Restorative effects of melatonin on bisphenol A-induced interference of gene expression in hypothalamic pituitary axis following early exposure

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Date

2024

Journal Title

Journal ISSN

Volume Title

Publisher

International Journal of Health Sciences

Abstract

Background: Bisphenol-A is a standard monomer used industrially in manufacturing plastics and epoxy resins, and it is widely used in food preservation and packaging. There is a global increase in its use due to increased demand and the growth in world population. Bisphenol A is an endocrine-disrupting chemical miming the endogenous estradiol hormone. However, due to its exposure to the environment, food and other consumables, its effects on reproductive health have been a growing area of interest. Melatonin regulates sleep-wake cycles and plays essential physiological roles in the body through its antioxidative, anti-cancer and neuroprotective properties. This research aims to ascertain the impact of Bisphenol A on the hypothalamic-pituitary-ovarian axis and determine melatonin's function on possible BPA-induced effects. Methods: Six adult male Wistar rats and 12 adult female Wistar rats of proven fertility were bred and organized into groups. Litters were divided into seven groups, each comprising six rats. These animals were subjected to subcutaneous injections of high and low doses of bisphenol A from postnatal days 0-3, then oral melatonin. The rats were allowed to mature into full-grown adults and euthanized at 120 ±4 days. The serum and hypothalamic-pituitary-ovarian tissues were collected for various assays, histology and genetic studies. Results: Compared to the control groups, groups administered varying doses of bisphenol A showed significant overexpression of estrogen and androgen receptors. Administration of Melatonin showed some reversal and reparative effects on BPA-induced damage of the hypothalamic pituitary ovarian axis. Conclusion: Elevated estrogen receptor levels induced by Bisphenol A altered receptor function, ultimately impairing hormonal cascades that regulate reproductive functions. Melatonin showed some promising reparative effects.

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Keywords

Bisphenol-A, Melatonin, endocrine disruptor, estrogen, Androgen, Receptors

Citation

Kadir, E. R., Yakub, A. D., Imam, A., Olajide, O. J., Lawal, I. A., Oyewole, A. L., Hussein, M. O., Ojulari, L. S., & Ajao, M. S. (2024). Restorative effects of melatonin on bisphenol A-induced interference of gene expression in hypothalamic pituitary axis following early exposure. International Journal of Health Sciences. 8 (S1), 1293–1315. Published by Universidad Technica de Manabi, Ecuador.. Available online at https://sciencescholar.us/journal/index.php/ijhs/issue/view/141

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