Genome sequencing of endophytic bacterial species associated with Datura stramonium and Sida acuta
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Date
2025
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Publisher
Zagazig University
Abstract
Background: Endophytes are ubiquitous microbes that colonize plants’ tissues without
causing any harm to the host plants, but rather, they confer several adaptable characteristics
on them. They could be of bacterial, fungal, algal, archaeal and actinomycetes origins.
Endophytes also produce useful bioactive metabolites some of which are potent
antimicrobials. In this study, bacterial endophytes were isolated from the leaves of Datura
stramonium and Sida acuta, both of which are important medicinal plants. Methods:
Freshly cut leaves were collected, rinsed and sequentially sterilized. The sterilized leaves
were then cut into smaller pieces and incubated onto extract-seeded nutrient agar medium
augmented with antifungal drug. Pure distinct bacterial colonies were morphologically and
biochemically characterized. Molecular characterization of the strains was done using the
16S rRNA while phylogenetic evolutionary analysis was carried out using the maximum
likelihood method and Tamura-Nei model with MEGA 11 software. Results: A total of 7
bacterial endophytes were isolated and identified, 3 from D. stramonium and 4 from S.
acuta. 16S rDNA genome sequencing of the strains revealed that isolates from D.
stramonium were Pseudomonas aeruginosa, Atlantibacter hermannii and Enterobacter
roggenkampii while those isolated from S. acuta were identified as Pseudomonas
aeruginosa, Pseudomonas monteilii, Enterobacter pseudoroggenkampii and Aeromonas
veronii. Conclusion: The outcome of this research has indicated that numerous strains of
endophytic bacteria inhabit leaves of plants. It is therefore essential to exploit these
endophytic bacteria for their potentials to produce bioactive metabolites and other valuable
antimicrobial products.
Description
Keywords
Endophytes Datura stramonium Sidaacuta genome sequencing antimicrobial products
Citation
Ayoola et al., 2025