Molecular Characterisation of Some Accessions of Corchorus olitorius l.
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Date
2019-12
Journal Title
Journal ISSN
Volume Title
Publisher
Faculty of Science, Federal University Birnin Kebbi
Abstract
Nigeria has several native leafy vegetables that have been neglected. Corchorus olitorius belongs to this group. Genetic diversity was studied in five accessions of the plant using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS - PAGE). The aim was to determine the relationship (differences and similarities) among them. Sixteen polypeptide bands
were obtained ranging from 12.29 KDa to 170 KDa indicating variations in the banding patterns of the accessions. The unweighted pair group method with arithmetic algorithm (UPGMA) dendrogram grouped the five accessions into three clusters with four groups showing 60% of the accessions in one group. The greatest similarity (96%) observed was between NGB00196 and NGB00194 while the lowest similarities (32%) NGB00191 and (40%) NGB00187. Adopting this technology can be useful in plant variety identification and registration of new plant varieties. NGB00191 was observed to be distantly related to the other accessions hence could be combined in a breeding programme.
Description
Keywords
Diversity, Corchorus olitorius, Genetics, Electrophoresis, Protein
Citation
Abdulkareem, K.A., Sidiq, K.O., Olayinka, B.U., Lateef, A.A., Garuba, T. & Olahan, G.S. (2019): Molecular characterisation of some accessions of Corchorus olitorius L. Savanna Journal of Basic and Applied Sciences, 1(2); 213-217