EFFECT OF SODIUM AZIDE ON MORPHOLOGICAL CHARACTERS OF THREE TOMATO ACCESSIONS (SOLANUM LYCOPERSICON L.)
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Date
2017
Authors
Abdulkareem, K.A
Garuba, T.
Akande, E.O
Mustapha, O.T
Journal Title
Journal ISSN
Volume Title
Publisher
Institute of Biology, Bucharest, Romania
Abstract
The effect of chemical mutagen (Sodium azide) was used to study the morphological characters of three accessions of tomato (Solanum lycopersicon) and to estimate the heritability of treated plants. Dry seeds of one accession (NGB01302) were collected from National Centre for Genetic Resources (NACGRAB) and two accessions (Tropimech and Tima) were collected from National Horticultural Research Institute (NIGHORT), Ibadan, Nigeria. The seeds were presoaked in water to test for viability. The seeds were soaked in sodium azide of concentrations 1mM, 2mM and 4mM in phosphate buffer of pH 4 for 4 hours and rinsed under a running tap to remove excess mutagen. The result of the study showed that the control of Tropimech had the highest germination (100%) while the lowest was recorded in treatment with 1mM. At four weeks of planting (4WAP), there was no significant difference between the heights of treated seedlings of Tropimech while the untreated seedlings were significantly different from the treated seeds. No significant difference was observed in both treated and untreated seedlings of Tima and NGB01302. Highly significant differences (P<0.05) were observed in the number of leaves, number of branches, number of flowers, number of fruit and fruit weight in the three accessions. The heritability of the yield parameters (number of flower and fruit weight) were moderate and the genetic advance was high in the number or leaves, number of flowers and fruit weight. There was general reduction in all parameters under study with increasing concentration of sodium azide except in NGB01302 which showed an increase.
Description
Keywords
Sodium azide, Mutagen, Solanum lycopersicon, accession
Citation
Abdulkareem, K. A., Garuba, T., Akande, E.O. & Mustapha, O.T. (2017). Effect of sodium azide on morphological characters of three tomatoes accessions (Solanum lycopersicon L.). Romania Journal of Biology- Plant Biology, 62(2): 109 – 117