Chemical constituents from the leaf extracts of scleria depressa (C.B Clarke) Nelmes with its antioxidant and anti-inflamatory activity.
No Thumbnail Available
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Faculty of Physical Sciences, University of Ilorin.
Abstract
Antioxidant and antiinflammatory activity of the extracts of Scleriadepressa leaf part were investigated in this
study. The leaf part of Scleriadepressa were dried, ground, weighed, and exhaustively extracted with n-hexane,
ethylacetate and methanol. GC-MS analysis of the extracts was carried out to know the compounds present in the
extracts as well as their molecular formula. These extracts of the plant were evaluated for antioxidant and
antiinflammatory activity using peroxide scavenging, lipoxidase and membrane stabilization. Hexane,
Ethylacetate and methanol extracts of the Scleriadepressa leaves exhibited antioxidant activity on peroxide
radicals at different concentrations ranging from 10-150 µg/mL, using ascorbic acid as standard antioxidant.
Ethylacetate and methanol extracts of the plant’s leaves possessed antioxidant activity by exhibiting peroxide free
radical scavenging with IC50of 106.23 and 148.79µg/mL respectively, using peroxide antioxidant assay. The
hexane extract shows inhibition that is more pronounced compared to that of ethylacetate for the antiinflammatory activity while methanol extract of the plant’s leaves shows activity higher than that of hexane and
ethylacetate for the anti-inflammatory activity. The GC-MS analysis shows the presence of 13 compounds for
hexane extract with Carvomenthol,( an α-Terpenol) and 1,2,3-Trimethylbenzene being the abundant compounds
with %abundanceof 23.78% and 19.20% respectively, while ethylacetate extract revealed 19 compounds with 1,2-
Benzenedicarboxylic acid,bis(2-methylpropylester), and 4,7-dimethylundecane with corresponding % abundance
of 24.56% and 16.83% being the abundant compound also the GC-MS analysis of methanol extract of
Scleriadepressa leaves showed the presence of 11 compounds. The compound with highest abundance is Methyl9-octadecenoate, with %abundance of 69.86 and retention time of 14.714.