Browsing by Author "Yahya, Rahmat"
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Item Effects of MOF6 Fraction from Ethanolic Extract of the Leaves of Moringa oleifera against Sodium Arsenite-Induced Hepatotoxicity in Rats(Ethiopian Pharmaceutical Association, 2020) Ameen, Mubarak; Adelaja, Akinlolu; Mukadam, Abdulhamid; Muheen, Biliaminu; Ajiboye, Olaolu; Yahya, Rahmat; Abdulrahman, Bashir; Oyetunji, Oyepeju; Rotimi, Mojishola; Nejo, Victoria; Omotoso, GabrielMoringa oleifera (MO) is a plant of significant medicinal importance. The dried leaves of MO were pulverized, extracted with ethanol and fractionated using column chromatography to provide seven fractions (MOF1-7) with MOF6 having the best preliminary antioxidant potential. Therefore, this study evaluated the hepatoprotective potentials of MOF6 in sodium arsenite (SA)-induced hepatotoxicity in rats. Thirty-five adult male Wistar rats were randomly divided into seven groups of five rats each. Control Group I received normal saline. Groups II and III received 20 mg/kg body weight (bw) of SA for 3 and 6 weeks, respectively. Groups IV and V received 20 mg/kg bw of SA for 3 weeks followed by treatment with 5.0 and 7.5 mg/kg bw of fraction MOF6, respectively, for 6 weeks. Groups VI and VII received only 5.0 and 7.5 mg/kg bw of fraction MOF6, respectively, for 6 weeks. Antioxidant (lipid peroxidation) and biochemical analyses of liver enzymes of all rats were carried out after the completion of experimental procedures. Results showed statistically significant lower mean values (p ≤ 0.05) of malondialdehyde (MDA), acid phosphatase (ACP) and γ-glutamyl transferase (GGT) in rats of Groups IV and V compared with Group III. However, there were statistically significant higher mean values (p ≤ 0.05) of alkaline phosphatase (ALP) in Groups IV and V compared with Groups I and III. In conclusion, these results implied that fraction MOF6 has antioxidant and hepatoprotective potentials. However, results of ALP analyses implied that MOF6 possibly augmented SA-induced hepatotoxicity in rats.Item Extraction, isolation and evaluation of anti-toxic principles from Moringa oleifera (MOF6) and Myristica fragrans (Trimyristin) upregulated Acetylcholinesterase concentrations in Sodium arsenite-induced neurotoxicity in rats.(National Institute for Pharmaceutical Research and Development, 2020-12) Akinlolu, Adelaja; Ameen, Mubarak; Quadri, Tobilola; Odubela, Kayode; Omotoso, Gabriel; Yahya, Rahmat; Biliaminu, Sikiru; Adeyanju, Muinat; Ebito, Gabriel; Otulana, JubrilThis study evaluated the neuroprotective effects of MOF6 (isolated from Moringa oleifera leaves) and Trimyristin (isolated from Myristica fragrans seeds) on Acetylcholinesterase concentrations in cerebral cortices of rats with Sodium arsenite-induced neurotoxicity. Sixty-five adult male rats (150 g-250 g) were randomly divided into thirteen groups comprising of five rats per group. Groups 1 and 3 received physiological saline and 1 ml/200 g bodyweight of Olive oil respectively for 9 weeks. Group 2 received 20 mg/kg bodyweight of Sodium arsenite (SA) for 6 weeks and left untreated for another 3 weeks. Groups 4-5 received 20 mg/kg bodyweight of SA for 3 weeks followed by treatments with 5.0 and 7.5 mg/kg bodyweight of MOF6 respectively for 6 weeks. Groups 6-7 received 20 mg/kg bodyweight of SA for 3 weeks followed by treatments with 15 and 30 mg/kg bodyweight of Trimyristin respectively for 6 weeks. Groups 8-11 received 5.0 and 7.5 mg/kg bodyweight of MOF6; 15 and 30 mg/kg bodyweight of Trimyristin respectively for 9 weeks. Groups 12-13 received 7.5 mg/kg bodyweight of MOF6 and 30 mg/kg bodyweight of Trimyristin respectively for 6 weeks followed by co-administration of each extract dose with 20 mg/kg bodyweight of SA for another 3 weeks. Histological examination of cerebral cortices and biochemical analyses of Acetylcholinesterase concentrations were carried out in all rats. Computed data were analyzed using Microsoft Excel 2016 with statistical significance at p≤0.05. Histopathological evaluations revealed normal histo-architecture of cerebral cortices of all rats. Results showed statistically significant (p≤0.05) increases in Acetylcholinesterase concentrations in rats of Groups 1-10 and 12 compared with Group 2 (2.78±1.76 𝜇mole/min/g). 7.5 mg/kg bodyweight of MOF6 showed the best therapeutic and neuro-regenerative potential against SA-induced neurotoxicity. Conclusions: Our findings implied that MOF6 and Trimyristin reversed downregulation of Acetylcholinesterase concentrations in SA-induced neurotoxicity in rats; and possess neuro-protective and neuro-regenerative potentials.