Comparative Antileukemic Activity of a Tetranorditerpene Isolated from Polyalthia longifolia Leaves and the Derivative against Human Leukemia HL-60 cells
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Date
2017
Journal Title
Journal ISSN
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Publisher
Japan Oil Chemists’ Society.
Abstract
Abstract: The discovery of potent cytotoxic isolates from botanicals provides an opportunity to explore this
viable tool for cancer chemoprevention. The antileukemic potential of clerodane diterpene from Polyalthia
longifolia leaves has already been established. However, in this present study, utilizing chromatographic
techniques we report for the first time, the isolation of a rare tetranorditerpene (compound 1) from P.
longifolia. The structure of compound 1 was elucidated and confirmed by spectrophotometric data. UPLCMS
analysis was conducted on the methanolic extract, ethyl acetate fraction, and isolated tetranorditerpene
showed that the tetranorditerpene is one of the major constituents of the plant with a retention time of 30.78
min. In addition, a methyl ester derivative (compound 2) of the isolated tetranorditerpene was synthesized.
Using the CCK-8 assay, we compared the cytotoxic potential of isolated tetranorditerpene (1) and methyl
ester derivative (2) with the previously isolated clerodane diterpenes. Our results showed that the methyl
ester derivative (2) displayed the highest inhibitory activity against human leukemia HL-60 cells. The
isolated tetranorditerpene (1) did not exhibit significant inhibitory effect against HL-60 cells. Morphological
examination indicated chromatin condensation and nuclear fragmentation suggesting induction of apoptosis
in compound 2 treated HL-60 cells. The methyl esterification of the isolated tetranorditerpene (1) conferred
on it a significant level of antileukemic activity suggesting the possibility of a synergistic relationship
between pure compound isolation and synthetic reaction in the discovery of new chemopreventive agents.
Description
Keywords
Polyalthia longifolia,, tetranorditerpene, human leukemic HL-60 cells, antileukemic activity
Citation
18. Afolabi, S., Olorundare, O., Ninomiya, M., Babatunde, A., Mukhtar, H., & Koketsu, M. (2017). Comparative Antileukemic Activity of a Tetranorditerpene Isolated from Polyalthia longifolia Leaves and the Derivative against Human Leukemia HL-60 cells. Journal of Oleo Science, 66 (10):1169–1174.