uprizone toxicity and Garcinia kola biflavonoid complex activity on hippocampal morphology and neurobehaviour
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Date
2019-02-26
Journal Title
Journal ISSN
Volume Title
Publisher
Heliyon, Elsevier
Abstract
Cuprizone-induced neurotoxicity has been employed to study the biology of remyelination in experimental
models of multiple sclerosis. This study was aimed at determining the role of kolaviron, a biflavonoid from
Garcinia kola, in mitigating the damaging effects of cuprizone on behaviour and the hippocampus. Twenty-four
male albino mice aged 6–8 weeks were categorised into 4 equal groups: Group A (Control) received regular
diet; Group B received 200 mg/kg/d of kolaviron in addition to their regular diet; Group C received 0.2%
cuprizone diet only, while Group D received both kolaviron and cuprizone diet. The treatment lasted for 35 days
after which behavioural tests (Morris water maze, Y maze and open field tests) were conducted and brain tissues
were processed for histology, histochemistry (Nissl staining), immunohistochemistry (glial fibrillary acidic protein)
and biochemistry (malondialdehyde, superoxide dismutase and glutathione peroxidase). Results showed
that cuprizone toxicity led to weight loss, impairment in memory and exploratory drive, oxidative stress, chromatolysis
and reactive astrocytosis; meanwhile administration of kolaviron prevented cuprizone-induced weight
loss, memory decline, oxidative stress and neuromorphological alterations. In conclusion, administration of
kolaviron might be useful in limiting the effects of cuprizone toxicity on the morphology and functions of the
hippocampus.
Description
Keywords
Anatomy, Biochemistry, Cell biology, Neuroscience, Structural biology
Citation
Omotoso, G.O., Olajide, O.J., Gbadamosi, I.T., Adebayo J.O., Enaibe, B.U., Akinola, O.B. & Owoyele, B.V. (2019): Cuprizone toxicity and Garcinia kola biflavonoid complex activity on hippocampal morphology and neurobehaviour. Heliyon. 5 (2019) e02102. https://www.ncbi.nlm.nih.gov/pubmed/31367687