Antibaterial activity of the GC-MS characterized essential oil from the leaves of Crassocephalum crepidiodes from North Central Nigeria
No Thumbnail Available
Date
2025-02-21
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Essential Oil & Plant Composition
Abstract
This study was carried out to isolate and evaluate the antibacterial activity of the leaf
essential oil of Crassocephalum crepidiodes against selected bacteria. Hydrodistillation of
500 g of sample of the plant’s leaves (for four hours) gave a yield of 0.10 %(w/w) of volatile
oil. The oil was characterized using GC-MS and the result showed abundant of
monoterpenoids in the oil and D-limonene (77.1 %) was the predominant constituent.
Meanwhile, linalool (2.6 %), α-pinene (5.5 %), β-caryophyllene (2.4 %), and α-terpineol
(2.6 %) were minor relative to the amount of limonene but are significant constituents of
the oil. The agar well diffusion method was used to determine the antibacterial activity
of the oil against Enterococcus faecalis, Bacillus cereus, Staphylococcus aureus, Streptococcus
pneumoniae, Salmonella typhi and Salmonella sp. Gram-positive bacteria were more
susceptible to the oil than Gram-negative bacteria. S. aureus was the most susceptible to
inhibition at the lowest concentration (25 %) used in this study. Therefore, the oil has
applications for the treatment of infections caused by the Gram-positive bacteria,
especially S. aureus.
Description
Keywords
Citation
Crassocephalum, crepidiodes, Staphylococcus, aureus, D- limonene