Investigating cerebellar oxidative stress, inflammation and apoptosis following sub-acute MPTP administration in Balb/c mice
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Date
2025
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Journal ISSN
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Publisher
Society of Experimental and Clinical Anatomists of Nigeria.
Abstract
Background and aim: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administered mice is a known
model of Parkinson's disease (PD) which is characterized by neurodegeneration primarily in the substantia nigra;
however, increasing evidence highlights the role of the cerebellum in both motor and non-motor symptoms. This
study investigates the effects of sub-acute MPTP administration on oxidative stress, inflammation, and apoptosis
in the cerebellum of adult male Balb/c mice.
Methods: Twenty adult male Balb/c mice, weighing 20-30 g, were acclimatized for 14 days and assigned to
control (n=10, PBS) and MPTP groups (n=10, 20 mg/kg body weight of MPTP, intraperitoneally administered
daily for five consecutive days). Behavioral assessments were performed using an open field test on day seven,
focusing on cerebellar-related behaviors. Following this, histological, immunohistochemical, and biochemical
analyses were conducted to evaluate markers of oxidative stress (nuclear factor erythroid 2-related factor 2 along
with glutathione peroxidase), inflammation (tumor necrosis factor-alpha as well as nuclear factor kappa B), and
apoptosis (B-cell lymphoma 2).
Results: MPTP administration significantly reduced nuclear factor erythroid 2-related factor 2 along with
glutathione peroxidase while increasing tumor necrosis factor-alpha as well as nuclear factor kappa B in the
cerebellum, coinciding with enhanced apoptosis as indicated by a lower B-cell lymphoma 2 level. Behavioral
results revealed significant reductions in the grooming frequency of MPTP-treated mice compared to controls.
Conclusion: The findings indicate that MPTP induces oxidative stress, inflammation, and apoptosis in the
cerebellum of adult male Balb/c mice, it might be worthwhile to observe the cerebellar changes in the diagnosis
and management of PD.
Description
Keywords
Proinflammatory markers, Balb/c mice, MPTP, Cerebellum, Parkinson’s disease
Citation
Sulaimon, F.A., Adeniyi, R.A., Akinnaso, O.A., Oladipupo, O.A., Awodola, M J., Adeoye, A. T., Jimoh, T.Y., Shehu,M., Imam, A.L., & Ibiyeye, R.Y. (2025). Investigating cerebellar oxidative stress, inflammation and apoptosis following sub-acute MPTP administration in Balb/c mice. Journal of Experimental and Clinical Anatomy, 22(1), 44-51.Published by the Society of Experimental and Clinical Anatomists of Nigeria. https://www.ajol.info/index.php/jeca