Browsing by Author "Ayinde, T.O."
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Item A Comparative Study on the Antioxidant Potentials of Honey and Some Selected Beverages in Male Wistar Rats.(Faculty of Education, Al-Hikmah University, 2019) Oluwasola, A.; Ayinde, T.O.This study compared the antioxidant potentials of honey and some selected beverages in male wistar rats. Twenty five male wistar rats (220-240g) were assigned into 5 groups of 5 animals each, such that the rats in groups I, II, III, IV and V received orally 1mL distilled water, 0.2mg/kg body weight (BW) of honey, 0.2mg/kg BW of vitamin C, 0.3mg/kg body weight (BW) of zobo and 0.2mg/kg BW of cocoa power, respectively. Catalase (WBC), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured using standard methods. There was significant (p<0.05) increase in catalase and superoxide dismutase (SOD) levels for all the treatment groups when compared with the control. The order of significant (p<0.05) increased in these two parameters among the treatment groups were: cocoa powder < vitamin c < zobo < honey respectively. However, there was significant (p<0.05) decrease in malondihydehyde (MDA) levels for all the treatment groups when compared with the control. The order of significant (p<0.05) decreased in MDA levels among the treatment groups were: honey < zobo < vitamin c < cocoa powder respectively. This study showed that honey, zobo, vitamin c and cocoa powder could stimulate the activities of antioxidant enzymes by preventing the formation of lipid peroxidation. The consumption of honey, zobo, vitamin c and cocoa powder are therefore recommended in order to potentiate the activities of antioxidant enzymes. In addition, honey has the highest antioxidant potential and could consume more than cocoa powder which has the lowest.Item A new model for alloxan-induced diabetes mellitus in rats.(Journal of Bangladesh Society of Physiologists, 2019) Ojulari, L.S.; Oladeru, O.O.,; Ayinde, T.O.; Alade I.O.; Kadir, R.E.; Dangana, O.E.Background:Alloxan is widely used to induce experimental diabetes mellitus (DM) in animals with different grades of disease severity by varying the dose of Alloxan used. This method has however be questioned by recent research work as an appropriate technique for the induction of diabetes. Objective: To provide a simple, yet concise and reproducible experimental procedure and model for Alloxan-induced DM in rats. Methods: The study was divided into 2 separate experiments. Experiment 1: Alloxan was administered, into four subgroups each (group 1- 100 mg of Alloxan /kg of rat body weight, group 2- 120 mg/kg, group 3- 150 mg/kg, and group 4- 170 mg/kg); in each subgroup, the dose of Alloxan was administered at different concentrations (20 mg/ml, 10 mg/ml, 5 mg/ml and 4 mg/ml) in groups of 10 rats each. The pre-induction fasting period was also varied between groups. Experiment 2:Following a pre-induction fasting period of 36 hours, animals received 150 mg Alloxan /kg body weight and at a concentration of 20 mg Alloxan/ ml. Result:Alloxan administered intraperitoneally at 150 mg/kg of rat body weight, at 20 mg/ml and following a pre-induction fast period of 36 hours yielded the most favorably conditions with the least recorded mortality. Conclusion: From the results of this study, it can be concluded that alloxan is a diabetogenic drug with a strict protocol of use in inducing a predictable DM in rats and as such, this model is a standard and reproducible technique for the induction of DM in experimental rats.Item Androgenic Effects of Aqueous seed extract of Moringa oleifera in Male Wistar Rats(Published by School of Medicine and Health Sciences, University of Papua, New Guinea, 2022) Ayinla M.T; Muhammad, A.S.; Ayinde, T.O.; Ojulari, L.S.; Owoyele, B.V.; Asuku, A.O.; Adebisi, R.O.; Badmus, O.A.; Krishnamurthy, R.Androgenic effects of Aqueous Seed-extract of Moringa oleifera (ASMO) in male Wistar rats were investigated. Eighteen (18) male Wistar rats weighing 200-240g were used for this study. The rats were divided into three (3) groups: Control (Group 1) that received 10 ml/kg of normal saline, Group 2: received low dose of ASMO (200mg/kg), and Group 3: received high dose of ASMO (500mg/kg). The animals were treated for twenty-eight days. On the 29th day, the rats were sacrificed and the testes were carefully removed for semen and biochemical analysis. Body weight, reproductive and vital organ weights were determined. Sperm parameters (motility, morphology, count and viability), tissue testosterone, luteinizing hormone (LH), Malondialdehyde (MDA) and Catalase were also determined using standard methods. Data were analysed using one-way ANOVA followed by Duncan new multiple range post hoc test. The result showed ASMO significantly increased (p<0.05) the final body weight, weight of reproductive and vital organs. Moreover, 200mg/kg body weight dose of ASMO significantly increased (p<0.05) the sperm parameters but 500mg/kg body weight dose significantly decreased it. ASMO also caused a significant dose dependent increase (p<0.05) in testosterone and catalase level but a significant decrease (p<0.05) in MDA and LH level compared with the control. In conclusion, this study revealed that ASMO has androgenic effects in male rats and thus provides a basis for the traditional use of Moringa oleifera in the management of male sexual disorders.Item COMPARATIVE ANALYSIS OF HANDGRIP STRENGTH AND URINE C-PEPTIDE CREATININE RATIO AS BIOMARKERS FOR GLUCOSE REGULATION IN YOUNG ADULT FEMALES IN THE UNIVERSITY OF ILORIN: A CROSS-SECTIONAL STUDY(Society for Experimental Biology of Nigeria, 2023) Ojulari, L.S.; Sulaiman, S.E.; Ayinde, T.O.; Kadir, R.E.; Hidaayah O. Jimoh-Abdulghaffaar, H.O.; Sulaiman, H.Handgrip strength (HGS) is a robust biomarker predicting future disability, metabolic syndrome, and diabetes. Urinary C-peptide creatinine ratio (UCPCR) emerges as a novel, non-invasive tool under exploration for assessing beta cell function and glucose regulation. Despite their significance in gauging muscle strength, mass, and overall metabolic function, gaps remain in understanding the full extent of handgrip strength and UCPCR's efficiency. This study aimed to identify a better biomarker for glucose regulation by studying the relationship between handgrip strength, urine c-peptide creatinine ratio, and blood glucose levels in adult females. Using ELISA, the study measured handgrip strength, blood glucose levels, and urine samples. Social demographic data was obtained through standard questionnaires, and statistical analysis was done using IBM 25 SPSS software with Pearson's correlation, linear regression at P=< 0.05, and T-test. The study found that handgrip strength (HGS) had a slight non-significant positive correlation with fasting blood sugar (FBS) (P=0.386). However, there was a significant correlation between HGS and 2 hours postprandial glucose (2HPG) in both dominant and non-dominant hands (P= 0.045 vs P= 0.017). Additionally, the study found that handgrip strength in the dominant hand was significantly stronger than that in the non-dominant hand (P= 0.001). On the other hand, the urinary C-peptide creatinine ratio (UCPCR) had no significant correlations with FBS and 2HPG. Handgrip strength measurements provide an indicative approach for glucose regulation and are a better biomarker for blood glucose regulation than UCPCR.Item Effect of Chronic Administration of Caffeine on Plasma Testosterone Level and Body Mass Index in Male Wistar R(Nigerian Society for Experimental Biology, 2021) Ayinde, T.O.; Balogun,M.E.; Afodun, A.M.; Ayinla, M.T.; Raji, A.O.; Liadi, M.T.; Belewu, M.A.Caffeine is one of the most frequently ingested pharmacological agents contained in several beverages and food items. With some positive biological effects on the body system, little knowledge is known on its effect on reproductive parameters. This present study therefore aimed to determine the effect of aqueous solution of caffeine on serum testosterone level and body mass index (BMI) in male Wistar rats. Twenty-one (21) male Wistar rats with average weight of 250 ± 20 g were obtained for this study and randomly grouped into three (3) of seven (7) rats each. Group A, served as the control and received 0.5 ml of normal saline while groups B and C served as test groups and received orally 0.38 mg/kg (low dose) and 1.14 mg/kg (high dose) of caffeine respectively, once daily for 14 days. There was significant increase (p<0.05) in body weight, BMI, and plasma testosterone levels of high dose (HD) and low dose (LD) compared to the control, while there was no significant (p>0.05) change in length of the animals compared to the control. Long time consumption of caffeine has potential to stimulate production of testosterone to possibly increase sex drive perhaps via hypothalamo-pituitary-testicular axis, and also increase BMI.Item Effects of Melatonin on Estrous Cycle Changes Induced by Ethanolic Extract of Cannabis-sativa in Female Wistar Rats.(Society for Experimental Biology of Nigeria, 2019) Oluwasola, A.,; Olayaki, L.A.; Ayinde, T.O.This study investigated the effects of melatonin and ethanolic extract of Cannabis-sativa (EECS) on oestrus cycle and reproductive hormones in reproduction of female rats. Twenty female rats were assigned into four groups of five animals each, such that the rats in groups I, II, III and IV received orally 1mL distilled water, 2mg/kg body weight (BW) of EECS, 2mg/kg BW of EECS plus 4mg/kg BW of melatonin and 4mg/kg BW of melatonin, respectively. Vaginal smear was taken daily to determine the Stages of oestrus cycle using light microscope (x400) between 8am-9am. Gonadotropin releasing hormone (GnRH), Luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E), progesterone and prolactin were also determined using standard methods. EECS significantly (p<0.05) lengthened the metestrus and diestrus phases of oestrus cycle. However, it significantly (p<0.05) shortened the proestrus and estrus phases. CS significantly (p<0.05) decreased GnRH, FSH, LH, E, progesterone and prolactin levels respectively. All these effects were ameliorated when combined with melatonin to the level comparable to the control. This study showed the gonadotoxic effects of EECS which could be mediated by endocrine disruption. However, these effects could be ameliorated by melatonin. Since the consumption of CS is increasing globally because of its medical uses leading to its legalization, therefore, consumption of melatonin as supplement is recommended for its users to prevent its gonadotoxic effects.Item Melatonin Mitigates Oxidative stress in Ethanolic Extract of Cannabis-Treated Female Wistar Rats(Nigerian Society of Biochemistry and Molecular Biology, 2019) Oluwasola, A.; Ayinde, T.O.; Olayaki, L.A.Cannabis sativa (CS) is one of the commonly abused substances by women of childbearing age. About 3.2% females are cannabis smokers in Nigeria, with varied consequences of congenital abnormalities due to interplay between oxidants, oxidants and antioxidants. This study investigated the effects of melatonin and cannabis on oxidative stress in female rats. Twenty female rats were assigned into four groups of five animals each. Groups I, II, III and IV received orally 1ml distilled water, 2mg/kg body weight (bw) of ethanolic extract of C. sativa (EECS), 2mg/kg bw of EECS plus 4mg/kg bw of melatonin and 4mg/kg bw of melatonin, respectively. Serum level of Superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione reductase, total antioxidant capacity (TAC), lactate dehydrogenase and Malondialdehyde (MDA) were assayed using standard methods. CS significantly (p<0.05) decreased catalase, SOD, glutathione peroxidase, glutathione reductase and TAC levels. But it significantly (p<0.05) increased lactate dehydrogenase and MDA levels. All these effects were ameliorated to the level comparable to the control when the extract was administered in combination with melatonin. CS showed alterations of biochemical parameters which was probably mediated by oxidative stress. However, these effects were ameliorated by melatonin. Since the consumption of CS is increasing globally because of its medical uses, thus, consumption of melatonin as supplement may be recommended.Item Report on a Double Phrygian Cap and Phrygian Prominence in a Nigerian Population(European Journal of Anatomy., 2018) Adam, M.A.; Olayemi, J.O.,; Airat, A.B.,; Kofoworola, K.Q.,; Ayinde, T.O.; Mustapha, A.M.,; Abdurrasheed, O.M.,It is debatable among anatomists whether the Phrygian cap of the gall bladder is an anomaly or anatomic variation with an incidence of 4.0% re ported. A 2-fold increase of 7.9% during a 5-year population study was observed in Lagos, Nigeria. A 48-year old male with minimal (non-acute) epi gastric discomfort was referred for ultrasound after suspicious cholecystitis. On radiological evalua tion, a double “Phrygian cap” was found by coinci dence. There is no recent emphasis on double Phrygian cap study, thus it is reported for its rarity and difficulty in arriving at conclusive diagnosis. Common imaging choice is mostly by multi-slice CT for biliary aspects and appendages. Most Phrygian caps are asymptomatic with little or no pathologic consequence. Surgical intervention (cholecystectomy) for a Phrygian cap is only indi cated in case of recurrent symptoms. To our knowledge, sparse anatomic and ultrasound litera ture exists on the Phrygian cap, thus the need for this rare case report.