Genetic Damage, Biochemical and Histopathological Assessment of Mice Exposed to Cypermethrin and Chlorpyrifos
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Date
2019-03-25
Journal Title
Journal ISSN
Volume Title
Publisher
Centrepoint Journal (Science Edition), Published by University of Ilorin.
Abstract
Chlorpyrifos (CPF) and Cypermethrin (CPM) are insecticides widely used in agriculture because
of their effectiveness against harmful insects but their toxicity to human is of major concern.
This study investigated genotoxic effect of CPF and CPM on some selected organs and tissues of
albino mice. Nine (9) male mice were randomly grouped into three and were administered orally
with CPF and CPM for five consecutive days and thereafter observed for 30 days. Control group
was administered with 0.05ml of distilled water. CPM group was administered with 0.08ml of
CPM while CPF group was administered with 0.05ml of CPF. Our results showed that there was
a significant increase (p>0.05) in both ALT and AST activity in the serum and liver of both CPF
and CPM groups. There was also high concentration of urea, total bilirubin in the serum of both
CPF and CPM groups as well as high concentration of glucose in the brain of CPM group. There
was no significant increase in the concentration of albumin in the liver of CPF and CPM.
Reduction in ALP activity of the liver of both CPF and CPM groups were observed.
Cypermethrin induced an increase in abnormal sperm cell shape and shows significant difference
when compared to chlopyrifos and negative control. The biochemical alterations observed is
consistent with histopathological changes characterized by the appearance of degenerative
necrosis, congestion, inflammation and oedema in the liver and kidney of CPM and CPF groups.
This study shows that these pesticides are potentially genotoxic to animals and are capable of
inducing DNA damage.
Description
Our results showed that cypermethrin and chlorpyrifos may induce genotoxic in mice at certain
concentrations and they should be handled with care to prevent the chemicals being washed into
the aquatic environment where they can enter into the food chain and exert serious health effects
in living organisms including human via systemic genotoxicity.
Keywords
cypermethrin, Chlorpyrifos, Genetic Damage, Histopathological Assessment
Citation
Iyiola, O. A, Sulaiman, F. A., Anifowoshe A. T., Abubakar, F. A., Akolade J.O. (2019). Genetic Damage, Biochemical and Histopathological Assessment of Mice Exposed to Cypermethrin and Chlorpyrifos " Centrepoint journal (Science edition), University of Ilorin, Accepted on the 25th of March. Vol. 23 (2) Page—149-172. Available online at: http://www.unilorin.du.ng/ejournals/index.php/cp or http://www.centrepointjournal.org