Thymoquinone ameliorate oxidative stress, GABAergic neuronal depletion and memory impairment through Nrf2/ARE signaling pathway in the dentate gyrus following cypermethrin administration
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Date
2024
Journal Title
Journal ISSN
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Publisher
BMC Neuroscience
Abstract
Background Exposure to chemical toxins, including insecticides, harms bodily organs like the brain. This study
examined the neuroprotective of thymoquinone on the cypermethrin’s harmful effects on the histoarchitecture of the
dentate gyrus and motor deficit in the dentate gyrus.
Methods Forty adult male rats (180–200 g) were randomly divided into 5 groups (n=8 per group). Groups I, II, III, IV,
and V received oral administration of 0.5 ml of phosphate-buffered saline, cypermethrin (20 mg/kg), thymoquinone
(10 mg/kg), cypermethrin (20 mg/kg)+thymoquinone (5 mg/kg), and cypermethrin (20 mg/kg)+thymoquinone
(10 mg/kg) for 14 days respectively. The novel object recognition test that assesses intermediate-term memory was
done on days 14 and 21 of the experiment. At the end of these treatments, the animals were euthanized and taken for
cytoarchitectural (hematoxylin and eosin; Cresyl violet) and immunohistochemical studies (Nuclear factor erythroid
2-related factor 2 (Nrf2), Parvalbumin, and B-cell lymphoma 2 (Bcl2).
Result The study shows that thymoquinone at 5 and 10 mg/kg improved Novelty preference and discrimination
index. Thymoquinone enhanced Nissl body integrity, increased GABBAergic interneuron expression, nuclear factor
erythroid 2-derived factor 2, and enhanced Bcl-2 expression in the dentate gyrus. It also improved the concentration
of nuclear factor erythroid 2-derived factor 2, increased the activities of superoxide dismutase and glutathione, and
decreased the concentration of malondialdehyde level against cypermethrin-induced neurotoxicity.
Conclusion thymoquinone could be a therapeutic agent against cypermethrin poisoning.
Description
Keywords
Cypermethrin, Thymoquinone, Cresyl fast violet, GABBAergic interneuron, Dentate gyrus
Citation
Imam, A. L., Okesina, A. A., Sulaimon, F. A., Imam, A., Ibiyeye, R. Y., Oyewole, L. A., Biliaminu, S. A., Shehu, M., Alli, A. O., Omoola, O. O., & Ajao, M.S. (2024). Thymoquinone ameliorate oxidative stress, GABAergic neuronal depletion and memory impairment through Nrf2/ARE signaling pathway in the dentate gyrus following cypermethrin administration. BMC Neuroscience, 25:45