Browsing by Author "Yakubu, M.T"

Now showing 1 - 4 of 4
  • Item
    Brassica oleracea (cabbage)-based diet ameliorates high fat diet-induced hyperlipidaemia in rats
    (Library and Publication Committee, University of Ilorin, Ilorin, Nigeria, 2014-01) Oloyede, H.O.B; Aina, G.O; Salawu, M.O; Ajiboye, T.O; Oladiji, A.T; Yakubu, M.T; Nafiu, M.O
  • Item
    Effect of administration of Ibuprofen on the levels of parasitaemia, albumin and total protein concentration in rats infected with Trypanosoma bruce brucei
    (African Scientists, 2012) Sulaiman, F.A; Akanji, M.A; Yakubu, M.T
    Trypanosoma brucei brucei-infected rats were administered intraperitoneally with ibuprofen at the dose of 0.02mg/kg body weight, thrice daily before infection (prophylaxis), three and ten days post infections (early and late stage respectively). Two sets of control groups were set up in parallel with the test groups viz: positive and negative controls. Parasiataemia was monitored on daily basis. The animals were sacrificed using diethyl ether, albumin (Grant et al., 1987) and the protein concentrations (Tietz, 1995) were determined in the liver, kidney, brain and serum using standard methods. Albumin and total protein concentrations were significantly (P< 0.05) reduced in the tissues of the rats in the late stage compared to the positive control and treated groups while the concentrations of the biomolecules were significantly elevated (P>0.05) in the serum of the late stage group compared to those of the positive control and treated groups. It was concluded that Ibuprofen could be a good candidate for the management of African sleeping sickness. Ibuprofen significantly (P<0.05) reduces blood parasitaemia compare to the controls and extended the life span of the rats by 2, 4 and 7 days in the late, early and prophylactic stages respectively.
  • Item
    Effects of aqueous extract of Cissus populnea stem on function indices of the ovary and uterus of female Wistar rats
    (Library and Publication Committee, University of Ilorin, Ilorin, Nigeria, 2014) Yakubu, M.T; Saliman, S.S; Abdul Rasheed, R.O; Opakunle, M.F; Jimoh, R.O; Nafiu, M.O
  • Item
    Laboratory manual for practical Biochemistry
    (Department of Biochemistry, Faculty of Life Science, University of Ilorin, 2015-07) Akanji, M.A; Oloyede, H.O.B; Bewaji, C.O; Balogun, E.A; Malomo, S, O; Oladiji, A.T; Yakubu, M.T; Adebayo, J.O; Arise, R.O; Sulaiman, F.A; Igunnu, A; Nafiu, M.O; Salawu, M.O; Quadri, A.L; Oyegoke, R.A; Muritala, H.F; Abubakar, F.A; Modupe Olusegun; Soji-Omoniwa; Bello, O.K; Omar, Sikemi
    PREFACE I am glad to present this edition of Laboratory Manual For Practical Biochemistry on behalf of the academic staff members of the Department of Biochemistry, Faculty of Life Sciences,University of llorin,Ilorin, Nigeria. In August 2014, when I assumed the headship of the Department of Biochemistry,the need for increased emphasis on practical aspects of Biochemistry in all areas necessitated the design of Laboratory Manual for undergraduate curriculum. The aim is to produce a 'text guide'that provides students basic appreciation of the underlying principles and practical strategies of the analytical and preparative techniques that are fundamental to the study and understanding of Biochemistry.Adequate attention has been given to those techniques that students often encounter in their practical classes. The content of this text were written in such a way that even the average students can read and understand. Regarding the organization, the book consists of seven(7)sections. Section One deals with Basic Laboratory Ethics,presenting an overview of the departmental policy. laboratory ethies and instructions about results recording and presentation. Section Two is divided into Practicals 1, 2 and 3 covering handling of experimental animals and preparation of solutions with exercises to assess students'understanding. Section Three presents Practical 4 which dwells on amino acids and proteins, highlighting their specific reactions and tests:Practical 5 is on absorption spectra of pure substances.and estimation of urinary creatinine. Practical 6 describes tests for precipitation of proteins by heating,organic acids, mineral acids, ethunol and salts of heavy metals while Practical 7 dwells on isolation, puritication and identification of amino acids and proteins employing puper chromatography,gel filtration chromatogruphy and iscelectric point determination. Section Four is on enzymes and it is divided into Pructicals 8.9.10 and 11 with experiments ranging from pH effects-on enzyme activity to specific reactivity for the recognition of some enzymes.Section Five hus two Practicals (12 and 13) on vitamins. Section Six is divided into Practicals14.15.16.17and 18 covering carbohydrate and nucleotide metabolisi presenting tests for glucose, Inctic acid, pyruvic acid and estimation of RNA while Section Seven is on lipid metabolism and is subsectioned into Pructicals 19.20,21,22. 23 and 24.covering solubility and qualitative tests on lipids. TLC.reactions of bile acids and pigments,determinution of ketone bodies and cholesteroIin serum. On behalf of academic staff members of the Department of Biochemistry,I wish to appreciate the support and approval for the publication and production of this text by the Vice-Chancellor,Prof.Abdulganiyu Ambali,OON.The efforts of all academic and technical Staff of the Department of Biochemistry is also acknowledged. Dr. R. O. Arise Ag.Head.Department of Biochemistry. University of llorin.llorin. July.2015 08052261156