Effects of MOF6 Fraction from Ethanolic Extract of the Leaves of Moringa oleifera against Sodium Arsenite-Induced Hepatotoxicity in Rats
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Date
2020
Authors
Ameen, Mubarak
Adelaja, Akinlolu
Mukadam, Abdulhamid
Muheen, Biliaminu
Ajiboye, Olaolu
Yahya, Rahmat
Abdulrahman, Bashir
Oyetunji, Oyepeju
Rotimi, Mojishola
Nejo, Victoria
Journal Title
Journal ISSN
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Publisher
Ethiopian Pharmaceutical Association
Abstract
Moringa oleifera (MO) is a plant of significant medicinal importance. The dried leaves of MO were
pulverized, extracted with ethanol and fractionated using column chromatography to provide seven
fractions (MOF1-7) with MOF6 having the best preliminary antioxidant potential. Therefore, this study
evaluated the hepatoprotective potentials of MOF6 in sodium arsenite (SA)-induced hepatotoxicity in rats.
Thirty-five adult male Wistar rats were randomly divided into seven groups of five rats each. Control
Group I received normal saline. Groups II and III received 20 mg/kg body weight (bw) of SA for 3 and 6
weeks, respectively. Groups IV and V received 20 mg/kg bw of SA for 3 weeks followed by treatment with
5.0 and 7.5 mg/kg bw of fraction MOF6, respectively, for 6 weeks. Groups VI and VII received only 5.0 and
7.5 mg/kg bw of fraction MOF6, respectively, for 6 weeks. Antioxidant (lipid peroxidation) and biochemical
analyses of liver enzymes of all rats were carried out after the completion of experimental procedures.
Results showed statistically significant lower mean values (p ≤ 0.05) of malondialdehyde (MDA), acid
phosphatase (ACP) and γ-glutamyl transferase (GGT) in rats of Groups IV and V compared with Group
III. However, there were statistically significant higher mean values (p ≤ 0.05) of alkaline phosphatase
(ALP) in Groups IV and V compared with Groups I and III. In conclusion, these results implied that
fraction MOF6 has antioxidant and hepatoprotective potentials. However, results of ALP analyses implied
that MOF6 possibly augmented SA-induced hepatotoxicity in rats.
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Keywords
Moringa oleifera, column fractions, sodium arsenite, hepatotoxicity, lipid peroxidation