THE EFFECTS OF SOLVENT EXTRACTS OF CITRULLUS LANATUS SEEDS AND CHRYSOPHYLLUM ALBIDUM COTYLEDONS ON RAT HEPATOCYTE REGENERATION, CYTOCHROME C OXIDASE ACTIVITIES AND BACTERIOCINPRODUCING GRAM-NEGATIVE BACTERIA
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Date
2019-12-30
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PharmacologyOnline
Abstract
Medicinal plants have been shown to exert a plethora of biological and pharmacological which has
been associated with the presence of several bioactive components. In this study, the effects of crude
extracts of Citrullus lanatus seeds (CACL) and Chrysophyllum albidum cotyledons (CACA) on rat
hepatocyte regeneration, liver cytochrome c oxidase activities, and their antimicrobial properties on a
few bacteriocin-producing Gram-negative bacteria were determined. CACL and CACA were screened
for the presence of amino acids using TLC sprayed with ninhydrin, pyridine and modified Commassie
brilliant blue (G250). The amino acids were elucidated by Gas Chromatography-Mass Spectrometry
(GC/MS) and Fourier-transform infrared spectroscopy (FTIR). CACA enhanced tissue regeneration in rat
hepatocytes, in situ and increased the collagen content. CACA also enhanced the activity cytochrome c
oxidase in the liver mitochondrial portion, but inhibited same in the homogenate. CACA and CACL did
not inhibit the bacteriocin-producing bacteria at the different concentrations tested. It is evident that
CACA and CACL contain amino acids that can improve liver antioxidant functions and its mitochondrial
electron transport. Both amino acid-rich fractions, combined with bacteriocin may accelerate aseptic
wound healing.
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Keywords
Tissue regeneration, catalase, cytochrome c oxidase, seed amino acids, Citrullus lanatus seeds, Chrysophyllum albidum cotyledons, bacteriocin-producing bacteria