Extraction, isolation and evaluation of anti-toxic principles from Moringa oleifera (MOF6) and Myristica fragrans (Trimyristin) upregulated Acetylcholinesterase concentrations in Sodium arsenite-induced neurotoxicity in rats.
No Thumbnail Available
Date
2020-12
Journal Title
Journal ISSN
Volume Title
Publisher
National Institute for Pharmaceutical Research and Development
Abstract
This study evaluated the neuroprotective effects of MOF6 (isolated from Moringa oleifera leaves) and
Trimyristin (isolated from Myristica fragrans seeds) on Acetylcholinesterase concentrations in cerebral
cortices of rats with Sodium arsenite-induced neurotoxicity. Sixty-five adult male rats (150 g-250 g)
were randomly divided into thirteen groups comprising of five rats per group. Groups 1 and 3 received
physiological saline and 1 ml/200 g bodyweight of Olive oil respectively for 9 weeks. Group 2 received
20 mg/kg bodyweight of Sodium arsenite (SA) for 6 weeks and left untreated for another 3 weeks.
Groups 4-5 received 20 mg/kg bodyweight of SA for 3 weeks followed by treatments with 5.0 and 7.5
mg/kg bodyweight of MOF6 respectively for 6 weeks. Groups 6-7 received 20 mg/kg bodyweight of SA
for 3 weeks followed by treatments with 15 and 30 mg/kg bodyweight of Trimyristin respectively for 6
weeks. Groups 8-11 received 5.0 and 7.5 mg/kg bodyweight of MOF6; 15 and 30 mg/kg bodyweight of
Trimyristin respectively for 9 weeks. Groups 12-13 received 7.5 mg/kg bodyweight of MOF6 and 30
mg/kg bodyweight of Trimyristin respectively for 6 weeks followed by co-administration of each
extract dose with 20 mg/kg bodyweight of SA for another 3 weeks. Histological examination of cerebral
cortices and biochemical analyses of Acetylcholinesterase concentrations were carried out in all rats.
Computed data were analyzed using Microsoft Excel 2016 with statistical significance at p≤0.05. Histopathological
evaluations revealed normal histo-architecture of cerebral cortices of all rats. Results
showed statistically significant (p≤0.05) increases in Acetylcholinesterase concentrations in rats of
Groups 1-10 and 12 compared with Group 2 (2.78±1.76 𝜇mole/min/g). 7.5 mg/kg bodyweight of MOF6
showed the best therapeutic and neuro-regenerative potential against SA-induced neurotoxicity.
Conclusions: Our findings implied that MOF6 and Trimyristin reversed downregulation of
Acetylcholinesterase concentrations in SA-induced neurotoxicity in rats; and possess neuro-protective
and neuro-regenerative potentials.
Description
Keywords
Sodium arsenite, Neurotoxicity, Acetylcholinesterase, Moringa oleifera, Myristica