Phytochemical, antioxidant and antimicrobial studies of partitioned fractions of Lannea kerstingii Engl. and K. Krause (Anarcadiaceae)
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Date
2021-04-01
Journal Title
Journal ISSN
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Publisher
West African Journal of Pharmacy
Abstract
Background: Antimicrobial resistance is a serious threat to global public health and requires actions like
the development of new antimicrobial with significant activities over existing drugs.
Objectives: This study aims at investigating the phytochemical, antioxidant and antimicrobial
potentials of partitioned fractions of Lannea kerstingii.
Methods: A quantitative phytochemistry of chloroform, ethyl acetate, acetone and methanol fractions
of L. kerstingii for total flavonoid, phenol and alkaloid were done using standard method; antioxidant
and antimicrobial activity were determined using 1,1-Diphenyl-2-picrylhydrazyl and agar diffusion
method respectively.
Results: The chloroform fraction contained only alkaloid (11%) and steroids while all the other fractions
contained phenolic compounds in the range 0.43 to 0.67 mg/g garlic acid. The total flavonoid content
ranged from 0.43 to 0.67 mg/g of quercetin. The total flavonoid in the ethyl acetate was significantly
different from that of the methanol fraction but not with the acetone fraction. The acetone fraction
showed highest antioxidant activity (60.4%) at 0.05 mg/mL though not as comparable to vitamin C. The
ethyl acetate showed high antimicrobial activity as it was active against most of the organisms tested
upon and zone of inhibition ranged from 13±0.02 to 29±0.1 mm. The acetone fraction was active only
against T. mentagrophytes while the methanol fraction showed no activity.
Conclusion: The antioxidant and antimicrobial activities may be due to the presence of flavonoids, as
well as the presence of tannins and terpenoids present in the different fractions. This makes the ethyl
acetate fraction a good source of antioxidant and antimicrobial agent.
Description
Keywords
Quantitative phytochemistry, Antimicrobial assay, Antioxidant assay, Lannea kerstingiI