Bioethanol Potentials of Corn Cob Hydrolysed Using Cellulases of Aspergillus niger and Penicillium decumbens
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Date
2012
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Publisher
Leibniz Research Centre for Working Environment and Human Factors, Germany
Abstract
Corn cob is a major component of agricultural and domestic waste in many parts of the world.
It is composed mainly of cellulose which can be converted to energy in form of bioethanol as
an efficient and effective means of waste management. Production of cellulolytic enzymes
were induced in the fungi Aspergillus niger and Penicillium decumbens by growing them in
mineral salt medium containing alkali pre-treated and untreated corn cobs. The cellulases
were characterized and partially purified. Alkali pre-treated corn cobs were hydrolysed with
the partially purified cellulases and the product of hydrolysis was fermented using the yeast
saccharomyces cerevisae to ethanol. Cellulases of A. niger produced higher endoglucanase
and exoglucanase activity (0.1698 IU ml-1 and 0.0461 FPU ml-1) compared to that produced
by P. decumbens (0.1111 IU ml-1 and 0.153 FPU ml-1). Alkali pre-treated corn cob hydrolysed
by cellulases of A. niger yielded 7.63 mg ml-1 sugar which produced 2.67 % (v/v) ethanol on
fermentation. Ethanol yield of the hydrolysates of corn cob by cellulases of P. decumbens was
much lower at 0.56 % (v/v). Alkali pre-treated corn cob, hydrolysed with cellulases of A. niger
is established as suitable feedstock for bioethanol production.
Description
Keywords
Corn cob, endoglucanase, exoglucanase, sugar, ethanol
Citation
Saliu, B.K. and Sani A. (2012): Bioethanol Potentials of Corn Cob Hydrolysed Using Cellulases of Aspergillus niger and Penicillium decumbens EXCLI Journal. 11:468-479. Published by Leibniz Research Centre for Working Environment and Human Factors, Germany. Available online at https://www.excli.de/index.php/excli/past_issues_2012