Exposure to varied cage-size habitats alters pain sensitivity and inflammation-related biomarkers.
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Background: Nature and size of rodent cages vary from one laboratory or country to another. Little is however
known about the physiological implications of exposure to diverse cage sizes in animal-based experiments.
Method: Here, two groups of male Swiss mice (Control group – Cage stationed, and Test group – Cage migrated)
were used for this study. The cage-migrated mice were exposed daily to various cage sizes used across labora tories in Nigeria while the cage-stationed mice exposed daily to different but the same cage size and shape. At the
end of the 30 days exposure, top-rated paradigms were used to profile changes in physiological behaviours, and
this was followed by evaluation of histological and biochemical metrics.
Results: The study showed a significant (p < 0.05) decrease in blood glucose levels (at 60 and 120 min of oral
glucose tolerance test) in the cage-migrated mice compared to cage-stationed mice. Strikingly, peripheral oxi dative stress (plasma malondialdehyde) and pain sensitivity (formalin test, hot-and-cold plate test, and von Frey
test) decreased significantly in cage-migrated mice compared to cage-stationed animals. Also, the pro-in flammation mediators (IL-6 and NF-κB) increased significantly in cage-migrated mice compared to cage-sta tioned mice. However, emotion-linked behaviours, neurotransmitters (serotonin, noradrenaline and GABA),
brain and plasma electrolytes were not significantly difference in cage-migrated animals compared to cage stationed mice.
Conclusion: Taken together, these results suggest that varied size cage-to-cage exposure of experimental mice
could affect targeted behavioural and biomolecular parameters of pain and inflammation, thus diminishing
research reproducibility, precipitating false negative/positive results and leading to poor translational outcomes.
Description
Keywords
Cage Habitat method Pain sensitivity Inflammation
Citation
15. Oyewole, A. L., Oyafemi, K. O., Badmus, K. S., J. O., Omoleye, K. S., Abubakar, M. F., Adeniyi-Raheem, O., Amedu, A., Lawal, D. L., Ijiyode, A. O., Yussuf, A. O., Ishola, S. S., Sulaimon, F. A., Alli-Oluwafuyi, A.O., Nafiu, A.B., Akinola, O., Olajide, O.J., Amin, A., Abdulmajeed, W.I., Michael, O.S., Adeyanju, O.A., & Ogunjimi, G.L. (2020). Exposure to varied cage-size habitats alters pain sensitivity and inflammation-related biomarkers. Journal of Neuroscience Methods, 345,1-10,