A new model for alloxan-induced diabetes mellitus in rats.
No Thumbnail Available
Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Bangladesh Society of Physiologists
Abstract
Background:Alloxan is widely used to induce experimental
diabetes mellitus (DM) in animals with different grades of disease
severity by varying the dose of Alloxan used. This method has
however be questioned by recent research work as an appropriate
technique for the induction of diabetes. Objective: To provide a
simple, yet concise and reproducible experimental procedure
and model for Alloxan-induced DM in rats. Methods: The study
was divided into 2 separate experiments. Experiment 1: Alloxan
was administered, into four subgroups each (group 1- 100 mg of
Alloxan /kg of rat body weight, group 2- 120 mg/kg, group 3- 150
mg/kg, and group 4- 170 mg/kg); in each subgroup, the dose of
Alloxan was administered at different concentrations (20 mg/ml,
10 mg/ml, 5 mg/ml and 4 mg/ml) in groups of 10 rats each. The
pre-induction fasting period was also varied between groups.
Experiment 2:Following a pre-induction fasting period of 36 hours,
animals received 150 mg Alloxan /kg body weight and at a
concentration of 20 mg Alloxan/ ml. Result:Alloxan administered
intraperitoneally at 150 mg/kg of rat body weight, at 20 mg/ml
and following a pre-induction fast period of 36 hours yielded
the most favorably conditions with the least recorded mortality.
Conclusion: From the results of this study, it can be concluded
that alloxan is a diabetogenic drug with a strict protocol of use
in inducing a predictable DM in rats and as such, this model is a
standard and reproducible technique for the induction of DM in
experimental rats.
Description
Keywords
Citation
Alloxan, blood glucose, diabetes mellitus, pre-induction fast