Production of fermentable sugar from locally sourced lignocellulosic biomass by Aspergillus niger isolate
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Department of Science Education, Federal University of Technology, Minna
Abstract
Swithgrass, sunflower stalk, sugarcane baggase, corn cob, corn stover and groundnut shell were pulverized to powder and pre-treated differently with dilute sulphuric acid and sodium hydroxide in preparation for enzymatic conversion to fermentable sugar. Aspergillus niger was isolated from rotten wood, tree bark and soil mixed with saw dust; and screened for cellulase activity using the agar diffusion method with carboxyl methyl cellulose as the substrate. A total of 27 isolates which produced zone of clearance ranging from 4.4 mm to 33.6 mm were obtained. The pre-treated materials were hydrolyzed in a submerged fermentation using the A. niger isolate that produced the highest cellulase activity. Acid pre-treatment was found to be more effective in terms of yield of fermentable sugar. Among the lignocellose materials, swithgrass pre-treated with acid produced the highest sugar yield of 1.80 ± 0.05 mg ml-1 while the lowest yield of 0.38 mg ml-1 was obtained from alkali pre-treated groundnut shell
Description
Keywords
Lignocellulose, pre-treament, Aspergillus niger, fermentable sugar
Citation
11. Saliu, B.K, Sani, A. and Oke, M.A. (2014) Production of fermentable sugar from locally sourced lignocellulosic biomass by Aspergillus niger isolate. Journal of Science Technology and Mathematics Education 10(2): 2-9.