Browsing by Author "Yakubu, M.T."

Now showing 1 - 5 of 5
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    Amelioration of haematological alterations in streptozotocin-induced diabetic rats by aqueous leaf extract of Senna fistula.
    (Faculty of Basic Medical Sciences, Lagos State University College of Medicine, Ikeja, Nigeria, 2018) Ayinla M.T; Owoyele, B.V.; Yakubu, M.T.; Bilamin, S.
    Objectives: This study investigated the effects of aqueous leaf extract of Senna fistula on some haematological parameters, glucose-6-phosphate dehydrogenase activity, lipid peroxidation and osmotic fragility changes in streptozotocin-induced diabetic rats. Methods: Thirty albino rats were divided into six groups (A-F). such that A was the control non-diabetic, B was the diabetic that received distilled water, C was the diabetic group treated with 2.5 mg/kg body weight of glibenclamide while animals in groups D-F were made diabetic and treated with different doses (28.57, 57.14 and 114.28 mg/kg body weight) of the extract for 28 days. Results: Packed cell volume (PCV), red blood cell count (RBC), haemoglobin (Hb) and platelets count were decreased in diabetic untreated rats, but administration of the extract significantly increased (p<0.05) these values. Treatment with aqueous leaf extract of Senna fistula also increased the resistance of erythrocytes to lysis as evidenced by the median mean corpuscular fragility (MCF50) of 0.72% in diabetic rats and 0.58%, 0.57% and 0.58% after administration of the extract. Similarly, treatment with aqueous leaf extract of Senna fistula decreased the elevated Malondialdehyde level (p<0.05) of diabetic rats. Glucose-6-phosphate dehydrogenase activity increased significantly in the extract-treated groups and these compared favourably with the control non-diabetic and glibenclamide-treated groups. Conclusion: This study concludes that the extract might improve the diabetic-induced disturbances of some haematological parameters by reducing lipid peroxide level and susceptibility of red blood cells to haemolysis.
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    Anti-diabetic Activity of Aqueous Extract of Senna alata (Fabacea) Flower in Alloxan-induced Diabetic Male Rats
    (Published by Cameroon Biosciences Society, 2016) Yakubu, M.T.; Uwazie, N.J.; Igunnu, Adedoyin
    This study investigated the acclaimed anti-diabetic potential of aqueous extract of Senna alata flower in alloxan-induced diabetic rats. Forty-two, male Wistar rats were randomized into six groups (I-VI) such that animals in group I which were non-diabetic received 0.5ml each of distilled water while those in groups II-IV which were induced into diabetes (intraperitoneal administration of 150 mg/kg body weight of alloxan) received orally 0.5ml each of distilled water, glibenclamide (2.5 mg/kg body weight), 25, 50 and 75mg/kg body weight of the extract respectively once daily for 13 days. The secondary metabolites in decreasing order of abundance were phenolics, flavonoids, cardiac glycosides, steroids, saponins, anthraquinones, tannins and alkaloids. The elevated (P < 0.05) blood glucose level, total cholesterol, triglycerides, low density lipoprotein-cholesterol, haemoglobin, total bilirubin, serum urea and creatinine as well as the reduced body weight, high density lipoprotein-cholesterol, liver glycogen, packed cell volume, albumin, conjugated bilirubin and hexokinase activity by the alloxan were reversed and or/ameliorated by the extract, similar to the reference drug, glibenclamide. The extract restored the alloxan treatment related distortion and atrophy in the pancreas. The available data in the study suggests that aqueous extract of Sena alata flower at the doses of 25, 50 and 75mg/kg body exhibited anti-diabetic activity with the highest dose displaying the most profound activity. The anti-diabetic activity conferred by the secondary metabolite is suggested, among other, to be via enhancing the activity of hexokinase. This study has justified the folkloric use of S. alata flower in the management of diabetes.
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    Anti-diabetic activity of aqueous extract of Senna fistula leaves in streptozotocin-induced diabetic rats
    (Nigerian Society of Biochemistry and Molecular Biology, 2014) Ayinla M.T; Owoyele, B.V.; Yakubu, M.T.
    Abstract: This study aims at evaluating the antidiabetic effect of chronic administration of agueous extract of Senna fistula leaves in streptozotocin-induced diabetic rats. Thirty albino rats (110-140 g) were randomized into 6 groups (A-F) Animals in Group A was the non-diabetic control group, B was the diabetic that received distilled water, C was the diabetic animals treated with 2.5 mg/kg body weight of glibenclamide while animals in groups D, E and F were made diabetic and treated with different doses (28.57,57.14 and 114.28 mg/kg body weight) of the extract for 28 days. The results showed that the blood glucose level of diabetic rats were significantly and progressively reduced in the extract and glibenclamide treated animals when compared with the diabetic animals that received distilled water. The urine glucose and ketones which were detected in diabetic animals treated with distilled water disappeared in the extract treated animals comparable to the diabetic animals that received the reference drug.Also there was significant reduction in the postprandial blood glucose of diabetic treated animals when compared with diabetic animals that were administered distilled water throughout the exposure period. Furthermore, the level of albumin was normalized by the extract in a manner similar to those treated with glibenclamide. The significant increases in plasma cholesterol, low density lipoprotein and urea in the diabetic animals that received distilled water were reverted back to their respective control values by the extract. The high density lipoprotein was significantly different in all the treated animals. It can therefore be concluded that the oral administration of aqueous extract of Senna fistula leaves possess antihyperglycemic activity and it is also effective in controlling some metabolic disturbances associated with diabetes.
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    Antihyperglyceamic effects of aqueous leaf extract of Senna fistula in streptozotocine-induced diabetic rats
    (School of Medicine and Health Sciences, University of Papua New Guinea, 2019) Ayinla M.T; Owoyele, B.V.; Yakubu, M.T.; Biliamin, S.A.
    This study investigated antihyperglycemic effects of chronic administration of aqueous leaf extract of Senna fistula in Streptozotocin-induced diabetic rat. Thirty rats were randomly assigned into six groups (A-F). Animals in group A were the control non-diabetic, in group B were diabetic and received distilled water, in group C were diabetic, treated with 2.5 mg/kg body weight of Glibenclamide, while animals in groups D, E and F were diabetic treated with 28.57, 57.14 and 114.28 mg/kg body weight respectively of aqueous leaf extract of Senna fistula for 28 days. At the end of 28 days blood samples were collected for the assay of Insulin, Superoxide Dismutase, Catalase, and Glutathione Peroxidase in serum and liver Glycogen. The result showed that the blood glucose levels of diabetic rats were significantly reduced in the extract and Glibenclamide treated animals when compared with diabetic rats that received distilled water. Similarly, there was a significant increase in serum Insulin level, Superoxide dismutase, Catalase and Glutathione peroxidase activities and liver glycogen in the extract and Glibenclamide treated diabetic groups when compared with diabetic untreated group. The results indicated that oral administration of aqueous extract of Senna fistula has antihyperglycemic effect by stimulating Insulin secretion and activating antioxidant enzymes.
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    Comparative Study on the Antioxidant Activities of Ethyl acetate and Methanolic Leaf Extracts of Celosia argentea
    (Nigeria Society of Biochemistry and Molecular Biology, 2014) Malomo, S.O.; Yakubu, M.T.; Amira, O.J.; Sulyman, A.O.; Dosumu, O.O.; Igunnu, Adedoyin; Oluwaniyi, O.O.; Arise, R.O.; Adebayo, J.O.
    The present study was carried out to compare the secondary metabolites, in vitro and in vivo antioxidant activities as well as the safety of ethyl acetate and methanolic extracts of Celosia argentea leaves in cadmium-induced oxidative stress in rats. The secondary metabolite screening was done by standard methods while the in vitro antioxidant activity of the extract was evaluated using ammonium thiocyanate, reducing power and diphenyl picryl hydrazyl (DPPH) radical scavenging models. In the in-vivo antioxidant and toxicological studies, thirty rats (Rattus novergicus) weighing 137.05 ± 5.84g were completely randomized into six groups (A-F) of five animals each. Animals in group A received orally 0.5ml of distilled water for 7 days while those in groups B, C, D, E and F received same volume corresponding to 8 mg/kg body weight (bw) of cadmium, in addition to simultaneous administration of distilled water, 100 mg/kg b.w of ascorbic acid, 100, 200 and 400 mg/kg b.w. of the extract respectively. Biochemical indices of in vivo antioxidant activities and toxicity were evaluated in the animals after the treatment period. The ethyl acetate extract of C. argentea contained saponins (1.67%), tannins (0.65%), cardenolide and dienolides (1.20%) and phenolics (0.42%) whereas the methanolic extract contained saponins (3.20%), tannins (0.65%), cardenolide and dienolides (0.006%) and phenolics (5.72%). Reducing sugar, steroids, and glycosides were only detected in the ethylacetate extract. The ethyl acetate extract and ascorbic acid, at 50 mg/ml, inhibited linoleic acid oxidation by 51.00 and 24.2% respectively whereas the methanolic extract produced 51.01% inhibition. Ethylacetate extract at 10, 50 and 100 mg/ml produced reducing power of 0.116, 0.092 and 0.127 nm whereas the methanolic extract produced 0.131, 0.185 and 0.183nm when compared with ascorbic acid that gave 0.092, 0.089 and 0.107 nm. The 100 μg/ml of both the ethyl acetate and methanolic extracts scavenged 82% and 30% respectively of the DPPH radical as against 65% in ascorbic acid. Both the extracts attenuated the cadmium chloride treatment related reduction in the activities of superoxide dismutase, catalase, alkaline phosphatase, gamma glutamyl transferase, alanine and aspartate transaminase as well as the levels of uric acid, albumin, total and conjugated bilirubin, total protein and the Cd elevated levels of malondialdehyde in the serum and tissues of the animals in a manner similar to that of the ascorbic acid treated animals and the non-Cd treated animals administered distilled water; with the ethyl acetate producing a better result. The totality of the results conferred antioxidant activity on the ethyl acetate extract and methanolic extract by the phenolic components of the extracts via induction of the antioxidant enzymes and scavenging of free radical. The extracts also reversed cadmium induced changes in the biomarkers of liver damage.