Effects of seasonal variation on chemical constituents and insecticidal activity of leaf essential oil of Citrus limonium
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Nigerian Society of Biochemistry and Molecular Biology, Nigeria
Abstract
Leaves (500g) of Citrus limonium harvested in the morning (7.00 am) during dry and rainy seasons were separately
hydrodistilled and yielded 0.46 and 0.30 % (w/w) of essential oils. GC and GC-MS analysis revealed that the oils obtained from dry
and rainy season harvests were predominated by monoterpenoids (84.2% and 77.2%). The most abundant constituent of the oils
from dry and rainy season harvests was; limonene (17.9% and 15.3%). Other principal constituents of the oils were; ȕ-pinene (8.0%
and 1.5%), terpinolene (7.0% and 7.1%), citronellal (12.9% and 8.3%), carveol (8.0% and 9.0%), neral (7.9% and 8.5%). citronellol
(4.4% and 5.9%) and ȕ-caryophyllene (3.3% and 5.2%). Contact toxicity bioassay was used to investigate the insecticidal activity of
the oils against Callosobruchus maculatus. Irrespective of the season of harvest the oils were active against the insect. Interestingly,
oils from the two harvests had comparable activity against the adult C. maculatus. Hence, seasonal variation has no significant
effect on insecticidal activity of the oil on the postharvest insect pest.
Description
Keywords
Post-harvest, Citrus limonium, Callosobrochus maculatus, Limonene synthase
Citation
Usman, L.A., Olanipekun, B.E., Ismaeel, R.O., Oguntoye, O.S. & Ojumoola A.O. (2015). Effects of seasonal variation on chemical constituents and insecticidal activity of leaf essential oil of Citrus limonium. Nigerian Journal of Biochemistry and Molecular Biology. 30(2); 126 – 133.