Browsing by Author "Sulaimon, F. A."

Now showing 1 - 12 of 12
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    Altered testicular histomorphometric and antioxidant levels following in vivo bisphenol-a administration
    (Arak University of Medical Sciences, 2021) Kadir, R. E.; Ojulari, S. L.; Gegele, A. T.; Lawal, A. I.; Sulu-Gambari, L.; Sulaimon, F. A.; Omotoso, G. O.
    Background: Bisphenol-A (BPA) is a pervasive environmental toxin that is used in the production processes of many consumables and equipment that are in daily application. The aim of this study was to determine the effects of BPA on the structural and functional integrity of the reproductive system in male Wistar rats and its interaction with melatonin. Methods: Adult female rats in pro-estrus phases were mated with adult male rats and the conception determined. The male pups were divided into two groups of A and B. These groups were further subdivided into six subgroups each. They were administered varying low doses of BPA (25 or 50mg/kg) and melatonin (10mg/kg) at neonatal and adolescent ages. The testes, epididymis and blood samples were collected for histological, semen and biochemical investigations, respectively. Results: The results show that BPA caused histological alterations, reduced quality and quantity of sperm cells, and induced oxidative stress at birth and adolescence. Conclusion:Bisphenol A exposure, even at low dose, is toxic to the male reproductive system, and melatonin administration did not significantly improve the alterations caused by the BPA.
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    Dichlorvos induced oxidative and neuronal responses in rats: mitigative efficacy of Nigella sativa (black cumin)
    (Physiological Society of Nigeria, 2018) Adana, M. Y.; Imam, A.; Ogunniyi, A.; Ibrahim, A.; Abdulmajeed, W. I.; Oyewole, L. A.; Lawan, A. H.; Sulaimon, F. A.; Ajao, M. S.
    Poisoning from Organophosphates (OPs), especially Dichlorvos (DDVP) has become endemic due to the increasing use in house hold and agricultural pests control, with most marked effects in the nervous system. However, it is evidenced that natural antioxidants are efficacious against OPs toxicity. Thus, this study investigated the possible antidotal efficacy of Nigella sativa oil (NSO) in Dichlovos (DDVP) induced oxidative and neuronal damages in Wistar rats. DDVP was administered at sub-chronic daily dosage of 8.8 mg/kg.bw for 7 days and a post-administration of NSO at 1 ml/kg.bw for the subsequent 7 days. The rats were euthanized on the 15thday, blood sample collected via cardiac puncture, centrifuged and the plasma used for biochemical analysis of total antioxidant capacity (TAC), reduced glutathione (GSH) and total reactive oxygen species (ROS), while the frontal, occipital and cerebellar cortices and the medulla were removed for histomorphological examinations. The results showed significant (P≤0.05) decrease in plasma TAC and GSH, while a significant (P≤0.05) increase in ROS was recorded, and some vacuolation around the neurons especially in the frontal and cerebellar cortices following DDVP exposure. However, post treatment with NSO was observed to be efficacious in the recovery of the oxidative activities and the neuro-architectural integrities. Thus, it can be concluded that the antioxidant capacity of NSO could be efficacious against OPs induced oxidative damages, especially in dichlorvos accidents.
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    Exogenous melatonin restored the cytoarchitectural integrity and biochemical activities of the cerebrum in sodium fluoride-induced toxicity.
    (Spanish Society of Anatomy and the Mexican Society of Anatomy, 2021) Ibrahim-Abdulkareem, R. A.; Okesina, A. A.; Sulaimon, F. A.; Imam, A.; Yawson, E.; Oluyomi, O.O.; Ajao M.S
    The cerebrum is responsible for motor, sensory and autonomic activities of the human body, and it is believed that fluoride exposure to the biological system can impede these functions. Therefore, it is imperative to introduce melatonin to limit the extent of fluoride toxicity on the cerebrum and understand the mechanism involved in the aforementioned process. Thirty-two rats were randomly selected into 4 groups (n=8, per group). Groups I-IV received oral administration of 0.2ml of normal saline (NS), 500ppm of sodium fluoride (NaF), concurrent administration of sodium fluoride and melatonin (NaF+MLT), and sodium fluoride before melatonin (NaF-MLT) for fourteen days respectively. At the end of these treatments, the rats were euthanized and cerebral tissues were excised for histological, histochemical and biochemical analyses. Sodium fluoride distorted the shapes and size of the cells and caused constriction of the blood vessels, as well as presence of vacuolations in the cells of the pyramidal layer of the cerebral cortex. However, melatonin was able to restore the cytoarchitecture of cells of the pyramidal layer of the cerebral cortex when administered concurrently and after the administration of sodium fluoride (NaF) respectively. Also, melatonin regulated the activities of Superoxide dismutase, Malondialdehyde and Glutathione peroxidase in the cerebrum. Sodium fluoride causes neurodegeneration in the cerebral cortex, and exogenous melatonin can ameliorate the injury caused by sodium fluoride on the cerebral cortex.
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    Exposure to varied cage-size habitats alters pain sensitivity and inflammation-related biomarkers.
    (Elsevier, 2020) Oyewole, A. L.; Oyafemi, K. O.; Badmus, K. S. J. O.; Omoleye, K. S.; Abubakar, M. F.; Adeniyi-Raheem, O.; Amedu, A.; Lawal, D. L.; Ijiyode, A. O.; Yussuf, A. O.; Ishola, S. S.; Sulaimon, F. A.; Alli-Oluwafuyi, A.O.; Nafiu, A.B.; Akinola, O.; Olajide, O.J.; Amin, A.; Abdulmajeed, W.I.; Michael, O.S.; Adeyanju, O.A.; Ogunjimi, G.L.
    Background: Nature and size of rodent cages vary from one laboratory or country to another. Little is however known about the physiological implications of exposure to diverse cage sizes in animal-based experiments. Method: Here, two groups of male Swiss mice (Control group – Cage stationed, and Test group – Cage migrated) were used for this study. The cage-migrated mice were exposed daily to various cage sizes used across labora tories in Nigeria while the cage-stationed mice exposed daily to different but the same cage size and shape. At the end of the 30 days exposure, top-rated paradigms were used to profile changes in physiological behaviours, and this was followed by evaluation of histological and biochemical metrics. Results: The study showed a significant (p < 0.05) decrease in blood glucose levels (at 60 and 120 min of oral glucose tolerance test) in the cage-migrated mice compared to cage-stationed mice. Strikingly, peripheral oxi dative stress (plasma malondialdehyde) and pain sensitivity (formalin test, hot-and-cold plate test, and von Frey test) decreased significantly in cage-migrated mice compared to cage-stationed animals. Also, the pro-in flammation mediators (IL-6 and NF-κB) increased significantly in cage-migrated mice compared to cage-sta tioned mice. However, emotion-linked behaviours, neurotransmitters (serotonin, noradrenaline and GABA), brain and plasma electrolytes were not significantly difference in cage-migrated animals compared to cage stationed mice. Conclusion: Taken together, these results suggest that varied size cage-to-cage exposure of experimental mice could affect targeted behavioural and biomolecular parameters of pain and inflammation, thus diminishing research reproducibility, precipitating false negative/positive results and leading to poor translational outcomes.
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    Histopathological and biochemical evaluation of the antidotal efficacy of Nigella sativa oil on organophosphate-induced hepatotoxicity
    (College of Health Sciences, Osun State University, 2017) Adana, M. Y.; Ajao, M. S.; Abdussalam, W. A.; Imam, A.; Amin, A. B.; Ibrahim, A.; Sulaimon, F. A.; Atata, J. A.
    Objective: The study was designed to investigate the effects of continuous exposure of dichlorvos (DDVP) on hepatic function and hepatic histomorphology, with the possible antidotal efficacy of Nigella sativa oil (NSO). Methods: Twenty four Wistar rats were randomly divided into four groups, with each group comprising of six rats. The groups were labelled as Sunflower oil (SFO), DDVP, DDVP+NSO and NSO. After 14 days of treatments, blood samples were collected, centrifuged and levels of ALP (Alkaline phosphatase), ALT (Alanine aminotransferase), AST (Aspartate aminotransferase) and GGT (γ-glutamyl-transferase) concentrations were estimated in the serum. The livers were removed and prepared for histopathological examinations and evaluation. Results: The findings of the study shows significant increase in the serum concentration of ALT, ALP, AST and GGT with a marked distortion in the hepatic architecture in rats administered with DDVP. However, Nigella sativa oil (NSO) was observed to ameliorate the levels of impairment in the assessed hepatic function parameters and relatively restoration in the hepatic architecture in DDVP+NSO treated animals when compared to the control and group administered with DDVP only. Conclusion: The study concludes that impaired liver functions and histomorphological tissue distortions observed in the experimental rats following DDVP exposure were ameliorated following the administration of NSO.
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    Honey and levodopa comparably preserved substantia nigra pars compacta neurons through the modulation of nuclear factor erythroid 2-related factor 2 signaling pathway in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease model
    (Korean Association of Anatomists, 2024) Sulaimon, F. A.; Ibiyeye, R. Y.; Imam, A.; Oyewole, A. L.; Imam, A. L.; Shehu, M.; Biliaminu, S. A.; Kadir, R. E.; Omotoso, G. O.; Ajao, M. S.
    : Parkinson’s disease (PD) affects about 8.5 million individuals worldwide. Oxidative and inflammatory cascades are implicated in the neurological sequels, that are mostly unresolved in PD treatments. However, proper nutrition offers one of the most effective and least costly ways to decrease the burden of many diseases and their associated risk factors. Moreover, prevention may be the best response to the progressive nature of PD, thus, the therapeutic novelty of honey and levodopa may be prospective. This study aimed to investigate the neuroprotective role of honey and levodopa against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced oxidative stress. Fifty-four adult male Swiss mice were divided into control and PD model groups of 27 mice. Each third of the control mice either received phosphate buffered saline, honey, or levodopa for 21 days. However, each third of the PD models was either pretreated with honey and levodopa or not pretreated. Behavioral studies and euthanasia were conducted 2 and 8 days after MPTP administration respectively. The result showed that there were significantly (P<0.05) higher motor activities in the PD models pretreated with the honey as well as levodopa. furthermore, the pretreatments protected the midbrain against the chromatolysis and astrogliosis induced by MPTP. The expression of antioxidant markers (glutathione [GSH] and nuclear factor erythroid 2-related factor 2 [Nrf2]) was also significantly upregulated in the pretreated PD models. It is thus concluded that honey and levodopa comparably protected the substantia nigra pars compacta neurons against oxidative stress by modulating the Nrf2 signaling molecule thereby increasing GSH level to prevent MPTP-induced oxidative stress.
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    Muscle Tissues: In Histology Practical Manual
    (Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin., 2024-04) Lewu, F. S.; Ibiyeye, R. Y.; Danwahab, O. A.; Imam, A.; Jaji-Sulaimon, R.; Sulaimon, F. A.; Ajao, M. S.; Omotoso. G. O.
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    Nigella sativa oil ingestion mitigates aluminum chloride (alcl3) induced cerebellar oxidative, neurogenic damages and impaired motor functions in Wistar rats
    (Association of Anatomical Societies of Africa, 2022) Imam, A.; Sulaimon, F. A.; Shehu, M.; Busari, M.; Oyegbola, C.; Okesina, A. A.; Afodun A. M.; Adana, M. Y.; Ajao, M. S.
    Varying neurological effects, and impairments to motor functions, neurochemistry and neuromorphology have been associated with Aluminium chloride (AlCl3) induced neurotoxicity. This study aims to investigate the efficacy of Nigella sativa oil (NSO) in AlCl3 induced cerebellar toxicity in rats. Thirty-two male Wistar rats were randomly divided into four groups and received: normal saline; 100 mg/kg.bw of AlCl3; 100 mg/kg.bw AlCl3 and 1 ml/kg.bw of NSO; and 1 ml/kg.bw, orally and daily for fourteen days. On the 13th day of the experiment, the rats were each exposed to a single trial of the Open Field Test (OFT), of which line crossing frequency, rearing frequency, and freezing period were recorded as measures of exploratory and locomotive behaviours of the animals. By day 15, the rats were euthanized, their brains were excised, the cerebellum dissected from five brains of each group, and homogenized for biochemical evaluations of nitric oxide (NO) metabolites and total reactive oxygen species (ROS) levels. The remaining three brains in each group were processed for histology and Ki67 immunohistochemistry investigations. The results of this study shows that AlCl3 impaired motor related behaviours in the AlCl3 exposed animals, by significantly reducing the line crossing and rearing frequencies, and increasing the freezing period. This effect was observed to be mitigated in the animal group that received NSO following AlCl3 administration, as the animals showed improved motor behaviours. AlCl3 also caused an increase in the cerebellar activities of NO and ROS, while it depleted Ki67 expressions and caused neurodegenerative-like effects in the cerebellar histoarchitecture of the exposed animals. Intervention with NSO depleted ROS/NO levels and protected the cerebellum from the nitrosative and oxidative stress induced by AlCl3. NSO was also observed to preserve the cerebellar cortex histoarchitecture and neurogenic morphology against the neurodegenerative effect of AlCl3. It can be concluded that NSO, with its high efficacy against oxidative stress and neuroinflammation, is a potent natural therapeutic agent in aluminum and heavy metal neurotoxicity.
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    Phoenix dactylifera polyphenols ameliorates monosodium glutamate induced cell damage in the dentate gyrus
    (Krishna Institute of Medical Sciences, 2021) Usman, R. Y.; Sulaimon, F. A.; Okesina, A. A.; Imam, A.; Imam, A. L.; Ajao M. S
    Background: Monosodium Glutamate (MSG) use is quite alarming considering the cascades of toxicity that arises from it. However, this study demonstrated the possible ameliorative activities of polyphenols of Phoenix dactylifera (PPD) on MSG-induced dentate gyrus degeneration in male adult Wistar rats. Aim and Objectives: To check the effects of PPD on MSG induced dentate gyrus neuronal damage in adult male Wistar rats. Material and Methods: Groups A to D of adult male rats underwent 14-day treatment of Normal Saline (NS), 500 mg ̸ kg PPD, 4 mg ̸g of MSG only, and 4 mg̸g MSG and 500 mg/kg PPD (MSG+PPD) concurrently. Group E received 500 mg ̸ kg PPD for a 14-day period prior to another 14-day of 4mg ̸ gMSG (PPD then MSG). Results: PPD was able to ameliorate the toxic effect of MSG as evidence of better cellular integrity, minimal cell vacuolations, minimized dispersed Nissl bodies and deeply stained Nissl bodies were observed upon PPD administration. It was also observed that it reduced the proliferation of reactive oxygen species, proteins and DNAdamage in the cells of the dentate gyrus. Conclusion: The study concluded that PPD was able to ameliorate the degeneration induced by MSG in the dentate gyrus of Wistar rats
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    Subchronic dichlorvos-induced Cardiotoxicity in Wistar rats: Mitigative efficacy of Nigella sativa oil.
    (2018) Adana, M. Y.; Imam, A.; Busari, M. O.; Ajibola, M. I.; Ibrahim, A.; Sulaimon, F. A.; Ajao, M. S.
    BACKGROUND: Accidental poisoning from indiscriminate use of organophosphates have become endemic in recent decades, most especially in developing nations, coupled with the limitations of the availability of satisfactory antidotes. AIM OF THE STUDY: Thus, we investigated the cardioprotective efficacy of Nigella sativa oil (NSO) following dichlorvos dichlorvos (DDVP)‑induced cardiotoxicity in Wistar rats. MATERIALS AND METHODS: A total of 24 Wistar rats were randomly divided into four groups (n = 6); the control was administered sunflower oil (1 ml/kg), DDVP (8.8 mg/kg) to the experimental Group I, whereas DDVP + NSO (8.8 mg/kg +1 ml/kg) and NSO (1 ml/kg) was administered orally to the experimental Groups II and III, respectively. The animals were euthanized; blood was transcardially collected from the right atrium, centrifuged, and plasma extracted to analyze levels of total cholesterol (TC), triglycerides, high‑density lipoprotein cholesterol, and low‑density lipoprotein cholesterol (LDL‑C). While cardiac muscle tissue was collected from the left heart, processed and stained for general architecture (hematoxylin and eosin) and elastic morphology (orcein). RESULTS: DDVP significantly (P ≤ 0.05) increased the plasma levels of TC, LDL, atherogenic and atherosclerotic indices (TC/HDL‑C and LDL‑C/HDL‑C ratios), but this was prevented by co-administration with NSO. Histological investigations showed that DDVP resulted in the pathological appearance of cardiac tissues, such as the lack of striations, myocardial hemorrhage, and necrosis‑like features. CONCLUSION: It can be concluded that NSO was able to attenuate DDVP‑induced cardiotoxicity.
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    Thymoquinone ameliorate oxidative stress, GABAergic neuronal depletion and memory impairment through Nrf2/ARE signaling pathway in the dentate gyrus following cypermethrin administration
    (BMC Neuroscience, 2024) Imam, A. L.; Okesina, A. A.; Sulaimon, F. A.; Imam, A.; Ibiyeye, R. Y.; Oyewole, L. A.; Biliaminu, S. A.; Shehu, M.; Alli, A. O.; Omoola, O. O.; Ajao, M.S.
    Background Exposure to chemical toxins, including insecticides, harms bodily organs like the brain. This study examined the neuroprotective of thymoquinone on the cypermethrin’s harmful effects on the histoarchitecture of the dentate gyrus and motor deficit in the dentate gyrus. Methods Forty adult male rats (180–200 g) were randomly divided into 5 groups (n=8 per group). Groups I, II, III, IV, and V received oral administration of 0.5 ml of phosphate-buffered saline, cypermethrin (20 mg/kg), thymoquinone (10 mg/kg), cypermethrin (20 mg/kg)+thymoquinone (5 mg/kg), and cypermethrin (20 mg/kg)+thymoquinone (10 mg/kg) for 14 days respectively. The novel object recognition test that assesses intermediate-term memory was done on days 14 and 21 of the experiment. At the end of these treatments, the animals were euthanized and taken for cytoarchitectural (hematoxylin and eosin; Cresyl violet) and immunohistochemical studies (Nuclear factor erythroid 2-related factor 2 (Nrf2), Parvalbumin, and B-cell lymphoma 2 (Bcl2). Result The study shows that thymoquinone at 5 and 10 mg/kg improved Novelty preference and discrimination index. Thymoquinone enhanced Nissl body integrity, increased GABBAergic interneuron expression, nuclear factor erythroid 2-derived factor 2, and enhanced Bcl-2 expression in the dentate gyrus. It also improved the concentration of nuclear factor erythroid 2-derived factor 2, increased the activities of superoxide dismutase and glutathione, and decreased the concentration of malondialdehyde level against cypermethrin-induced neurotoxicity. Conclusion thymoquinone could be a therapeutic agent against cypermethrin poisoning.
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    Thymoquinone Ingestions Reversed Inflammation Driven Glia activation and Impaired Cognitive associated behavior in Cypermethrin Exposed Rats.
    (Uskudar University, 2024) Imam, A. L.; Okesina, A. A.; Sulaimon, F. A.; Imam, A.; Ibiyeye, R. Y.; Adana, M. Y.; Omoola, O. O.; Ajao, M. S.
    Background: Pyrethroids pose health risks to humans. Therefore, it is imperative to assess the preventive benefits of thymoquinone against neurotoxicity induced by cypermethrin- in the hippocampal dentate gyrus. Methods: Forty male adult Wistar rats with an average weight of 180-200g were randomly allocated to five (5) groups, and each comprising eight rats (n=8 per group). The groups were designated as follows, through oral administrations for 14 days: 0.5ml phosphate- buffered saline (PBS) was given to group one; Group two received 20mg/kg of cypermethrin (CYM); Group three received 10 mg/kg of thymoquinone (THQ); Group four received 20 mg/kg of cypermethrin followed by 10mg/kg of thymoquinone (CYM-10mgTHQ); and Group five received 20mg/kg and 5mg/kg cypermethrin and thymoquinone respectively (CYM-5mgTHQ). Behavioral, histological, immunohistochemical, and biochemical analyses were conducted post-treatment. Results: Cypermethrin administration caused the rise in pro-inflammatory cytokine TNF-α and increased expression of astrocytes, microglia, and pro-apoptotic protein Bax. Additionally, cypermethrin reduced levels of anti-inflammatory cytokine IL-10, acetylcholinesterase (AChE) activity, and nuclear factor erythroid 2-related factor 2 (Nrf2). cytoarchitectural disruption of dentate gyrus and decreased Nrf2 expression were observed. Cognitive deficits were evident. Thymoquinone treatment attenuated TNF-α elevation, reduced astrocyte, microglial, and Bax expression, and increased IL-10, AChE, and Nrf2 levels. Conclusion: Thymoquinone demonstrated anti-inflammatory and anti-apoptotic effects against cypermethrin-induced neurotoxicity, improving cognitive function in rats.