Browsing by Author "Ojulari, L.S."

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    A new model for alloxan-induced diabetes mellitus in rats.
    (Journal of Bangladesh Society of Physiologists, 2019) Ojulari, L.S.; Oladeru, O.O.,; Ayinde, T.O.; Alade I.O.; Kadir, R.E.; Dangana, O.E.
    Background:Alloxan is widely used to induce experimental diabetes mellitus (DM) in animals with different grades of disease severity by varying the dose of Alloxan used. This method has however be questioned by recent research work as an appropriate technique for the induction of diabetes. Objective: To provide a simple, yet concise and reproducible experimental procedure and model for Alloxan-induced DM in rats. Methods: The study was divided into 2 separate experiments. Experiment 1: Alloxan was administered, into four subgroups each (group 1- 100 mg of Alloxan /kg of rat body weight, group 2- 120 mg/kg, group 3- 150 mg/kg, and group 4- 170 mg/kg); in each subgroup, the dose of Alloxan was administered at different concentrations (20 mg/ml, 10 mg/ml, 5 mg/ml and 4 mg/ml) in groups of 10 rats each. The pre-induction fasting period was also varied between groups. Experiment 2:Following a pre-induction fasting period of 36 hours, animals received 150 mg Alloxan /kg body weight and at a concentration of 20 mg Alloxan/ ml. Result:Alloxan administered intraperitoneally at 150 mg/kg of rat body weight, at 20 mg/ml and following a pre-induction fast period of 36 hours yielded the most favorably conditions with the least recorded mortality. Conclusion: From the results of this study, it can be concluded that alloxan is a diabetogenic drug with a strict protocol of use in inducing a predictable DM in rats and as such, this model is a standard and reproducible technique for the induction of DM in experimental rats.
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    Androgenic Effects of Aqueous seed extract of Moringa oleifera in Male Wistar Rats
    (Published by School of Medicine and Health Sciences, University of Papua, New Guinea, 2022) Ayinla M.T; Muhammad, A.S.; Ayinde, T.O.; Ojulari, L.S.; Owoyele, B.V.; Asuku, A.O.; Adebisi, R.O.; Badmus, O.A.; Krishnamurthy, R.
    Androgenic effects of Aqueous Seed-extract of Moringa oleifera (ASMO) in male Wistar rats were investigated. Eighteen (18) male Wistar rats weighing 200-240g were used for this study. The rats were divided into three (3) groups: Control (Group 1) that received 10 ml/kg of normal saline, Group 2: received low dose of ASMO (200mg/kg), and Group 3: received high dose of ASMO (500mg/kg). The animals were treated for twenty-eight days. On the 29th day, the rats were sacrificed and the testes were carefully removed for semen and biochemical analysis. Body weight, reproductive and vital organ weights were determined. Sperm parameters (motility, morphology, count and viability), tissue testosterone, luteinizing hormone (LH), Malondialdehyde (MDA) and Catalase were also determined using standard methods. Data were analysed using one-way ANOVA followed by Duncan new multiple range post hoc test. The result showed ASMO significantly increased (p<0.05) the final body weight, weight of reproductive and vital organs. Moreover, 200mg/kg body weight dose of ASMO significantly increased (p<0.05) the sperm parameters but 500mg/kg body weight dose significantly decreased it. ASMO also caused a significant dose dependent increase (p<0.05) in testosterone and catalase level but a significant decrease (p<0.05) in MDA and LH level compared with the control. In conclusion, this study revealed that ASMO has androgenic effects in male rats and thus provides a basis for the traditional use of Moringa oleifera in the management of male sexual disorders.
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    COMPARATIVE ANALYSIS OF HANDGRIP STRENGTH AND URINE C-PEPTIDE CREATININE RATIO AS BIOMARKERS FOR GLUCOSE REGULATION IN YOUNG ADULT FEMALES IN THE UNIVERSITY OF ILORIN: A CROSS-SECTIONAL STUDY
    (Society for Experimental Biology of Nigeria, 2023) Ojulari, L.S.; Sulaiman, S.E.; Ayinde, T.O.; Kadir, R.E.; Hidaayah O. Jimoh-Abdulghaffaar, H.O.; Sulaiman, H.
    Handgrip strength (HGS) is a robust biomarker predicting future disability, metabolic syndrome, and diabetes. Urinary C-peptide creatinine ratio (UCPCR) emerges as a novel, non-invasive tool under exploration for assessing beta cell function and glucose regulation. Despite their significance in gauging muscle strength, mass, and overall metabolic function, gaps remain in understanding the full extent of handgrip strength and UCPCR's efficiency. This study aimed to identify a better biomarker for glucose regulation by studying the relationship between handgrip strength, urine c-peptide creatinine ratio, and blood glucose levels in adult females. Using ELISA, the study measured handgrip strength, blood glucose levels, and urine samples. Social demographic data was obtained through standard questionnaires, and statistical analysis was done using IBM 25 SPSS software with Pearson's correlation, linear regression at P=< 0.05, and T-test. The study found that handgrip strength (HGS) had a slight non-significant positive correlation with fasting blood sugar (FBS) (P=0.386). However, there was a significant correlation between HGS and 2 hours postprandial glucose (2HPG) in both dominant and non-dominant hands (P= 0.045 vs P= 0.017). Additionally, the study found that handgrip strength in the dominant hand was significantly stronger than that in the non-dominant hand (P= 0.001). On the other hand, the urinary C-peptide creatinine ratio (UCPCR) had no significant correlations with FBS and 2HPG. Handgrip strength measurements provide an indicative approach for glucose regulation and are a better biomarker for blood glucose regulation than UCPCR.
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    Moringa oleifera attenuates biochemical and histological changes associated with the pancreas in nicotine-treated rats.
    (2018) Omotoso, G.O.; Adunmo, G.O.; Ojulari, L.S.; Olawuyi, T.S.; Lewu, S.F.; Jaji-Sulaimon, R.; Sulaimon, F.A.; Gbadamosi, I.T.; Onoja, P.
    Objective: The study was undertaken in order to evaluate the beneficial potential of Moringa oleifera, in nicotine-induced pancreatic injury. Method: Forty-five adult female albino rats were divided into 5 groups A-E, each group having nine rats. Group Areceived normal saline; group B received 6.88 mg/kg of nicotine intraperitoneally (i.p); group C received 6.88 mg/kg of nicotine i.p. and 200 mg/kg of Moringa oleifera leaf powder dissolved in 2 ml of normal saline (orally); group D received 13.76 mg/kg of nicotine i.p., while group E received 13.76 mg/kg of nicotine i.p. and 200 mg/kg of Moringa oleifera leaf powder dissolved in 2 ml of normal saline (orally). Treatment was for 8 days and the rats were sacrificed after 24 hours of termination of study. Intracardial blood specimens were obtained to analyse blood glucose, while the pancreas was excised and either fixed in 4% paraformaldehyde for histology or sucrose solution and homogenised for biochemical analysis of lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) enzymes. Results: In comparison with the Control, animals treated with low dose of nicotine with or without Moringa oleifera and those treated with high dose of nicotine plus Moringa oleifera had reduction in body weights (p>0.05), while marked reduction in pancreatic weights was noted in low dose nicotine (p<0.05) and both nicotine groups co-treated with Moringa oleifera (p<0.05). There were no significant changes in the levels of blood glucose and pancreatic G-6-PDH levels, while significant reduction occurred in pancreatic LDH levels in nicotine-treated rats (p<0.05). However, LDH improved following co administration with Moringa oleifera. Observation of the histology of the pancreas revealed atrophy of intercalated ducts, poorly delineated and disintegrating islet of Langerhans in animals treated with the higher dose of nicotine, while changes in pancreatic tissue in animals co-treated with Moringa oleifera were not as severe as the nicotine-treated animals. Conclusion: Moringa oleifera leaf decoction minimally ameliorates morphological and biochemical changes associated with nicotine-induced pancreatic damage.
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    Serum C-reactive protein and cholesterol as predictors of severity in childhood Falciparum malaria infestation among Nigerians
    (Faculty Board of Pathology, National Postgraduate Medical College of Nigeria, 2012) • Biliaminu, S.A.; Shittu, A.O.; Olatunbosun, L.O.; Abdulazeez, I.M.; Sani, M.A.; Okesina, A.B.; Akande, A.A.; Omokanye, K.O.; Ojulari, L.S.
    Background: C-Reactive Protein (CRP) is a highly sensitive marker of acute inflammation and has been observed to rise significantly during malaria infections. It is valuable both diagnostically and epidemiologically. In contrast, red cell cholesterol, though not affecting membrane function, may impede malaria invasion. Traditional methods of grading malaria severity using parasite count have limitations, necessitating alternative indicators. Materials and Methods: The study involved 120 pediatric patients with acute malaria, categorized into mild, moderate, and severe groups (40 each). CRP and cholesterol levels were measured and correlated with malaria parasite count and severity. Results: Mean ages for the mild, moderate, and severe groups were 10.3, 7.1, and 3.8 years, respectively. CRP levels increased significantly, while cholesterol levels decreased with increasing malaria severity. CRP positively correlated with parasite count and scoring, while cholesterol showed a negative correlation. Conclusion: Serum CRP is a reliable indicator of malaria severity. Cholesterol also serves as a potential severity marker, although less prominently. These markers may aid diagnosis and therapeutic monitoring of falciparum malaria.
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    The effect of Momordical charantia leaf extract on white blood cell count in albino rats
    (Department of Applied and Environmental Biology, Rivers State University of Science and Technology, Port-Harcourt, Nigeria, 2010) Ojulari, L.S.; Okesina , K.B.; Bisiriyu, M.A.; Ayinla M.T
    Momordical charantia is among the most bitter fruits. Several studies revealed that this plant has anti-ulcer, anti-diabetic, antifungal, anti-leukemia, anti-protozoal, antibacterial, antifertility, antiviral and hypoglycemic effects. we aim to study the effect of MC on the immune system. The study involved 25 rats and they were divided into 5 groups each comprising of 5 rats. The aqueous extract of Momordical charantia was administered orally with syringes and cannula to 4 groups (A,B,C,D) at different doses (50mg/kg, 100mg/kg, 120mg/kg and 140mg/kg body weight per day respectively) to investigate its effect on white blood cells count and the last group served as the control and they were given drug vehicle (normal saline daily). The result showed a significant decrease (p<0.05) in mean white blood cells count of all experimental animals when compared to the control group at the end of administration which lasted for two weeks. The decrease in white blood cells count was most significant at the highest dose administered (140mg/kg body weight). There was a significant increase (p<0.05) in mean neutrophil count of two groups (A and C) when compared to the control group, while the remaining two groups showed no significant change (p>0.05). This study showed that M.charantia caused significant changes in white blood cells count when administered orally and that this effect was probably dose dependent.
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    The effect of Momordical charantia leaf extract on white blood cell count in albino rats.
    (Published by Department of Applied and Environmental Biology, Rivers State University of Science and Technology, Port-Harcourt, Nigeria., 2010) Ojulari, L.S.; Okesina, K.B.; Bisiriyu, M.A.; Ayinla M.T
    Momordical charantia is among the mist bitter of all fruits. Several studies revealed that, this plant has anti-ulcer, anti-diabetic, antifungal, anti-leukemic, anti-protozoal, antibacterial, anti-fertility, antiviral and hypoglycemic effects. We aim to study the effect of MC on the immune system. This study involved 25 rats and they were divided into 5 groups each comprising of 5 rats. The aqueous extract of Momordical charantia was administered orally with syringes and cannula to 4 groups (A,B,C,D) at different doses (80mg/kg, 100mg/kg, 120mg/kg and 140mg/kg body weight per day respectively) to investigate its effect on white blood cells count and the last group served as the control and they were given drug vehicle (normal saline) only. The results showed a significant decrease (p<0.05) in mean white blood cells count of all experimental animals when compared to the control group at the end of the administration which lasted for two weeks. The decrease in white blood cells count was most significant at the highest dose administered (140mg/kg body weight). There was a significant increase (p<0.05) in mean neutrophils count of two groups (A and C) when compared to the control group while the remaining two groups showed no significant change (p>0.05). the study showed that M.charantia caused significant changes in white blood cells count when administered orally and that this effect was probably dose dependent.