Browsing by Author "Lawal I.A."

Now showing 1 - 5 of 5
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    Altered testicular histomorphometric and antioxidant levels following in vivo Bisphenol-A administration
    (School of Medicine, Arak University of Medical Sciences, 2021) Kadir R.E.; Ojulari L.S.; Gegele T.A.; Lawal I.A.; Sulu-Gambari L.; Sulaimon F.A.; Omotoso G.O.
    Background: Bisphenol-A (BPA) is a pervasive environmental toxin that is used in the production processes of many consumables and equipment that are in daily application. The aim of this study was to determine the effects of BPA on the structural and functional integrity of the reproductive system in male Wistar rats and its interaction with melatonin. Methods: Adult female rats in pro-estrus phases were mated with adult male rats and the conception determined. The male pups were divided into two groups of A and B. These groups were further subdivided into six subgroups each. They were administered varying low doses of BPA (25 or 50mg/kg) and melatonin (10mg/kg) at neonatal and adolescent ages. The testes, epididymis and blood samples were collected for histological, semen and biochemical investigations, respectively. Results: The results show that BPA caused histological alterations, reduced quality and quantity of sperm cells, and induced oxidative stress at birth and adolescence. Conclusion: Bisphenol A exposure, even at low dose, is toxic to the male reproductive system, and melatonin administration did not significantly improve the alterations caused by the BPA
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    Comparative oestrogenic effects of Allium sativum and Allium cepa in ovariectomised rats
    (Association of Anatomical Societies of Africa, 2022) Kadir R.E.; Ojulari L.S.; Lawal I.A.; Sulu-Gambari L.; Hussein A/; Hamza A.; Aliagan I.A.; Oyewole L.A.
    Oestrogens are steroid hormones that influence the growth, differentiation, and functioning of many target organs, including the male and female reproductive organs. Menopause, an important sign of aging in women is characterized by oestrogen depletion, which is associated with many menopausal problems. Oestrogen supplements that are mostly used are not readily safe and have been reported to cause serious health hazards Phytoestrogens are plant compounds that are essentially correspondent to oestradiol (17-β oestradiol) and can relate with oestrogen receptors to elevate and/or prohibit oestrogenic responses. This study therefore aimed at evaluating the possible therapeutic efficacy of Allium sativum and Allium cepa on oestrogenic activities of adult ovariectomised wistar rats. Adult Wistar rats were ovariectomized, then administered various doses of the extracts for 28days, then sacrificed and tissues harvested for analysis. Results shows Allium cepa and quercetin to increase endometrial thickness, increase neural cells in synaptophysin stained cells of hippocampus and dentate gyrus. A conclusion of Allium cepa possessing similar estrogenic properties as estradiol group in these ovariectomized rats was drawn, which can offer some ameliorative effects of estrogen deficiency.
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    Comparative oestrogenic effects of Allium sativum and Allium cepa in ovariectomised rats
    (Association of Anatomical Societies of Africa, 2022) Kadir R.E.; Ojulari L.S.; Lawal I.A.; Sulu-Gambari L.; Hussein A.; Hamza A.; Aliagan I.A.; Oyewole L.A.
    Oestrogens are steroid hormones that influence the growth, differentiation, and functioning of many target organs, including the male and female reproductive organs. Menopause, an important sign of aging in women is characterized by oestrogen depletion, which is associated with many menopausal problems. Oestrogen supplements that are mostly used are not readily safe and have been reported to cause serious health hazards Phytoestrogens are plant compounds that are essentially correspondent to oestradiol (17-β oestradiol) and can relate with oestrogen receptors to elevate and/or prohibit oestrogenic responses. This study therefore aimed at evaluating the possible therapeutic efficacy of Allium sativum and Allium cepa on oestrogenic activities of adult ovariectomised wistar rats. Adult Wistar rats were ovariectomized, then administered various doses of the extracts for 28days, then sacrificed and tissues harvested for analysis. Results shows Allium cepa and quercetin to increase endometrial thickness, increase neural cells in synaptophysin stained cells of hippocampus and dentate gyrus. A conclusion of Allium cepa possessing similar estrogenic properties as estradiol group in these ovariectomized rats was drawn, which can offer some ameliorative effects of estrogen deficiency.
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    Cytoarchitectural differences in reproductive organs of some polycystic ovary-like induced animal models
    (Elsevier Limited, 2024) Kadir E.R.; Yakub A.D.; Ojulari L.S.; Hussein A.O.; Lawal I.A.; Jaji-Sulaimon R.; Ajao M.S.
    Polycystic ovary syndrome (PCOS) is the most common gynaecological, endocrine disorder that occurs during reproductive age and is a significant cause of anovulatory infertility. Letrozole is an aromatase inhibitor which negates the action of the aromatase enzyme, which results in the buildup of male hormones (testosterone) in the females, causing hyperandrogenism, which is a hallmark of Polycystic Ovarian Syndrome. Mifepristone (RU486) is a progestin antagonist that acts to arrest the actions of the progesterone hormone, resulting in follicular atresia and anovulation. DHEA is an androgen which was also administered in a bid to cause hyperandrogenism in the rats.This study aimed to evaluate the effects of these hormones on the cytoarchitecture of the ovaries and uterus to assess their various PCOS-like histological features.Animals were grouped mainly into three: Letrozole, Mifepristone and DHEA groups, which were further divided into two subgroups each, administered low and high doses of letrozole orally, Mifepristone and Dehydroepiandosterone (DHEA) subcutaneously. Each of the subgroups also had a comparison control group. Following the completion of administration, the Wistar rats were euthanized, and their ovaries and uterus were collected for histological analysis.Increased proliferation of ovarian follicles was noted in the treated groups compared to control, as well as thickening of the endometrial layer.
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    Restorative effects of melatonin on bisphenol A-induced interference of gene expression in hypothalamic pituitary axis following early exposure
    (International Journal of Health Sciences, 2024) Kadir E.R.; Yakub A.D.; Imam A.; Olajide O.J.; Lawal I.A.; Oyewole A.L.; Hussein M.O.; Ojulari L.S.; Ajao M.S.
    Background: Bisphenol-A is a standard monomer used industrially in manufacturing plastics and epoxy resins, and it is widely used in food preservation and packaging. There is a global increase in its use due to increased demand and the growth in world population. Bisphenol A is an endocrine-disrupting chemical miming the endogenous estradiol hormone. However, due to its exposure to the environment, food and other consumables, its effects on reproductive health have been a growing area of interest. Melatonin regulates sleep-wake cycles and plays essential physiological roles in the body through its antioxidative, anti-cancer and neuroprotective properties. This research aims to ascertain the impact of Bisphenol A on the hypothalamic-pituitary-ovarian axis and determine melatonin's function on possible BPA-induced effects. Methods: Six adult male Wistar rats and 12 adult female Wistar rats of proven fertility were bred and organized into groups. Litters were divided into seven groups, each comprising six rats. These animals were subjected to subcutaneous injections of high and low doses of bisphenol A from postnatal days 0-3, then oral melatonin. The rats were allowed to mature into full-grown adults and euthanized at 120 ±4 days. The serum and hypothalamic-pituitary-ovarian tissues were collected for various assays, histology and genetic studies. Results: Compared to the control groups, groups administered varying doses of bisphenol A showed significant overexpression of estrogen and androgen receptors. Administration of Melatonin showed some reversal and reparative effects on BPA-induced damage of the hypothalamic pituitary ovarian axis. Conclusion: Elevated estrogen receptor levels induced by Bisphenol A altered receptor function, ultimately impairing hormonal cascades that regulate reproductive functions. Melatonin showed some promising reparative effects.