Characterisation, Amino Acid Composition and Denaturation status of Acid Soluble Collagen from Catfish (Clarias gariepinus) Skin
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Date
2022-01
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The West Indian Journal of Engineering
Abstract
This study reported characterisation of acid-soluble collagen from the skin of freshwater catfish (Clarias
gariepinus) by Fourier Transform Infra-Red spectroscopy (FTIR), scanning electron microscopy (SEM) and energy
dispersive x-ray (EDX). Amino acid composition and denaturation temperature of the collagen were determined. Acid
soluble collagen (ASC) from the skin of C. gariepinus produced a comparatively yield of 2.38% with all amide
functional groups (Amide A, B, I, II and III) visible in the FTIR spectrum suggesting the intactness of triple helical
structure of the collagen. The SEM of C. glariepinus, shows a mono-fibrillated irregularly arranged crystalline surface
material having 24.46 % carbon, 11.72 % oxygen and 9.40 % nitrogen. The abundance of amino acids follows glycine
> arginine > proline > alanine indicating the integrity of collagen and a non-disruptive method of extraction. The
denaturation temperature (Td) of ASC was about 30 °C implying its usefulness in food and pharmaceutical indus
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Citation
Amide linkage, Clarias gariepinus, Denaturation, Gas chromatograph-pulse flame photometric detector