Antioxidant and cytotoxicity of β-Amyrin acetate fraction from Bridellia ferruginea Leaves.
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Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
Asian Pacific Journal of Tropical Biomedicine. Published by Elsevier
Abstract
Objective: The objective of this work was to determine the beta-amyrin acetate fraction in
leave extract of Bridelia ferruginea and evaluate for its antioxidant and cytotoxicity potentials.
Methods: The dried and pulverized leaves of Bridelia ferruginea was extracted with hexane
and then with ethyl acetate. The concentrated ethylacetate extract subjected to silica gel column
chromatography and eluted with a mixture of equal volume of hexane and dichloromethane
afforded two major fractions. The more polar fraction was concentrated and subjected to GCMS
analysis which afforded the steroid, 12-Oleanen-3yl acetate commonly known as beta-amyrin
acetate (66.14%). Its ability to act as a scavenger of DPPH radical and its cytotoxicity potential
based on brine shrimp assay were investigated. Results: The DPPH antioxidant assay revealed
that the fraction had a higher antioxidant potential with an IC50 value of 158.2毺g/mL relative to
gallic acid which had IC50 of 201.1 毺g/mL. The cytotoxicity assay using the brine shrimp a gave
LC
50 values of 319 and 5.86 毺g/mL for acute and lethal doses respectively indicating extreme
toxicity when compared to reference drug, cyclophosphamide which had LC50 value of 2506 毺
g/mL. Conclusions: Thus, the beta-amyrin acetate has been identified for the first time in the
leave of Bridelia ferruginea. The data here suggest that the beta-amyrin acetate fraction of the
leave of Bridelia ferruginea could be further explored in biological profiling requiring antioxidant
and cytotoxic dependent therapeutics as the plant could be a viable source of antioxidant and
cytotoxic agents in cancer chemotherapy in the near future
Description
Antioxidant and Cytotoxicity article
Keywords
Brine Shrimp; beta-amyrin acetate;12-Oleanen-3yl acetate; steroid; antioxidant
Citation
Fabiyi, O.A., Olubunmi Atolani, Oluyomi S Adeyemi and Gabriel Ademola Olatunji (2012). Antioxidant and cytotoxicity of β-Amyrin acetate fraction from Bridellia ferruginea Leaves. Journal of Tropical Biomedicine. S981-984.