Induction of Micronuclei, Cellular Stress Response, and DNA Strand Breakage in Two Common Fish Species From Rivers and Reservoirs in Ilorin, Nigeria
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Date
2021-08-09
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Abstract
Indiscriminate discharge of home, agricultural, and industrial wastes into water bodies, most rivers and
reservoirs around the world are becoming polluted. The ecotoxicological potential of this in sh species
gathered from important reservoirs and rivers in Ilorin, Nigeria, was explored in this study. Unilorin
reservoir, Asa reservoir, Apodu reservoir, Asa river (Unity), and Asa river (Unity) water samples were
collected and physicochemical characteristics were investigated at ve distinct sites: Unilorin reservoir,
Asa reservoir, Apodu reservoir, Asa river (Unity), and Asa river (Unity) (Harmony). In Tilapia zillii and
Clarias gariepinus, we measured serum biochemical (AST, ALT, ALP, serum ALB), histopathological (gills,
lungs), and serum antioxidant enzyme responses (SOD, CAT, GPx, GR, GST) as a biomarker for oxidative
stress, while micronucleus and comet assays were used to detect DNA damage. Except for DO, which was
very low in the two rivers, the physicochemical parameters and heavy metals evaluated in the ve
separate water bodies were within the allowed levels of the NSDWQ and WHO standard for drinking
water. In comparison to the Unilorin, Apodu, and Asa reservoirs, a slight increase in Pb was observed
across the ve sampling sites, which could contribute to increased biochemical and haematological
pro les, histopathological lesions in the gill and lungs, inductions of MN, NA, and DNA single strand
break in T. zillii and C. gariepinus collected from Asa rivers. This could be due to the indiscriminate
dumping of e uents from adjacent industries, agricultural wastes, and household wastes into rivers.