinal Plants.(Cogent Chemistry (Taylor and Francis Group), 2017) Kambizi, L; Bakare-Odunola, M. T.; Oladiji, A. T.; Kola-Mustapha, A. T.; Amusa, T. O.; Atolani, O; Njinga, N. S.; Quadri, A. L.The aqueous extracts of the edible parts of four common medicinal plants (Hibiscus sabdariffa, Telfairia occidentalis, Moringa oleifera and Ocimum gratissimum) readily consumed as food or supplements in many African settings are examined for some of their acclaimed pharmacological activities and possible potential associated risks. The classes of phytochemicals present are examined qualitatively and quantitatively, while the antioxidant activities were determined using three assays which include 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS) and hydrogen peroxide (H2O2) assays. The anti-inflammatory activities were also examined using proteinase inhibitory assays and red blood cell membrane stabilisation assays. Both the antioxidant and anti-inflammatory activities of the plants indicated that the plants have potentials for applications in foods and nutraceuticals that can be used for the management of various ailments and diseases. However, the consumption of H. sabdariffa leaf should be highly regulated as the extract exhibited reduced cell membrane stabilisation activities at increased concentrations.Item Toxicity Effect of Eucalyptus globulus on Pratylenchus spp of Zea mays.(Sarhad Journal of Agriculture. University of Agriculture, Peshawar Pakistan, 2020) Fabiyi, O.A; Atolani, O; Olatunji, G.AMaize (Zea mays L.) is an important food crop widely consumed in various forms in many parts of the world. Its annual yield is largely hampered by nematode infestation, especially the Pratylenchus spp. Experiments were conducted in a screenhouse and under field conditions to evaluate the toxicity of fractions and essential oil of Eucalyptus globulus (Myrtaceae) against Pratylenchus spp. infecting Zea mays. The phytochemical basis of the plant was partly established using Infrared (IR) spectroscopy, Gas Chromatography – Mass Spectrometry (GC-MS), proton and carbon-13 Nuclear Magnetic Resonance (1H-NMR and 13C NMR) Spectroscopy analysis. Effect of application of plant phytochemicals on the planted maize was also examined in terms of growth rate and survival of Pratylenchus spp. The essential oil (ECSG/EO) exhibited significant (p<0.05) toxicity, reduced nematode population and increased grain yield (6.79 kg) compared to the standard, carbofuran (CBFN) (7.18 kg) The Eucalyptus globulus ethanol extract fractions (ECSG/ EtOH) showed moderate effect, while the chopped leaf materials used as soil amendment (ECSG/AMDM) exhibited the least potency among all the treatments. Spectroscopic analysis by GCMS revealed the presence of terpenoids and phenolics as major constituents of E. globulus. The essential oil showed the presence of 1,8-cineole (23.3%), citronella (18.1%), geranial (17.6%), isopulegol (10.4%), myrcene (13.0%), cuminaldehyde (9.1%) and 2-pinene (8.5%) as major constituent. Results obtained in this research revealed that essential oil from Eucalyptus (a bio-nematicide) holds promise as an affordable and environmentally-friendly alternative for nematode control and increased crop yield particularly in maize production