Browsing by Author "Garuba, T."

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    Comparative biodiversity assessment of weed species in monocropping plantations of University of Ilorin, Nigeria.
    (Institute for Enviroment and Sanitation Studies, University of Ghana, Legon, 2020) Olayinka, U.B.; Adeyemi, S.B.; Abdulkareem, K.A.; Olahan, G.S.; Lateef, A.A.; Garuba, T.; AbdulRahaman, A.A.
    The present study investigates the weed species diversity in four plantations of university of Ilorin, Ilorin, Nigeria using quadrat method. The survey revealed two major life cycles (annual and perennial) and recorded a total of 88 weed species belonging to 32 families. Four species (Azadirachta indica, Daniellia oliveri, Desmodium tortuosum, and Tridax procumbens) were common in all the surveyed plantations while the family Fabaceae was the most dominant. The abundant weed species analysis showed a high importance value index and were more adapted to the plantations. Diversity analysis revealed high species richness in the sugarcane plantation. The non-canopy nature of the plantation, soil structure as well as ability to coexist with many other species may underscore the reasons for this pattern of diversity. The evenness and similarity indices between and across the plantations were generally low, thus, indicating varying diversity. As a result of the recorded variation in weed composition between and across the plantations, the study has provided an insight on the pattern of weed diversity in the studied plantations. The study recommended that the most abundant weed species populations be checked for the plantations to thrive. Finally, there is an urgent need to conserve weed species that are not only rare in abundance but also showed great social and economic values.
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    EFFECT OF SODIUM AZIDE ON MORPHOLOGICAL CHARACTERS OF THREE TOMATO ACCESSIONS (SOLANUM LYCOPERSICON L.)
    (Institute of Biology, Bucharest, Romania, 2017) Abdulkareem, K.A; Garuba, T.; Akande, E.O; Mustapha, O.T
    The effect of chemical mutagen (Sodium azide) was used to study the morphological characters of three accessions of tomato (Solanum lycopersicon) and to estimate the heritability of treated plants. Dry seeds of one accession (NGB01302) were collected from National Centre for Genetic Resources (NACGRAB) and two accessions (Tropimech and Tima) were collected from National Horticultural Research Institute (NIGHORT), Ibadan, Nigeria. The seeds were presoaked in water to test for viability. The seeds were soaked in sodium azide of concentrations 1mM, 2mM and 4mM in phosphate buffer of pH 4 for 4 hours and rinsed under a running tap to remove excess mutagen. The result of the study showed that the control of Tropimech had the highest germination (100%) while the lowest was recorded in treatment with 1mM. At four weeks of planting (4WAP), there was no significant difference between the heights of treated seedlings of Tropimech while the untreated seedlings were significantly different from the treated seeds. No significant difference was observed in both treated and untreated seedlings of Tima and NGB01302. Highly significant differences (P<0.05) were observed in the number of leaves, number of branches, number of flowers, number of fruit and fruit weight in the three accessions. The heritability of the yield parameters (number of flower and fruit weight) were moderate and the genetic advance was high in the number or leaves, number of flowers and fruit weight. There was general reduction in all parameters under study with increasing concentration of sodium azide except in NGB01302 which showed an increase.
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    Molecular Characterisation of Some Accessions of Corchorus olitorius l.
    (Faculty of Science, Federal University Birnin Kebbi, 2019-12) Abdulkareem, K.A; Sidiq, K.A; Olayinka, B.U.; Lateef, A.A.; Garuba, T.; Olahan, G.S.; Adeyemi, S.B.; Sagaya, A.; Tiamiyu, B.B.; Abdulrahman, A.A.
    Nigeria has several native leafy vegetables that have been neglected. Corchorus olitorius belongs to this group. Genetic diversity was studied in five accessions of the plant using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS - PAGE). The aim was to determine the relationship (differences and similarities) among them. Sixteen polypeptide bands were obtained ranging from 12.29 KDa to 170 KDa indicating variations in the banding patterns of the accessions. The unweighted pair group method with arithmetic algorithm (UPGMA) dendrogram grouped the five accessions into three clusters with four groups showing 60% of the accessions in one group. The greatest similarity (96%) observed was between NGB00196 and NGB00194 while the lowest similarities (32%) NGB00191 and (40%) NGB00187. Adopting this technology can be useful in plant variety identification and registration of new plant varieties. NGB00191 was observed to be distantly related to the other accessions hence could be combined in a breeding programme.
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    Molecular characterization of potential crop pathogens associated with weeds as endophytes in Unilorin plantations, Nigeria.
    (College of Science for Women, University of Baghdad, Jadriah, Baghdad, Iraq., 2022-05-20) Lateef, A.A.; Garuba, T.; Abdulkareem, K.A.; Olayinka, B.U.; Olahan, G.S.; Adeyemi, S.B.; AbdulRahaman, A.A.
    Crop diseases are usually caused by inoculum of pathogens which might exist on alternate hosts or weeds as endophytes. These endophytes, cum pathogens, usually confer some beneficial attributes to these weeds or alternate hosts from protection against herbivores, disease resistance, stress tolerance to secondary metabolites production. This study was therefore carried out to isolate potential crop pathogens which exist as endophytes on weed species in the University of Ilorin plantations. Green asymptomaticleaves were collected from 10 weed species across the plantations, and processed for their endophytic fungi isolation. Isolates were purified into pure cultures and used for molecular identification using the internal transcribed spacer (ITS) region of the ribosomal DNA. Phylogenetic analysis of the fungal sequences using MEGA software revealed 9 fungal genera belonging to 13 species, with species in the genera Curvularia, Epicoccumand Daldiniaoccurring in more than one weed species, while other genera such as Alternaria, Fusarium, Chaetomium, Macrophomina, Arthriniumand Phomopsisoccurred in just one weed species each. Daldinia eschscholtziiwas isolated in this study as an endophyte from Loudetia arundinaceafor the first time. This plant is very abundant in Nigeria and Africa where it is used majorly for thatching and feeding livestocks. This also represents the first endophytic fungi from the genus Loudetia. Potential relationship between the occurrences of these fungi as endophytes and as pathogens are discussed. These discoveries represent the first large-scale molecular identification and several first reports of endophytes from these weed species. These results also represent the first records of some of these fungi in Nigeria.
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    PHYTOCHEMICAL SCREENING, ANTI-OXIDANT AND ANTI-MICROBIAL ACTIVITIES OF FRACTIONATED ETHANOLIC EXTRACT OF Moringa Oleifera LEAVE
    (Chemical Society of Nigeria, 2021) Ameen, O. M.; Akinolu, A. A.; Garuba, T.; Ebito, G. E.; Arowolo, B. Z.; Oyebanji, E. O.
    Moringa oleifera leave has been reputed for the treatment of different ailments, this work thus aims at isolating the active principle(s) that are responsible for these activities. M. oleifera leave was extracted with distilled ethanol, concentrated to afford the crude extract (210.2 g). The crude extract was partitioned using n – hexane (NH), dichloromethane (DCM), ethyl acetate (EA) and methanol (MeOH) in an increasing order of polarity to afford 12 fractions. The fractions were subjected to biological testing and MOF8 and MOF11 were selected for further purification based on their antioxidant and antimicrobial activities. The two fractions (MOF8 and MOF11) were purified on a silica gel open column, using NH, DCM, EA and MeOH in an increasing order of polarity to afford ten and eight fractions respectively. Further biological test on these fractions indicates superior activities of MOF118 which was further purified as described earlier to afford four fractions. Further biological testing on these fractions indicates that and MOF118.3 and MO118.4 are more active in the antioxidant and antimicrobial analysis. Phytochemical screening of MOF11 and MOF11 indicates the presence of flavonoids, saponin, tannins, alkaloids, glycosides and steroids.
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    Rhizosphere and non - rhizosphere soil mycoflora of corchorus olitorus
    (Faculty of Science, Kaduna State University., 2016) Olahan, G.S; Sule, I.O.; Garuba, T.; Salawu, Y.A.
    The physicochemical and microbial analyses of the rhizosphere and non-rhizosphere soils of Corchorus olitorius (Jute) were conducted. The soil samples were analyzed before planting of Jute seeds and the average values of the parameters were 11.24% (percentage moisture content), 0.29ml/g (water holding capacity), 1.36% (organic matter content) and 6.80 (soil pH). The textural class of the soil sample is sandy. Aspergillus niger and Rhizopus stolonifer occurred most followed by Aspergillus flavus, while Penicillium chrysogenum, Saccharomyces cerevisiae and Neurospora crazza occurred least in the rhizosphere and non – rhizosphere soil samples of Jute in this study. Occurrence of these fungi in both the rhizosphere and non-rhizosphere soils varied widely throughout the duration of this study. It can be concluded that the jute plants benefitted from the microorganisms (fungi) in their rhizosphere and non – rhizosphere zones since the plants showed healthy growth.