Determination of Normal and Variant Hemoglobin using Capillary Electrophoresis among Voluntary Blood Donors in North Central Nigeria: Implications on Blood Transfusion Services
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Faculty of Medicine and Health Sciences - Omdurman Islamic University
Abstract
Abstract
Background: Voluntary non-remunerated blood donation is a strategy adopted by
World Health Organization aimed at ensuring safety and adequacy of blood supply.
Sub-Saharan Africa has a high prevalence of hemoglobin disorders and therefore
needs to adopt stringent measures in donor selection to ensure safety for the recipient
of blood transfusion. This study aimed to analyze normal and variant hemoglobin
among voluntary blood donors.
Methods: In this descriptive cross-sectional study, 100 prospective blood donors
including 55 (55%) males and 45 (45%) females, aged 18–34 years were recruited.
Capillary electrophoresis using the Minicap system was used for determining the
hemoglobin variants in alkaline buffer (PH 9.4). Data analysis was done using SPSS
version 20 and p-value < 0.05 was considered as the level of significance
Results: The mean age of the participants was 22.23 ± 3.3 SD years. The proportion
of participants with genotype AA was 67 (67%), those with AS were 17 (22 %), while
those with AC were 11 (11 %). While Hb A ≥ 90% was noted in 67 (67%) blood donors,
Hb S was seen in 22 (22%) and Hb A2 > 3.5% in 57 (57%). Hb F > 2% was observed
in 3% of the studied participants
Conclusion: Variant hemoglobin is common among blood donors and this should
be taken into consideration whenever blood is being crossmatched for recipients of
blood transfusion. Data from this study will be useful in raising awareness and genetic
counseling.
Description
Keywords
prevalence, hemoglobin variants, capillary electrophoresis
Citation
Durotoye, A. I., Salaudeen, A. G., Sanni, E. O., Babatunde, A. S., Durowade, A. K., Olawumi, H. O., Akande, T. M., & Musa, O. I. (2021). Determination of Normal and Variant Hemoglobin using Capillary Electrophoresis among Voluntary Blood Donors in North Central Nigeria: Implications on Blood Transfusion Services. Sudan Journal of Medical Sciences (SJMS), 16(1), 33-42