BIOKEMISTRI
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Date
2003-06-01
Journal Title
Journal ISSN
Volume Title
Publisher
The Nigerian Society for Experimental Biology.
Abstract
Thevetia peruviana seeds cake were defatted and then treated with varying concentrations each
of hydrochloric acid, sodium hydroxide and calcium hydroxide solutions. Each product of
hydrolysis was extracted with chloroform to isolate aglycones, the toxins of the seed. Various
concentrations of hydrochloric acid and sodium hydroxide solution effected complete
detoxification. Only 0.4M and 0.5M of calcium hydroxide solution detoxified the seeds
completely. Albumin and globulin determination by biuret method confirmed that various
concentrations of the hydrolyzing agents increased the quantity of extractable albumin and
globulin in the cake. Each solution used for the detoxification had closely related trend on the
total albumin and globulin value of the treated cake. Higher quantities of albumin and globulin
were recorded in the samples treated with various concentrations of calcium hydroxide solutions.
The study suggests that calcium hydroxide at high concentrations appear to be the best
detoxicant.
Description
Keywords
Thevetia peruviana, detoxification, albumin, globulin