Inhibitory properties of Bambara groundnut protein hydrolysate and peptide fractions against angiotensin converting enzymes, rennin and free radicals
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Date
2016
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Published by Society of Chemical Industry
Abstract
BACKGROUND:Anincreasedrateofhighbloodpressurehasledtocriticalhumanhypertensiveconditionsinmostnations.Inthepresentstudy,bambaraproteinhydrolysates(BPHs)obtainedusingthreedifferentproteases(alcalase,trypsinandpepsin)and theirpeptidefractions(molecularweight:10,5,3and1kDa)wereinvestigatedforantihypertensiveandantioxidantactivities.
RESULTS:Alcalasehydrolysatecontainedthehighestamountoflowmolecularweight(LMW)peptidescomparedtopepsinand trypsinhydrolysates.LMWpeptidesfractions(<1kDa)exhibitedthehighestinhibitoryactivityagainstangiotensin-converting enzyme (ACE) for all the enzymes hydrolysates. For renin inhibition, alcalase hydrolysate showed the highest inhibition at 59%comparedtootherhydrolysatesandtheircorrespondingmembranefractions.Theantioxidantpowerofbambaraprotein hydrolysates and peptide fractions was evaluated through the inhibition of linoleic acid peroxidation and ABTS scavenging activity.Amongthehydrolysates,alcalaseexhibitedthehighestinhibitionoflinoleicacidoxidation.Furthermore,allBPHswere abletoscavengeABTS•+ to a three fold greater extent compared to the isolate.
CONCLUSION: BPH and LMW peptide fractions could potentially serve as useful ingredients in the formulation of functional foods and nutraceuticals against high blood pressure and oxidative stress
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Keywords
Bambara, Protein hydrolysate, Renin, Membrane ultrafiltration, angiotensin-converting enzyme, antioxidant activity