MOLECULAR IDENTIFICATION AND ANTIMICROBIAL ACTIVITY OF ENDOPHYTIC FUNGI ASPERGILLUS TAMARII (TRICHOMACEAE)
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Date
2017-03
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Journal ISSN
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Publisher
Nigerian Journal of Pharmaceutical Sciences
Abstract
Microbial drug resistance threatens the effective prevention and treatment of infectious diseases, this has
prompted a continuous search for new and effective antibacterial agents from diverse sources including
endophytic fungi of plants and mushroom. Aspergillus tamarii is an endophytic fungus that has been isolated
from different plants and mushrooms such as Lycoperdon umbrinum. This study was undertaken to identify the
endophytic fungi isolated from L. umbrinum and to evaluate the antimicrobial activity of the extract from the
endophytic fungi. The endophytic fungus was identified as Aspergillus tamarii (strain SRRC 108818S) based on
sequence data analysis of the internal transcribed spacer region of rDNA. The extract from Aspergillus tamarii
had significant antibacterial activities on Salmonella typhi (ATCC33458), Staphylococcus aureus (ATCC6538),
Bacillus subtilis (ATCC6633), Escherichia coli (ATCC25922), with zone of inhibitions of 15.5 ± 0.707, 14.5 ±
0.707, 14 ± 0.00 and 23 ± 1.414 respectively at the highest concentration of 200 mg/mL. The extract however,
does not have any effect on Candida albicans at all tested concentration. Aspergillus tamarii is an endophytic
fungus that could be a potential source of antibacterial agent.
Description
Keywords
Antibacterial activity, Aspergillus tamarii, Endophytic fungi, Lycoperdon umbrinum, Molecular identification