Browsing by Author "Hamid, A.A.,"

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    Biochemical Properties, In-vitro Antimicrobial and Free Radical Scavenging Activities of the leaves of Annona muricata
    (Faculty of Science, University of Port Harcourt, Nigeria., 2017) Lawal, Z.A.,; Hamid, A.A.,; Shehu A.,; God’shelp E.,; Ajibade O.S.,; Zubair, O.A.,; Ogheneovo, P.,; Mukadam, A.A. &; Adebayo, C.T.
    Fifteen (15) male Wistar rats were completely randomized into three (3) groups. Group A was normal control, Group B received 200 mg/kg weight of ethyl acetate extract of Annona muricata leaves (AMLE) and Group C received 100 mg/kg b.weight AMLE. Single daily dose of AMLE was administered orally for twenty one (21) days after which the animals were sacrificed. The blood samples were taken for haematological and biochemical analysis. Antibacterial and antifungal activities of n-hexane, ethyl acetate and methanol extracts of Annona muricata leaves were carried out. These extracts exhibited satisfactory inhibitory activities against bacterial and fungal strains, which include; Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Klebsiellae pneumonae, Candida albicans, Aspergillus niger, penicillium notatum and Rhizopus stolonifer. N-hexane extract of the plant exhibited antioxidant property by scavenging DPPH radicals with IC50 of 342.44 µg/mL. GC-MS analysis of nhexane, ethyl acetate and methanol extracts of the plant principally revealed the presence of Urs-12-ene (23.15%), Squalene (48.80%) and Clionasterol (15.88%) respectively.
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    Chemical constituents from the leaf extracts of scleria depressa (C.B Clarke) Nelmes with its antioxidant and anti-inflamatory activity.
    (Faculty of Physical Sciences, University of Ilorin., 2018) Oguntoye, S.O.,; Hamid, A.A.,; Jimoh, A.,; Abdulrauf, A.T.,; Bale, M.I.,; Lawal, A.Z.,; Shehu, A.,; Faturoti, I. O.,; Oluwo, A. C. &; Olodu , J. O.
    Antioxidant and antiinflammatory activity of the extracts of Scleriadepressa leaf part were investigated in this study. The leaf part of Scleriadepressa were dried, ground, weighed, and exhaustively extracted with n-hexane, ethylacetate and methanol. GC-MS analysis of the extracts was carried out to know the compounds present in the extracts as well as their molecular formula. These extracts of the plant were evaluated for antioxidant and antiinflammatory activity using peroxide scavenging, lipoxidase and membrane stabilization. Hexane, Ethylacetate and methanol extracts of the Scleriadepressa leaves exhibited antioxidant activity on peroxide radicals at different concentrations ranging from 10-150 µg/mL, using ascorbic acid as standard antioxidant. Ethylacetate and methanol extracts of the plant’s leaves possessed antioxidant activity by exhibiting peroxide free radical scavenging with IC50of 106.23 and 148.79µg/mL respectively, using peroxide antioxidant assay. The hexane extract shows inhibition that is more pronounced compared to that of ethylacetate for the antiinflammatory activity while methanol extract of the plant’s leaves shows activity higher than that of hexane and ethylacetate for the anti-inflammatory activity. The GC-MS analysis shows the presence of 13 compounds for hexane extract with Carvomenthol,( an α-Terpenol) and 1,2,3-Trimethylbenzene being the abundant compounds with %abundanceof 23.78% and 19.20% respectively, while ethylacetate extract revealed 19 compounds with 1,2- Benzenedicarboxylic acid,bis(2-methylpropylester), and 4,7-dimethylundecane with corresponding % abundance of 24.56% and 16.83% being the abundant compound also the GC-MS analysis of methanol extract of Scleriadepressa leaves showed the presence of 11 compounds. The compound with highest abundance is Methyl9-octadecenoate, with %abundance of 69.86 and retention time of 14.714.
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    Thaumatococcus danielli leaves; It’s Chemical composition, antioxidant and antimicrobial activities.
    (Faculty of Science Obafemi Awolowo University, Ile-Ife, Nigeria., 2017) Hamid, A.A.,; Aliyu, M.A.,; Lawal, A.Z,; Mukadam A.A.,; Shehu A. &; Egharevba, G.
    The preliminary phytochemical investigation of n-hexane, ethyl acetate and methanol extracts of Thaumatococcus daniellii leaves revealed the presence of fats and oils, terpenoids, flavonoids, steroids and glycosides. The antimicrobial tests against some strains of bacteria and fungi showed inhibitions at moderate to high concentrations. Methanol extract of the plant exhibited low 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity with IC of 615.14 μg/ml. Gas chromatography-mass spectrometry (GC-MS) 50 characterization of n-hexane, ethylacetate and methanol extracts of T. daniellii leaves identified , thirteen and fifteen compounds, with tetracontane (28.76%) and L-ascorbic acid (15.07%); hexadecanoic acid (21.62%) and γ-sitosterol (11.06%); and naphthalene-1,2,3,5,6,7,8,8a-octahydro-1,8a-dimethyl-7-(1-methylethenyl)- ,[1R(1.alpha.,7.beta.,8a.alpha.] (26.90%) and hexadecanoic acid (12.60%) being the major compounds respectively. The GC-MS analysis revealed various peaks of bioactive compounds of which the antioxidant and antimicrobial activities of the plant have been attributed to the prominent compounds in synergy with all the other compounds present in smaller quantities in the extracts.