Browsing by Author "Kadir, R.E."

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    A new model for alloxan-induced diabetes mellitus in rats.
    (Journal of Bangladesh Society of Physiologists, 2019) Ojulari, L.S.; Oladeru, O.O.,; Ayinde, T.O.; Alade I.O.; Kadir, R.E.; Dangana, O.E.
    Background:Alloxan is widely used to induce experimental diabetes mellitus (DM) in animals with different grades of disease severity by varying the dose of Alloxan used. This method has however be questioned by recent research work as an appropriate technique for the induction of diabetes. Objective: To provide a simple, yet concise and reproducible experimental procedure and model for Alloxan-induced DM in rats. Methods: The study was divided into 2 separate experiments. Experiment 1: Alloxan was administered, into four subgroups each (group 1- 100 mg of Alloxan /kg of rat body weight, group 2- 120 mg/kg, group 3- 150 mg/kg, and group 4- 170 mg/kg); in each subgroup, the dose of Alloxan was administered at different concentrations (20 mg/ml, 10 mg/ml, 5 mg/ml and 4 mg/ml) in groups of 10 rats each. The pre-induction fasting period was also varied between groups. Experiment 2:Following a pre-induction fasting period of 36 hours, animals received 150 mg Alloxan /kg body weight and at a concentration of 20 mg Alloxan/ ml. Result:Alloxan administered intraperitoneally at 150 mg/kg of rat body weight, at 20 mg/ml and following a pre-induction fast period of 36 hours yielded the most favorably conditions with the least recorded mortality. Conclusion: From the results of this study, it can be concluded that alloxan is a diabetogenic drug with a strict protocol of use in inducing a predictable DM in rats and as such, this model is a standard and reproducible technique for the induction of DM in experimental rats.
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    COMPARATIVE ANALYSIS OF HANDGRIP STRENGTH AND URINE C-PEPTIDE CREATININE RATIO AS BIOMARKERS FOR GLUCOSE REGULATION IN YOUNG ADULT FEMALES IN THE UNIVERSITY OF ILORIN: A CROSS-SECTIONAL STUDY
    (Society for Experimental Biology of Nigeria, 2023) Ojulari, L.S.; Sulaiman, S.E.; Ayinde, T.O.; Kadir, R.E.; Hidaayah O. Jimoh-Abdulghaffaar, H.O.; Sulaiman, H.
    Handgrip strength (HGS) is a robust biomarker predicting future disability, metabolic syndrome, and diabetes. Urinary C-peptide creatinine ratio (UCPCR) emerges as a novel, non-invasive tool under exploration for assessing beta cell function and glucose regulation. Despite their significance in gauging muscle strength, mass, and overall metabolic function, gaps remain in understanding the full extent of handgrip strength and UCPCR's efficiency. This study aimed to identify a better biomarker for glucose regulation by studying the relationship between handgrip strength, urine c-peptide creatinine ratio, and blood glucose levels in adult females. Using ELISA, the study measured handgrip strength, blood glucose levels, and urine samples. Social demographic data was obtained through standard questionnaires, and statistical analysis was done using IBM 25 SPSS software with Pearson's correlation, linear regression at P=< 0.05, and T-test. The study found that handgrip strength (HGS) had a slight non-significant positive correlation with fasting blood sugar (FBS) (P=0.386). However, there was a significant correlation between HGS and 2 hours postprandial glucose (2HPG) in both dominant and non-dominant hands (P= 0.045 vs P= 0.017). Additionally, the study found that handgrip strength in the dominant hand was significantly stronger than that in the non-dominant hand (P= 0.001). On the other hand, the urinary C-peptide creatinine ratio (UCPCR) had no significant correlations with FBS and 2HPG. Handgrip strength measurements provide an indicative approach for glucose regulation and are a better biomarker for blood glucose regulation than UCPCR.
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    Prenatal Exposure to Gestational Nicotine before Neurulation is Detrimental to Neurodevelopment of Wistar Rats’ Offspring
    (Penerbit Universiti Sains Malaysia, 2018) Omotoso, G.O.; Kadir, R.E.; Sulaimon, F.; Jaji-Sulaimon, R.; Gbadamosi, I.T
    re before neurodevelopment on the morphology and histology of the prefrontal cortex (PFC) in rats. Methodology: Adult female Wistar rats were time-mated and grouped into three categories: (a) control–given 0.1 mL of normal saline, (b) low-dose nicotine–given 6.88 mg/ kg/d/0.05 mL, and (c) high-dose nicotine–given 13.76 mg/kg/d/0.1 mL in two divided doses. Treatment was given intraperitoneally from gestational days 2 to 6. On postnatal day 15 (P15), the pups were separated from their mothers, anaesthetised and sacrificed, followed by intracardial perfusion with 4% paraformaldehyde. PFC was excised from the brain and processed for tissue histology, histochemistry, and morphology of brain cells. Results: Gestational nicotine exposure during the first week of gestation in rats significantly reduced birth weights in nicotine-treated groups compared with control; it, however, accelerated body weights, altered neuronal morphology, and elevated astrocytic count significantly, while oligodendroglial count was slightly increased in the PFC of juvenile rats examined at P15. Conclusion: These alterations revealed that gestational nicotine exposure before the commencement of the cellular processes involved in brain development negatively affects neurodevelopment, and this could result in neurological dysfunctions in later life.