Nematicidal activities of Chromatographic Fraction from Alstonia boonei and Bridelia ferruginea on Meloidogyne incognita.
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Date
2012
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Pakistan Journal of Nematology. Pakistan Nematological Society
Abstract
The leaves of Alstonia boonei (de Wild) and Bridelia ferruginea (Benth) were air dried at
ambient temperature (27ÂșC) and subjected to successive cold extraction using n-Hexane, Ethyl
acetate and Ethanol. Thirty grams from the resulting crude extracts were further subjected to open
column chromatography on silica gel (100-120 mesh grades) using glass column. The
chromatographic fractions were tested in vitro along with their crudes and carbofuran on
Meloidogyne incognita juveniles and eggs. The fractions were significantly effective in causing
juvenile mortality. 75% concentrations of fraction being more active and showed significant
difference from other concentrations. Fractions from A. boonei were significantly (p<0.05)
effective in the first hour of exposure to M. incognita juveniles with a percentage mortality of
48.62% which was not significantly different from carbofuran. At 4th and 6th hour of juvenile
exposure to treatment, fractions from A. boonei were significantly (p<0.05) more effective than
carbofuran with a percentage mortality of 67.87 and 72.57% against carbofuran with 63.77 and
69.31% mortality, respectively. Mortality increased with increase in exposure time. The fractions
were also as effective as carbofuran in inhibiting egg hatch, but there was minimum inhibition
among the crude extracts. Spectroscopic analyses revealed that the fractions contain organic
compounds that are nematicidal in nature which include phenols, carboxylic acids, aliphatic
hydrocarbons, aldehydes, anhydrides, amides, mono and di substituted aromatics and long chain
carbon fatty acid esters
Description
Article on Nematode Control
Keywords
Alstonia boonei; Bridelia ferruginea; chromatographic fractions; Meloidogyne incognita
Citation
Fabiyi, O.A., Olatunji, G.A and Atolani, O. (2012). Nematicidal activities of Chromatographic Fraction from Alstonia boonei and Bridelia ferruginea on Meloidogyne incognita. Pakistan Journal of Nematology 30 (2): 189-198.