Browsing by Author "Babatunde, A.S."
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Item Acute and Sub-acute Toxicities of Defatted Ethanolic Extract of Moringa Oleifera seed in Albino Rats(West African Society for Pharmacology, 2015) Olorundare, O.E.; Bello, M.K.; Biliaminu, S.A.; Babatunde, A.S.; Ibrahim, O.O.K.; Anoka, A.A.Item Antimalarial Activity of Cocos nucifera Husk fibre: Further Studies(Hindawi Publishing Corporation, 2013) Adebayo, J.O.; Balogun, E.A.; Malomo, S.O.; Soladoye, A.O; Olatunji, L.A.; Kolawole, O.M.; Oguntoye, O.S.; Babatunde, A.S.; Akinola, O.B.; Aguiar, A.C.C; Andrade, I.M.; Souza, N.B.; Krettli, A.U.Abstract In this study, the antimalarial and toxicity potentials of husk fibre extracts of five Nigerian varieties of Cocos nucifera were evaluated in vitro. The only active extract fraction, West African Tall (WAT) ethyl acetate extract fraction, was then evaluated for its phytochemical constituents, antimalarial and toxicity potentials at varying doses (31.25–500 mg/kg body weight) using various organ function indices. The results revealed that WAT ethyl acetate extract fraction (WATEAEF) contained alkaloids, tannins, and flavonoids and was active against Plasmodium falciparum W2 strain maintained in continuous culture, with a selectivity index of 30.3. The same extract fraction was active in vivo against Plasmodium berghei NK65, causing more than 50% reduction in parasitaemia on days 4 and 6 after inoculation at various doses administered. WATEAEF did not significantly alter ( ) function indices of the liver and cardiovascular system at all doses administered but significantly increased ( ) plasma creatinine concentration at 250 and 500 mg/Kg body weight compared to controls. The results of this study suggest that WATEAEF possesses antimalarial activity and may not adversely affect normal liver function nor predispose subjects to cardiovascular diseases but may impair normal kidney function at higher doses. Further studies are underway to isolate the active principles.Item Atypical chronic myeloid leukaemia in an adolescent nigerian: a case report and review of the literature(College of Medicine, Ambrose Alli University, 2017) Babatunde, A.S.; Olawumi, H.O.; Durotoye, I.A.; Shittu, A.O.; Adepoju, A.M.Abstract: Atypical chronic myeloid leukaemia (aCML) is a rare subtype of CML which is now regarded as a separate clinical entity and classified among the Myeloproliferative/myelodysplasic syndromes. It lacks the Philadelphia chromosome and BCR-ABL fusion gene found in the classical CML. We report a case of aCML which was diagnosed and managed at the Haematology Department of the University of Ilorin Teaching Hospital, Ilorin. Full blood count showed anaemia, leucocytosisand presence of various forms of myeloid cells with dysplastic features. Bone marrow aspiration done also showed myeloid hyperplasia with dysplastic changes in the myeloid cells. There was poor outcome in the patient. This case illustrates the importance of full haematological investigations of suspected CML cases so that the diagnosis of a CML will not be missed or passed for CML as this could influence choice of treatment and prognosis of patients. There is need for improved diagnostic facilities in tertiary health institutions in the country if we must achieve proper diagnosis and characterization of cases of aCML.Item Autologous blood donations and transfusions among patients undergoing elective orthopaedic, gynaecologic and elective caesarean section at a Tertiary Hospital in North Central Nigeria(Kenya Medical Association, 2018) Shittu, A.O.; Olawumi, H.O.; Adesina, K.T.; Babalola, O.M.; Ibraheem, G.H.; Babatunde, A.S.; Olatinwo, A.W.O.ABSTRACT Introduction: Allogeneic donor blood is becoming increasingly costly, scarce and associated with multiple risks; there is need for more conservative transfusion strategies, one of which may be adoption of Autologous Blood Donation and Transfusions (ABDT). Despite increased acceptance of ABDT by clinicians and patients in most developed world, awareness in this part of the world is still low. Materials and methods: this is a cross sectional survey of knowledge and acceptance of ABDT by patients scheduled for elective orthopaedic, gynaecologic and elective caesarean section surgeries using structured interviewer administered questionnaire on socio-demographics, clinical presentations, knowledge of blood donations and transfusions, and acceptance of ABDT. The study population comprised of 171 subjects. Results: Gynaecologic/obstetric patients were 120 (70.2%) while 51 (29.8%) of the subjects were Orthopaedic patients. Majority (43.95%) were within the age range 31-45 years, 38.6% were less than 31 years while 17.5% were older than 45 years. Females constituted the larger proportion (77.2%). Twenty one (12.3%) subjects had donated blood in the past, 45 (26.3%) had previous blood transfusions and 30 (17.5%) had knowledge of ABDT. Eight one (47.4%) of all subjects accepted ABDT for the scheduled surgeries while 123 (71.9%) subjects accepted to be voluntary blood donors after surgery. Pre-deposit is the commonest form of ABDT preferred by the subjects (66%), followed by Haemodilution (28%) and Cell salvage (6%). February 2018 EAST AFRICAN MEDICAL JOURNAL 1215 significant number of the subjects who accepted ABDT were ready to become voluntary blood donors after surgery (90.1%). Conclusion: Acceptance rate as high as 47.4% was recorded in this study despite low awareness. This is higher than what was reported by most authors’ locally but similar to the findings from Europe and Asia. Pre-deposit form was the commonest ABDT acceptable to these patients. Improving awareness on ABDT will boost voluntary blood donations and improve blood transfusion safety.Item Changing pattern of haematological malignancies in Ilorin, Nigeria: A 10 year retrospective review of 181 cases(Ambrose Alli University, 2016) Babatunde, A.S.; Olawumi, H.O.; Durotoye, I.A.; Shittu, A.O.; Akinwumi, O.O.Abstract The global burden of cancer has been reported to be on the increase and haematological malignancies (HM) are not exceptions to this observed trend. The aim of this study was to carry out a retrospective analysis of all cases of HM seen at the University of Ilorin Teaching Hospital, Ilorin and document the current pattern of HM and compare with previous reports from Nigeria and elsewhere. Materials used for the study were obtained from the Records Cancer Registry of the University of Ilorin Teaching Hospital. A total of 181 cases of HM were diagnosed and managed within the study period constituting 8.1% of all cancer cases and 0.2% of all hospital admissions. The ages of patients with HM ranged from 7 months to 80 years. There were 116 (64.1%) males and 65 (35.9%) females. The various HM recorded in the study were: ALL 17(9.4%), AML 23(12.7%), CLL 33(18.2%), CML 46(25.4%), NHL 33(18.2%), HL 7(3.9%), BL 2(1.1%) and MM 20(11.0%). CML was the commonest HM observed in this study. NHL was the commonest lymphoma encountered constituting 82.5% of all the lymphoma cases. This study demonstrated that haematological malignancies are common in this environment. Availability of improved diagnostic facilities, improved blood transfusion services, early presentation and better referral system from other health facilities to our centre may have contributed to the improved mean survival of patients with HM observed in this study.Item Characterization of hemopoietic engraftment kinetics and development of secondary cytopenia in AML post Auto-SCT and its correlation with survival outcome(Nature Publishing Group, 2009) Babatunde, A.S.; Tan, D.C.L; Heng, K.K; Lee, J.J; Loh, Y.S.M; Goh, Y.T; Linn, Y.CWe performed a single center retrospective analysis of 84 autologous hemopoietic stem cell transplants done for AML to characterize the pattern of hemopoietic engraftment, post-transplant cytopenia and their impact on survival outcome. Following autologous transplant and engraftment, 30 patients (35.7%) had a transient secondary decline in their plt counts, which was not associated with graft rejection, relapse or infection. The median time to onset of thrombocytopenia was 59 days post transplant, with spontaneous recovery after a median period of 41 days. A secondary decline in ANC also occurred in eight patients. Patients with secondary plt decline had a significantly earlier primary plt engraftment (median 15 days) and a trend towards earlier neutrophil engraftment compared with patients who maintained steady plt counts (median 21 days). There was a trend towards a lower incidence of secondary plt decline in patients who received BM stem cells compared with those who received PBSC. No cause was evident for the occurrence of a secondary cytopenia, and it did not adversely affect survival. We conclude that secondary cytopenia is a common and harmless occurrence after autologous transplant especially from PBSC graft. Bone Marrow Transplantation (2009) 44, 175–183; doi:10.1038/bmt.2009.1; published online 9 February 2009 Keywords: acute myeloid leukemia; autologous hemopoietic stem cell transplant; engraftment kinetics; secondary platelet decline; secondary cytopeniaItem The Chest Radiographs of Suspected and Confirmed HIV/AIDS Patients: Pattern of Radiological Features in Ilorin(College of Health Sciences, University of Ilorin, 2008) Oguntoyinbo, A.E.; Nzeh, D.A.; Salami, A.K.; Babatunde, A.S.Item Chitosan-based nanoformulated (–)-epigallocatechin-3-gallate (EGCG) modulates human keratinocyte-induced responses and alleviates imiquimod-induced murine psoriasiform dermatitis(Taylor & Francis, 2018) Chamcheu, J.C.; Siddiqui, I.A.; Adhami, V.M.; Esnault, S.; Bharali, D.J.; Babatunde, A.S.; Adame, S.; Massey, R.J.; Wood, G.S.; Longley, B.J.; Mousa, S.A., Mukhtar, H.; Mousa, S.A.,; Mukhtar, H.Background: Psoriasis is a chronic and currently incurable inflammatory skin disease characterized by hyperproliferation, aberrant differentiation, and inflammation, leading to disrupted skin barrier function. The use of natural agents that can abrogate these effects could be useful for the treatment of psoriasis. Earlier studies have shown that treatment of keratinocytes and mouse skin with the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) mitigated inflammation and increased the expression of caspase-14 while promoting epidermal differentiation and cornification. However, bioavailability issues have restricted the development of EGCG for the treatment of psoriasis. Materials and methods: To overcome these limitations, we employed a chitosan-based polymeric nanoparticle formulation of EGCG (CHI-EGCG-NPs, hereafter termed nanoEGCG) suitable for topical delivery for treating psoriasis. We investigated and compared the efficacy of nanoEGCG versus native or free EGCG in vitro and in an in vivo imiquimod (IMQ)-induced murine psoriasis-like dermatitis model. The in vivo relevance and efficacy of nanoEGCG formulation (48 μg/mouse) were assessed in an IMQ-induced mouse psoriasis-like skin lesion model compared to free EGCG (1 mg/mouse). Results: Like free EGCG, nanoEGCG treatment induced differentiation, and decreased proliferation and inflammatory responses in cultured keratinocytes, but with a 4-fold dose advantage. Topically applied nanoEGCG elicited a significant (p,0.01) amelioration of psoriasiform pathological markers in IMQ-induced mouse skin lesions, including reductions in ear and skin thickness, erythema and scales, proliferation (Ki-67), infiltratory immune cells (mast cells, neutrophils, macrophages, and CD4+ T cells), and angiogenesis (CD31). We also observed increases in the protein expression of caspase-14, early (keratin-10) and late (filaggrin and loricrin) markers of differentiation, and the activator protein-1 factor (JunB). Importantly, a significant modulation of several psoriasis-related inflammatory cytokines and chemokines was observed compared to the high dose of free EGCG (p,0.05). Taken together, topically applied nanoEGCG displayed a .20-fold dose advantage over free EGCG. Conclusion: Based on these observations, our nanoEGCG formulation represents a promising drug-delivery strategy for treating psoriasis and possibly other inflammatory skin diseases. Keywords: chitosan nanoparticles, topical delivery of chitosan nanoformulated EGCG, psoriasis-like skin inflammation, phytochemical treatment of psoriasis, normal human epidermal keratinocytes, differentiation, anti-inflammatory actionItem Clinico-Laboratory Features of Multiple Myeloma in Selected Patients at University of Ilorin Teaching Hospital, North Central Nigeria(University of Ilorin, 2019) Babatunde, A.S.; Durotoye, I.A.; Shittu, A.O.; Omokanye, K.O.; Ogunfemi, M.K.; Sanni, E. O.; Owoeye, O.A.Abstract Multiple myeloma is an haematological disorder characterized by clonal proliferation of bone marrow plasma cells with production of increased amounts of monoclonal immunoglobulins which are detectable in blood and/or urine. The aim of the study was to describe the clinical and laboratory characteristics as well as treatment outcome in multiple myeloma patients diagnosed and managed at the Haematology Department of the University of Ilorin Teaching Hospital (U.I.T.H), Ilorin, North Central Nigeria, between 1st January 2008 and 31st December 2018. The study materials included bone marrow registers from the department and case folders of all multiple myeloma patients diagnosed within the study period. Relevant data including socio-demographic characteristics of patients, clinical features at presentation and laboratory tests results were extracted, documented and analyzed. Diagnosis was made in all patients based on the presence of major and/or minor criteria as described by the International Myeloma Working Group guidelines. Fifty eight (58) patients were diagnosed with multiple myeloma comprising of 31 males and 27 females (M:F ratio = 1.15:1). The median age of patients was 62.6 years (range = 39 – 85 years), and the most common presenting features included back pains (86.2%), bone pains (69%), inability to walk (67.2%) and anaemia (67.2%). High erythrocyte sedimentation rate > 30mm/Hr was seen in all patients (100%), Immunoglobulin G (Ig G) was the commonest monoclonal immunoglobulin demonstrated on serum protein electrophoresis (82.8%), and “punched out” osteolytic bone lesion on X-rays was demonstrated in 79.3% of cases. In resource-limited settings such as ours, the diagnosis and monitoring of patients with multiple myeloma still rely on the clinical and laboratory features of the disease to a large extent, hence the need for haemato-oncologists and physicians to be conversant with these features.Item Clinico-Pathologic features and outcome of Chronic Lymphocytic Leukemia in Ilorin, North Central Nigeria(College of Health Sciences, University of Ilorin, 2020) Babatunde, A.S.; Durotoye, I.A.; Shittu, A.O.; Omokanye, K.O.; Ogunfemi, M.K.; Owoeye, O.A.; Sanni, E.O.Abstract: A retrospective analysis of all patients that were diagnosed and managed for Chronic lymphocytic Leukemia(CLL) in the Department of Haematology of University of Ilorin Teaching Hospital, Ilorin between 1st January 2005 and 31st December 2018 was carried out with the aim of characterizing the clinical, haematological and prognostic features as well as outcome in the patients. A total of 39 patients were reviewed consisting 25(64.1%) males and 14(35.9%) females giving a M:F ratio of 1.6:1. The median age of patients at diagnosis was 65.5 years and the highest prevalence was found in the 60 – 69 years age group. The commonest symptoms at presentation were night sweats (59.0%), Body weakness (51.3%), fever (41.0%), and weight loss (41.0%) while the commonest signs included lymphadenopathy in all patients (100%), splenomegaly (87.2%), pallor (53.9%) and hepatomegaly (46.2%). The mean values of haematological parameters were: Packed cell volume (28.6%), Haemoglobin concentration (9.4g/dL), Total white blood cell count (87.6 x 109/L), Absolute lymphocyte count (55.8 x 109/L) and platelet count (134.8 x 109/L). The majority of the patients (94.9%) were diagnosed with advanced disease (Rai stage III and IV) while only 2(5.1%) had Rai stage II and none in Rai stage 0 – I. Chlorambucil-based regimen was used in 71.8% of patients, and only 2 patients had Rituximab as part of their treatment regimen. The clinical presentation, haematological features and disease outcome observed in our study was similar to what had been reported in Nigeria and other parts of the world.Item Clinicopathological features of chronic myeloid leukemia at diagnosis: Study of a series of 46 cases(Ibn Sina Medical College, 2016) Shittu, A.O.; Babatunde, A.S.; Adewoye, A.O.Introduction: A variety of symptoms and signs are said to be common at diagnosis of chronic myeloid leukemia, but their exact incidence is not well documented. There are conflicting opinions on the incidences of the symptoms and signs. Subjects and methodology: This is a retrospective study whereby the clinical and laboratory features of 46 patients diagnosed as chronic myeloid leukemia at different phases between 1999 and 2009 were retrieved from their case files and analyzed. Results: Of all the patients, 38, 6 and 2 presented in chronic, accelerated and blastic phases respectively. The mean age of the series was 38.3 years (range 17-68 years). The peak age of presentation was 31-40 years (30.48%) followed by 21-30 years (26.1%), 41-50 (21.7%), above 50 years (17.4%) and 10-20 years (4.3%). There was a slight male preponderance 24:22 (1.09:1) with 65% of patients being married and 35% single. Occupation wise, the ratio of petrochemical and benzene related jobs to others was 3:43 (0.07:1). Spleneomegally was the commonest presenting clinical feature in this series and was reported in 44 (95.6%) of our patients. Others were anemia, weight loss, fever, hepatomegally and night sweat. Conclusion: Because of unavailability and unaffordability of the sophisticated diagnostic tools like quantitative PCR in developing worlds, there is need for clinicians to be up to date with the usual and common clinical and laboratory features of chronic myeloid leukemia.Item Comparative Analysis of Baseline and One Year Post HAART Haematological Parameters in HIV Infected Patients in Ilorin, Nigeria(Sikkim Manipal University, 2017) Olawumi, H.O.; Babatunde, A.S.; Adepoju, A.M.; Omokanye, K.O.; Jayeola, A.A.; Ogunfemi, M.K.; Oladosu-Olayiwola, O.R.Abstract:Haematological abnormalities are common manifestations of HIV infection. The aim of this study was to determine the effect of HAART on haematological parameters of HIV infected patients.This was a longitudinal prospective study conducted at the HAART clinic of University of Ilorin Teaching Hospital, Ilorin. Eighty newly diagnosed HIV positive patients between 21-75 years who were planned to commence HAART (lamivudine, zidovudine and nevirapine) were recruited into this study. Baseline and one year post- HAART full blood count were carried out by Sysmex KX21 according to manufacturer’s instructions.Twenty nine percent of the patients had anaemia, 13% had neutropaenia and 0.02% had thrombocytopaenia at presentation. There was a significant increase in mean hemoglobin concentration of the patients from 10.79±1.89g/dl at baseline to 11.52±1.27g/dl at one year post HAART initiation. (P value=0.003) There was a statistically significant difference in mean baseline haemoglobin concentration of patients in WHO clinical stages 1, 2 and 3(11.67±1.39g/dl, 10.47±2.09g/dl, 10.00±1.86g/dl respectively; P=0.012). HIV patients were more likely to present with anaemia followed by neutropaenia and rarely thrombocytopaenia. Severity of anaemia increased with disease progression. HAART led to improvement in haematological abnormalities with significant increase in haemoglobin concentration. Haemoglobin concentration may therefore be used to monitor response to HAART in HIV infected patients.Item Current Trend of Transfusion Transmissible Infections among Voluntary Blood Donors in North Central Nigeria(College of Health Sciences, University of Ilorin, 2021-07) Durotoye, I.A.; Salaudeen, A.G.; Sanni, E.O.; Durowade, K.A.; Babatunde, A.S.; Olawumi, H.O.; Akande, T.M.; Musa, I.O.Blood transfusion is an integral part of modem medicine which had saved many lives which would have been lost as a result of lack of blood. Although blood is needed for survival, it can also be a medium for the transmission of harmful infectious diseases. This study evaluated current trend of transfusion transmissible infections among voluntary blood donors in Nigeria. This was a descriptive community-based study involving 600 prospective voluntary blood donors among staff and students of 5 tertiary institutions in Kwara and Kogi States. Hepatitis B surface antigen and Hepatitis C antibodies were screened for using Hepatitis B Virus Surface Antigen Bio-Inteco ELISA KIT, and Anti-HCV Bio-Inteco ELISA KIT, respectively. Screening for HIV was done with Enzyme immunoassay method using Human Immunodeficiency Virus (HIV) 1+2 Antigen Antibody ELISA KIT, product of Bio-Inteco HIV Diagnostics, UK. Testing for Syphilis was carried out using Rapid Plasma Reagin (RRR) test. Statistical analysis was done with IBM SPSS version 20. Level of significance was set at p< 0.05 The mean age of the subjects was 21.98 ± 5.17 years. Two thirds of the prospective blood donors were within the second decade of life and more than half were males. The prevalence of Hepatitis B among subjects were 4.7%, Hepatitis C,0.2%, HIV 1% and Syphilis 0%. The prevalence of hepatitis B and HIV were relatively high compared to Hepatitis C and Syphilis among voluntary blood donors, although lower than the prevalence among general population in Nigeria. In order to improve on blood transfusion safety, there is a need to increase public awareness on vaccination for hepatitis B virus, Quality control of screening methods and rational use of Blood.Item Evaluation of Antimalarial and Toxicity Potentials of Methanolic fraction of cocos nucifera (West African Tall variety) husk fibre extract in animal models(Parasitology and Public Health Society of Nigeria, 2013-03) Ugbomoiko, U.S.; Awoniyi, M.A.; Balogun, E.A.; Malomo, S.O.; Soladoye, A.O.; Adebayo, J.O.; Kolawole, O.M.; Oguntoye, O.S.; Olatunji, L.A.; Babatunde, A.S.; Akinola, O.B.The antimalarial and toxicity potentials of the methanolic fraction of Cocos nucifera (West African tall variety) husk fibre extract were investigated using animal models. For the 4-day suppressive antimalarial test, thirty Plasmodium berghei NK65-infected mice were randomly divided into six groups (A-F) with five mice each. Mice in group A (control) received orally appropriate volume of distilled water while those in group B were orally administered chloroquine (20 mg/Kg body weight) for three days post-inoculation. Mice in groups C-F were orally administered 62.5, 125, 250 and 500 mg/Kg body weight of the extract fraction for three days post-inoculation. For toxicological studies, twenty albino rats were randomly divided into four groups (G-J) with five rats each. Rats in group G (control) were orally administered appropriate volume of distilled water while those in groups H-J were orally administered 25, 50 and 100 mg/Kg body weight of the extract fraction respectively for fourteen days. At the end of the experimental period, venous blood was collected and selected tissues isolated and homogenized. The full blood count and activities of alkaline phosphatase, aspartate and alanine aminotransferase in the tissues were determined. The results revealed that the methanolic fraction of C. nucifera (West African Tall variety) husk fibre extract does not possess any antimalarial activity. The extract, at all doses administered, had no significant effect (P>0.05) on the red blood cell indices, white blood cell indices and the activities of all the enzymes in the liver, kidney, heart and brain compared to controls. The results thus suggest that the methanolic fraction of the husk fibre extract may not be responsible for the acclaimed antimalarial action of C. nucifera (West African Tall variety) husk fibre, though it may not aggravate the severity of the disease.Item Evaluation of the Clinico-haematological Characteristics and Pattern of Presentation of Acute Myeloid Leukemia in Ilorin, North Central Nigeria.(Ambrose Alli University, Ekpoma, 2020) Babatunde, A.S.; Durotoye, I.A.; Shittu, A.O.; Omokanye, K.O.; Ogunfemi, M.K.; Owoeye, O.A.; Sanni, E.O.Acute myeloid leukemia (AML) is a malignant haematologicaldisorder of the myeloid lineage in the bone marrow resulting from clonal proliferation of haemopoietic progenitor cells with accumulation of myeloblasts in the blood, bone marrow and tissue infiltration. The aim of this retrospective study was to determine the pattern of presentation, clinical features and haematological parameters of AML patients diagnosed at the Haematology Department of U.I.T.H, Ilorin between 1" January 2008 and 31“ December 2018. The study materials included Bone marrow registers from the department and case folders of all AML patients diagnosed within the study period. Relevant data including socio-demographic characteristics of patients, clinical features at presentation and laboratory tests results were extracted, documented and analyzed. Diagnosis of AML was made based on the findings of 20% blasts in the bone marrow and/or peripheral blood and classified using FAB classification criteria.There were forty-six patients and their median age at diagnosis was 20.6 + 15.9 years.The highest prevalence of the disease was found in the 11 — 20 years age group.The most common presenting symptoms were fever (91.3%), pallor (84.8%), bleeding diasthesis (52.2%), and body weakness (45.7%).The mean+SD values of haematological parameters in the patients wer—e PCV (19.9+5.8%), Hb cone. (6.6+1.9 g/dL), Total WBC (48.1+54.8 x 10’/L) and Platelet Count (39.6+24.0 x 10’/L).The commonest subtype of AML in our study was M4 which was seen in 26 (56.5%) patients, followed by M5 observed in 10 (21.7%) patients. In resource-limited settings such as ours, the diagnosis and monitoring of patients with AML still rely on the clinical and laboratory features of the disease to a large extent, hence the need for Haemato-oncologists and Physicians to be conversant with these features.Item Evaluation of the Indications and Diagnostic Value of Bone Marrow Examination in Haematological Disorders in Ilorin Nigeria: A Review of 496 Cases(College of Health Sciences, University of Ilorin, 2020) Babatunde, A.S.; Durotoye, I.A.; Shittu, A.O.; Omokanye, K.O.; Ogunfemi, M.K; Owoeye, O.A.Abstract: Bone marrow examination, either in the form of aspiration or biopsy, is an invasive procedure which is relatively safe and is still being performed routinely in the investigation and diagnosis of various malignant and non-malignant haematological disorders. The aims of this study were to evaluate the role of bone marrow examination in the investigation and diagnosis of haematological disorders, and the pattern of disorders diagnosed by bone marrow examination in our centre. This study was a retrospective review of all cases of bone marrow examinations which were carried out at the Haematology department of U.I.T.H Ilorin between 1st January 2005 and 31st December 2018. Case folders of all patients who had bone marrow examination done during the study period, Bone marrow aspiration and biopsy registers from Haematology department, Bone marrow reports and Full blood counts (FBC) results were retrieved and analyzed. Relevant clinical information retrieved included age, gender, clinical features at presentation, laboratory investigations, treatment regimen, and survival outcome among others. A total of 496 cases had BME performed during the study period. The patients comprised 276 (55.8%) males and 220 (44.2%) females with a M:F ratio of 1.3:1. Their median age was 38.5 years (age range 16 months and 85 years). Pathological marrow with diagnosis on BME was found in 441 (88.9%) while normal marrow (negative for any pathologic condition) was seen in 55 (11.1%) of patients. The leukemias (acute and chronic) were the commonest haematological disorders found on bone marrow examination, constituting 37.8% of cases. Unexplained anaemia was the commonest clinical feature at presentation necessitating bone marrow examination. Bone marrow examination is still very useful as a diagnostic tool in the hand of Haematologists in the investigation and diagnosis of various haematological disorders, and is relatively safe to perform even with presence of thrombocytopenia.Item Evaluation of the Indications and Diagnostic Value of Bone Marrow Examination in Haematological Disorders in Ilorin Nigeria: A Review of 496 Cases.(College of Health Sciences, University of Ilorin., 2020) Babatunde, A.S.; Durotoye, I.A.; Shittu, A.O.; Omokanye, K.O.; Ogunfemi, M.K.; Owoeye, O.A.Abstract Bone marrow examination, either in the form of aspiration or biopsy, is an invasive procedure which is relatively safe and is still being performed routinely in the investigation and diagnosis of various malignant and non-malignant haematological disorders. The aims of this study were to evaluate the role of bone marrow examination in the investigation and diagnosis of haematological disorders, and the pattern of disorders diagnosed by bone marrow examination in our centre. This study was a retrospective review of all cases of bone marrow examinations which were carried out at the Haematology department of U.I.T.H Ilorin between 1st January 2005 and 31st December 2018. Case folders of all patients who had bone marrow examination done during the study period, Bone marrow aspiration and biopsy registers from Haematology department, Bone marrow reports and Full blood counts (FBC) results were retrieved and analyzed. Relevant clinical information retrieved included age, gender, clinical features at presentation, laboratory investigations, treatment regimen, and survival outcome among others. A total of 496 cases had BME performed during the study period. The patients comprised 276 (55.8%) males and 220 (44.2%) females with a M:F ratio of 1.3:1. Their median age was 38.5 years (age range 16 months and 85 years). Pathological marrow with diagnosis on BME was found in 441 (88.9%) while normal marrow (negative for any pathologic condition) was seen in 55 (11.1%) of patients. The leukemias (acute and chronic) were the commonest haematological disorders found on bone marrow examination, constituting 37.8% of cases. Unexplained anaemia was the commonest clinical feature at presentation necessitating bone marrow examination. Bone marrow examination is still very useful as a diagnostic tool in the hand of Haematologists in the investigation and diagnosis of various haematological disorders, and is relatively safe to perform even with presence of thrombocytopenia.Item Haematological Parameters of Normal Pregnant Women During Antenatal Clinic Booking in Ilorin, North Central Nigeria(Ambrose Alli University, 2017) Babatunde, A.S.; Olawumi, H.O.; Durotoye, I.A.; Shittu, A.O.; Adesina, K.T.; Sani, M.A.; Nyamgee, AAlterations in the values of some haematological parameters like packed cell volume and haemoglobin concentration during pregnancy have been reported to significantly influence pregnancy outcome. The aim of this study was to determine the values of haematological parameters in apparently healthy pregnant women during the antenatal clinic booking visit at the University of Ilorin Teaching Hospital, Ilorin, North central Nigeria. A total of 298 apparently healthy pregnant women who presented for antenatal care booking and satisfied the inclusion criteria were included in the study. There were 64 (21.5%) women in first trimester, 146 (49.0%) women in second trimester and 88 (29.5%) in third trimester; and were aged between 22 and 42 years. Packed cell volume, haemoglobin concentration, Red cell count, mean haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin concentration, Total white blood cell count and differentials, Platelet count and erythrocyte sedimentation rate were carried out in the subjects. The overall mean values and SD of the pregnant women for the haematological parameters were as follows: PCV (32.76±2.18%), Hb (10.82±0.77g/dl), RBC (3.87±2.32 x1012/L), MCH (28.08±2.06 pg), MCV (85.16±5.82 fl), MCHC (33.01±1.05 g/dl), TWBC (7.35±2.62 x109/L), Platelet count (192.15±62.80 x109/L) and ESR (34.07±16.85 mm/hr). There were statistically significant differences in the mean values of PCV, MCH and MCV with increasing gestational age (p<0.05). Conclusion: The present study provides a baseline data on the haematological values in pregnancy in the North central zone of the country on which further large scale studies in future may be based. This study will also be of immense benefit in the monitoring of pregnant women to determine those at risk for timely intervention thereby improving pregnancy outcome.Item Haemostatic Parameters during Pregnancy in Ilorin, Nigeria(College of Health Sciences, University of Ilorin, 2012) Durotoye, I. A.; Babatunde, A.S.; Olawumi, H. O.; Olatunji, P. O.; Adewuyi, J. O.Abstract The objective of this study was to document the effect of pregnancy on some haemostatic parameters (Prothrombin time [PT], activated partial thromboplastin time [APTT], fibrinogen concentration, euglobin clot lysis time [ELT], and platelet count) in different trimesters of pregnancy. One hundred and eighty women with non-complicated pregnancy within the age range of 17-40 years (mean± SD 27.1±3.9years) were recruited for the study and sixty non-pregnant, normotensive apparently healthy age-matched women were used as control. All haemostatic parameters evaluated were determined using standard techniques. Significant differences were observed between the haemostatic parameters studied and controls except for platelet count. Prothrombin time was significantly shorter (p<0.001) and fibrinogen significantly higher (p<0.001) as pregnancy advanced but, other parameters were not influenced by the gestational age. There was a significant difference between the fibrinogen level in older subject compared with subject of younger age group with a mean of 5.5g/l and 4.0g/l (p<0.05) respectively. There was no significant relationship in the haemostatic parameters with parity and educational level of all the subjects. In conclusion, the results of haemostatic screening tests in this study suggest some degree of activation in pregnant women.Item The Impact of Body Mass Index on haematological parameters of pregnant women at booking in Ilorin, Nigeria(University of Ilorin, 2013) Adesina, K.T.; Balogun, O.R.; Aderibigbe, A.S.; Babatunde, A.S.; Sanni, A.; Olarinoye, A.O.; Ezeoke, G.G.ABSTRACT: Background: Pregnancy is associated with physiological changes that affect almost all of the systems in the body, including weight gain; these changes accommodate the demands of the feto-placental unit. Therefore, hematological parameters in pregnancy may not be comparable with those of non-pregnant women. Methodology: A total of 500 pregnant women were consecutively recruited at booking, and 465 met the inclusion criteria. Their blood samples were analyzed for some blood indices, which were compared with their body mass indices and sociodemographic characteristics. Results: The mean levels of Mean Corpuscular Volume(MCV) and Mean Corpuscular Hemoglobin Concentration(MCHC) were 82.2 fl ± 8.4 and 34.0 g/dl ± 1.9, respectively, which were within the normal reference values but close to the lower limits. The hemoglobin concentration was low (10.5 g/dl), whereas the erythrocyte sedimentation rate was high (34.1 mm/hr). Hematological parameters were compared by trimesters. MCV and Mean Corpuscular Hemoglobin (MCH) increased across the trimesters, with P values of 0.0007 and 0.011, respectively (P< 0.05). PCV was also inversely proportional to the gestational age (P0.026). There was no statistically significant difference when BMI and hematological parameters were compared (P>0.05), although RBC and PCV values increased as the maternal weight increased, suggesting a probable positive correlation between the red cell count and concentration and body mass index in pregnancy. Conclusion: This study confirmed the hemodilutional effect of pregnancy and suggests a relationship between BMI, RBC and PCV in pregnancy. Body weight may increase the red cell parameters in pregnancy.