TAMARIND EXTRACT PRETREATMENT: VALORIZATION OF SUGARCANE BAGASSE FOR CELLULASE PRODUCTION BY Aspergillus flavus
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Date
2021-05-15
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Faculty of science, Obafemi Awolowo University, Ile- Ife
Abstract
Effective pretreatment is crucial for cellulase production from sugarcane bagasse. Pretreatment with tamarind
extract could reduce the hazardous effect associated with chemical pretreatment. The present work investigated
tamarind (Tamarindus indica) extract in combination with H SO and thermal pretreatment of sugarcane bagasse 2 4
for cellulase production by Aspergillus flavus. The sugarcane bagasse was pretreated with tamarind extract pH 2
and pH 4, followed by 1% H SO and thermal treatment at 121°C for 15 min. The pretreatment slurry was 2 4
analysed for reducing sugar while solid bagasse was analysed for weight loss. Aspergillus flavus grew on sugarcane
bagasse under solid state fermentation and the Carboxy Methyl Cellulase (CMCase) and Filter Paper Assay (FPA)
activities were compared on the various pretreatments. The pretreatments changed the visible morphology of
the sugarcane bagasse observed by the swelling, fibrous appearance and colour change. Pretreatment slurry
yielded highest soluble reducing sugar at 60.01 mg/ml in tamarind extract (pH 4/1% H SO ) and highest weight 2 4
loss of solids at 73.70% in tamarind extract (pH 2/1% H SO /thermal 121°C). Aspergillus flavus performed better 2 4
on tamarind extract (pH 2/1% H SO ) by producing optimal CMCase and FPA activities at 0.100 U/ml and 2 4
0.409 U/ml respectively after 3 days of fermentation. Cellulase was maximally active at temperature of 50 °C.
The tamarind extract pretreatment successfully proved to be an alternative organo-chemical pretreatment of
sugarcane bagasse as evidenced by the physical properties, soluble reducing sugars and cellulase activities
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Citation
Kazeem, M. O., Ajijolakewu, K. A., Ahmed El - Imam, A M. & Salau, R. B. (2021): Tamarind extract pretreatment: valorization of sugarcane Bagasse for cellulase production by Aspergillus flavus. Ife Journal of Science 23(2); 1-10