Investigating cerebellar oxidative stress, inflammation and apoptosis following sub-acute MPTP administration in Balb/c mice

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dc.contributor.authorSulaimon, F.A.
dc.contributor.authorAdeniyi, R.A.
dc.contributor.authorAkinnaso, O.A.
dc.contributor.authorOladipupo, O.A.
dc.contributor.authorAwodola, M J.
dc.contributor.authorAdeoye, A. T.
dc.contributor.authorJimoh, T.Y.
dc.contributor.authorShehu,M.
dc.contributor.authorImam, A.L.
dc.contributor.authorIbiyeye, R.Y.
dc.date.accessioned2025-05-02T11:39:04Z
dc.date.available2025-05-02T11:39:04Z
dc.date.issued2025
dc.description.abstractBackground and aim: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administered mice is a known model of Parkinson's disease (PD) which is characterized by neurodegeneration primarily in the substantia nigra; however, increasing evidence highlights the role of the cerebellum in both motor and non-motor symptoms. This study investigates the effects of sub-acute MPTP administration on oxidative stress, inflammation, and apoptosis in the cerebellum of adult male Balb/c mice. Methods: Twenty adult male Balb/c mice, weighing 20-30 g, were acclimatized for 14 days and assigned to control (n=10, PBS) and MPTP groups (n=10, 20 mg/kg body weight of MPTP, intraperitoneally administered daily for five consecutive days). Behavioral assessments were performed using an open field test on day seven, focusing on cerebellar-related behaviors. Following this, histological, immunohistochemical, and biochemical analyses were conducted to evaluate markers of oxidative stress (nuclear factor erythroid 2-related factor 2 along with glutathione peroxidase), inflammation (tumor necrosis factor-alpha as well as nuclear factor kappa B), and apoptosis (B-cell lymphoma 2). Results: MPTP administration significantly reduced nuclear factor erythroid 2-related factor 2 along with glutathione peroxidase while increasing tumor necrosis factor-alpha as well as nuclear factor kappa B in the cerebellum, coinciding with enhanced apoptosis as indicated by a lower B-cell lymphoma 2 level. Behavioral results revealed significant reductions in the grooming frequency of MPTP-treated mice compared to controls. Conclusion: The findings indicate that MPTP induces oxidative stress, inflammation, and apoptosis in the cerebellum of adult male Balb/c mice, it might be worthwhile to observe the cerebellar changes in the diagnosis and management of PD.
dc.identifier.citationSulaimon, F.A., Adeniyi, R.A., Akinnaso, O.A., Oladipupo, O.A., Awodola, M J., Adeoye, A. T., Jimoh, T.Y., Shehu,M., Imam, A.L., & Ibiyeye, R.Y. (2025). Investigating cerebellar oxidative stress, inflammation and apoptosis following sub-acute MPTP administration in Balb/c mice. Journal of Experimental and Clinical Anatomy, 22(1), 44-51.Published by the Society of Experimental and Clinical Anatomists of Nigeria. https://www.ajol.info/index.php/jeca
dc.identifier.urihttps://www.ajol.info/index.php/jeca
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/123456789/15909
dc.language.isoen
dc.publisherSociety of Experimental and Clinical Anatomists of Nigeria.
dc.subjectProinflammatory markers
dc.subjectBalb/c mice
dc.subjectMPTP
dc.subjectCerebellum
dc.subjectParkinson’s disease
dc.titleInvestigating cerebellar oxidative stress, inflammation and apoptosis following sub-acute MPTP administration in Balb/c mice
dc.typeArticle

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