Cytoarchitectural differences in reproductive organs of some polycystic ovary-like induced animal models
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Date
2024
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Limited
Abstract
Polycystic ovary syndrome (PCOS) is the most common gynaecological, endocrine disorder that occurs during
reproductive age and is a significant cause of anovulatory infertility. Letrozole is an aromatase inhibitor which
negates the action of the aromatase enzyme, which results in the buildup of male hormones (testosterone) in the
females, causing hyperandrogenism, which is a hallmark of Polycystic Ovarian Syndrome. Mifepristone (RU486)
is a progestin antagonist that acts to arrest the actions of the progesterone hormone, resulting in follicular atresia
and anovulation. DHEA is an androgen which was also administered in a bid to cause hyperandrogenism in the
rats.This study aimed to evaluate the effects of these hormones on the cytoarchitecture of the ovaries and uterus
to assess their various PCOS-like histological features.Animals were grouped mainly into three: Letrozole,
Mifepristone and DHEA groups, which were further divided into two subgroups each, administered low and high
doses of letrozole orally, Mifepristone and Dehydroepiandosterone (DHEA) subcutaneously. Each of the subgroups
also had a comparison control group. Following the completion of administration, the Wistar rats were
euthanized, and their ovaries and uterus were collected for histological analysis.Increased proliferation of
ovarian follicles was noted in the treated groups compared to control, as well as thickening of the endometrial
layer.
Description
Keywords
Hyperandrogenism, Anovulation, Cytoarchitecture, Proliferation, PCOS
Citation
Kadir, E. R., Yakub, A. D., Ojulari, L. S., Hussein, A. O., Lawal, I. A., Jaji-Sulaimon, R., & Ajao, M. S. (2024). Cytoarchitectural differences in reproductive organs of some polycystic ovary-like induced animal models. Tissue and Cell. 89. 102456. Published by Elsevier Limited. Available online at https://www.sciencedirect.com/journal/tissue-and-cell/vol/89/suppl/C