Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis
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Date
2021-02-12
Authors
TANKO, POLYCARP
SABRI, M.Y.
EMIKPE, B.O.
ONILUDE, O.M.
AJADI, A.A.
Journal Title
Journal ISSN
Volume Title
Publisher
TAYLOR & FRANCIS
Abstract
This study investigated dexamethasone-treatment, shedding
routes, tissue antigen distribution, and pathology of caprine
Brucellosis. Eighteen non-pregnant goats were randomly
grouped into A, B, and C. Group A was administered dexametha-
sone for 7 days at 2 mg/kg before inoculating 0.5 mL
B. melitensis at 107 CFU ocularly while group B was inoculated
0.5 mL B. melitensis only, and C as control negative. Blood
samples, ocular, nasal, and vaginal swabs were obtained for
evaluation. Three goats were sacrificed from each group at
days 21 and 42 post-inoculation (pi) and selected tissues col-
lected for PCR, histopathology, and immunohistochemistry.
Brucella melitensis was detected in the ocular swabs of group
A significantly higher than group B. Shedding was prolonged in
group A compared to B. The overall shedding was 22.2% in
group A and 9.4% in group B. The uterus of both groups
A and B revealed mild inflammation and microgranuloma,
extensive necrotic lesions in lymph nodes. Liver showed multi-
focal necrosis predominantly in group A. Lesion scoring showed
significantly higher scores in A compared to B. Strong immu-
nostaining was observed in the liver, lungs, and spleen, predo-
minantly at day 21 pi. This study demonstrated dexamethasone
prolonged shedding, tissue antigen distribution, and pathology
in dexamethasone-treated goats.
Description
Keywords
Brucellosis, dexamethasone, goats, immunohistochemistry, tissue antigen distribution