Antioxidant, Brine Shrimp Lethality, and Antiproliferative Properties of Gel and Leaf Extracts of Aloe schweinfurthii and Aloe vera
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Date
2017
Authors
Salawu, Kayode
Ayaiyeoba, Edith
Ogbole, Omonike
Adeniji, Johnson
Faleye, Temitope
Agunu, Abdulkarim
Journal Title
Journal ISSN
Volume Title
Publisher
JOURNAL OF HERBS, SPICES & MEDICINAL PLANTS
Abstract
Leaf and gel extracts of Aloe schweinfurthii and A. vera were
subjected to in vitro antioxidant assay using 2,2-diphennyl-1picryl
hydrazyl (DPPH), brine shrimp lethality bioassay, and
cytotoxicity using the MTT assay with two human cancer cell
lines: Rd and Hep-2c. Extracts of A. schweinfurthii gel had IC
values of 44.59; A. vera gel, 41.48, and A. vera leaf, 38.84 µg.mL
,
had similar DPPH radical-scavenging properties and were more
active than A. schweinfurthii leaf. Ascorbic acid had an IC
of
9.26 ± 0.14 µg.mL
–1
. Aloe vera leaf (LC
50
= 325 ± 5.38 µg.mL
)
was more active than the other three extracts in the BSL assay.
The gel extracts of A. schweinfurthii (CC
50
= 4.06 µg.mL
on Rd
and 9.00 µg.mL
–1
on Hep-2c) and A. vera (CC
50
–1
= 4.31 µg.mL
on
Rd, 9.06 µg.mL
–1
on Hep-2c) elicited similar and more potent
cytotoxicity comparable to cyclophosphamide, with CC
= 2.2
µg.mL
–1
on Rd and 2.66 µg.mL
–1
on Hep-2c. Leaf extracts were
less active than gel extracts. This study showed that A. schweinfurthii
gel, A. vera gel and leaf had weak DPPH activity which
were similar. Aloe vera and A. schweinfurthii did not elicit potent
cytotoxicity in BSL assay. The gels from both Aloe species displayed
antipoliferative activity on Rd and Hep-2c, two human
cell cancer lines.
50
50
–1
50
–1
–1
Description
Keywords
BSL; Rd and Hep-2c human cancer cell lines; DPPH radical scavenging