Comparative Antileukemic Activity of a Tetranorditerpene Isolated from Polyalthia longifolia Leaves and the Derivative against Human Leukemia HL-60 cells

dc.contributor.authorAfolabi, S.
dc.contributor.authorOlorundare, O.
dc.contributor.authorNinomiya, M.
dc.contributor.authorBabatunde, A.
dc.contributor.authorMukhtar, H.
dc.contributor.authorKoketsu, M.
dc.date.accessioned2020-05-22T21:59:22Z
dc.date.available2020-05-22T21:59:22Z
dc.date.issued2017
dc.description.abstractAbstract: The discovery of potent cytotoxic isolates from botanicals provides an opportunity to explore this viable tool for cancer chemoprevention. The antileukemic potential of clerodane diterpene from Polyalthia longifolia leaves has already been established. However, in this present study, utilizing chromatographic techniques we report for the first time, the isolation of a rare tetranorditerpene (compound 1) from P. longifolia. The structure of compound 1 was elucidated and confirmed by spectrophotometric data. UPLCMS analysis was conducted on the methanolic extract, ethyl acetate fraction, and isolated tetranorditerpene showed that the tetranorditerpene is one of the major constituents of the plant with a retention time of 30.78 min. In addition, a methyl ester derivative (compound 2) of the isolated tetranorditerpene was synthesized. Using the CCK-8 assay, we compared the cytotoxic potential of isolated tetranorditerpene (1) and methyl ester derivative (2) with the previously isolated clerodane diterpenes. Our results showed that the methyl ester derivative (2) displayed the highest inhibitory activity against human leukemia HL-60 cells. The isolated tetranorditerpene (1) did not exhibit significant inhibitory effect against HL-60 cells. Morphological examination indicated chromatin condensation and nuclear fragmentation suggesting induction of apoptosis in compound 2 treated HL-60 cells. The methyl esterification of the isolated tetranorditerpene (1) conferred on it a significant level of antileukemic activity suggesting the possibility of a synergistic relationship between pure compound isolation and synthetic reaction in the discovery of new chemopreventive agents.en_US
dc.identifier.citation18. Afolabi, S., Olorundare, O., Ninomiya, M., Babatunde, A., Mukhtar, H., & Koketsu, M. (2017). Comparative Antileukemic Activity of a Tetranorditerpene Isolated from Polyalthia longifolia Leaves and the Derivative against Human Leukemia HL-60 cells. Journal of Oleo Science, 66 (10):1169–1174.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3952
dc.language.isoenen_US
dc.publisherJapan Oil Chemists’ Society.en_US
dc.subjectPolyalthia longifolia,en_US
dc.subjecttetranorditerpeneen_US
dc.subjecthuman leukemic HL-60 cellsen_US
dc.subjectantileukemic activityen_US
dc.titleComparative Antileukemic Activity of a Tetranorditerpene Isolated from Polyalthia longifolia Leaves and the Derivative against Human Leukemia HL-60 cellsen_US
dc.typeArticleen_US

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